Restriction map for
pFN21AB8295
(Circular) (Six-base) MAPSORT of: pFN21AB8295.seq Check: 5955 from: 1 to: 6124
pFN21AB8295 6124 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2053
Size: 6124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 2160 3445 2160
Size: 1285 4839
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2364 2670 3178 4187 5571 5839 1632
Size: 499 233 306 508 1009 1384 268 1917
Fragments arranged by size:
1917 1384 1009 508 499 306 268 233
AfeI AGC'GCT
Cuts at: 2144 2341 2144
Size: 197 5927
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2919 5023 6000 1203
Size: 1716 2104 977 1327
Fragments arranged by size:
2104 1716 1327 977
AgeI A'CCGG_T
Cuts at: 2431 3421 4955 2431
Size: 990 1534 3600
Fragments arranged by size:
3600 1534 990
AhdI GACnn_n'nnGTC
Cuts at: 2402 4853 2402
Size: 2451 3673
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 6092
AlwNI CAG_nnn'CTG
Cuts at: 2257 2284 2462 3867 5439 2257
Size: 27 178 1405 1572 2942
Fragments arranged by size:
2942 1572 1405 178 27
ApaI G_GGCC'C
Cuts at: 2045 2535 2045
Size: 490 5634
ApaLI G'TGCA_C
Cuts at: 2923 4181 5337 2923
Size: 1258 1156 3710
Fragments arranged by size:
3710 1258 1156
ApoI r'AATT_y
Cuts at: 1493 3433 3659 3811 5920 1493
Size: 1940 226 152 2109 1697
Fragments arranged by size:
2109 1940 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 2231 2476 3449 1870
Size: 73 238 50 245 973 4545
Fragments arranged by size:
4545 973 245 238 73 50
AvrII C'CTAG_G
Cuts at: 2045 2045
Size: 6124
BamHI G'GATC_C
Cuts at: 3144 3454 3821 3144
Size: 310 367 5447
Fragments arranged by size:
5447 367 310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2160 3076 3445 4900 618
Size: 325 217 627 373 916 369 1455 1842
Fragments arranged by size:
1842 1455 916 627 373 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2307 2535 2664 3038 3443 1391
Size: 654 262 228 129 374 405 4072
Fragments arranged by size:
4072 654 405 374 262 228 129
BbeI G_GCGC'C
Cuts at: 3080 3080
Size: 6124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5153
BbvCI CC'TCA_GC
Cuts at: 2225 3212 2225
Size: 987 5137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2467 2501 4315 4349 1903
Size: 34 530 34 1814 34 3678
Fragments arranged by size:
3678 1814 530 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2368 6119 2368
Size: 3751 2373
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2535 2927 4185 5341 1790
Size: 255 490 392 1258 1156 2573
Fragments arranged by size:
2573 1258 1156 490 392 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 2913 3162
Size: 416 53 83 399 24 610 886 249
Cuts at: 3162 3855 4813 442
Size: 693 958 1753
Fragments arranged by size:
1753 958 886 693 610 416 399 249
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2279 2291 2294 2393 3426 4784 1239
Size: 908 132 12 3 99 1033 1358 2579
Fragments arranged by size:
2579 1358 1033 908 132 99 12 3
Bpu10I CC'TnA_GC
Cuts at: 2192 2225 3212 2192
Size: 33 987 5104
Fragments arranged by size:
5104 987 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2559 4249 5114 5412 2559
Size: 1690 865 298 3271
Fragments arranged by size:
3271 1690 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2410 2498 4787 882
Size: 617 911 88 2289 2219
Fragments arranged by size:
2289 2219 911 617 88
BsaAI yAC'GTr
Cuts at: 493 1204 2920 3915 493
Size: 711 1716 995 2702
Fragments arranged by size:
2702 1716 995 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3077 3931 4313 4948
Size: 53 83 186 936 1544 854 382 635
Cuts at: 4948 275
Size: 1451
Fragments arranged by size:
1544 1451 936 854 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2431 3236 3421 4557 4955 5229 5376 5787 2431
Size: 805 185 1136 398 274 147 411 2768
Fragments arranged by size:
2768 1136 805 411 398 274 185 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 3272 3302 1178
Size: 30 165 30 1149 30 690 30 4000
Fragments arranged by size:
4000 1149 690 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2351 2403 2443 2573 1186
Size: 919 246 52 40 130 4737
Fragments arranged by size:
4737 919 246 130 52 40
BseYI C'CCAG_C
Cuts at: 2613 5327 5823 2613
Size: 2714 496 2914
Fragments arranged by size:
2914 2714 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4948
Fragments arranged by size:
4948 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2307 2927 3038 4185 4270 5341 2211
Size: 96 620 111 1147 85 1071 2994
Fragments arranged by size:
2994 1147 1071 620 111 96 85
BsmI GAATG_Cn'
Cuts at: 3066 3635 3728 3066
Size: 569 93 5462
Fragments arranged by size:
5462 569 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2821 2821
Size: 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2211 2307 2535 2664 2927 3038
Size: 399 255 166 96 228 129 263 111
Cuts at: 3038 3443 4185 4270 5341 1391
Size: 405 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 263 255 228
166 129 111 96 85
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3479 844
Size: 1209 1426 3489
Fragments arranged by size:
3489 1426 1209
BsrBI CCG'CTC
Cuts at: 1394 1823 2377 4011 1394
Size: 429 554 1634 3507
Fragments arranged by size:
3507 1634 554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2326 4619 4793 65
Size: 1183 1078 2293 174 1396
Fragments arranged by size:
2293 1396 1183 1078 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2431 2994 3421 4768 4955 1700
Size: 110 621 563 427 1347 187 2869
Fragments arranged by size:
2869 1347 621 563 427 187 110
BsrGI T'GTAC_A
Cuts at: 96 2538 96
Size: 2442 3682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 2618 4184 5196 2618
Size: 1566 1012 3546
Fragments arranged by size:
3546 1566 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstXI CCAn_nnnn'nTGG
Cuts at: 2552 2552
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 2235 2253 2368 2578 3144 3454 3821 4206
Size: 263 18 115 210 566 310 367 385
Cuts at: 4206 4223 5675 5761 5773 6119 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 566 385 367 346 310 263
210 115 86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2455 2703 2455
Size: 248 5876
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5572
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2729 3640 4434 4454 2729
Size: 911 794 20 4399
Fragments arranged by size:
4399 911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2481 2516 3079 3114 529
Size: 35 1917 35 563 35 3539
Fragments arranged by size:
3539 1917 563 35 35 35
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DraIII CAC_nnn'GTG
Cuts at: 2931 2989 2931
Size: 58 6066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089
Size: 54 1425 50 275 18 1741 889 1629
Cuts at: 6089 8
Size: 43
Fragments arranged by size:
1741 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2340 2769 3066 3182 4054 1353
Size: 741 246 429 297 116 872 3423
Fragments arranged by size:
3423 872 741 429 297 246 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2126 4711 5085 5231 387
Size: 1064 138 537 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 537 374 146 138
EcoICRI GAG'CTC
Cuts at: 2305 3036 2305
Size: 731 5393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2131 2147 2279 2291 2294 2364 2393
Size: 393 499 16 132 12 3 70 29
Cuts at: 2393 2670 3178 3426 4187 4784 5571 5839 1239
Size: 277 508 248 761 597 787 268 1524
Fragments arranged by size:
1524 787 761 597 508 499 393 277
268 248 132 70 29 16 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2817 2297
Size: 520 5604
EcoO109I rG'GnC_Cy
Cuts at: 2042 2069 2188 2531 2532 2042
Size: 27 119 343 1 5634
Fragments arranged by size:
5634 343 119 27 1
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
EcoRV GAT'ATC
Cuts at: 2448 2448
Size: 6124
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 2146 2343 3080 5271 1678
Size: 468 197 737 2191 2531
Fragments arranged by size:
2531 2191 737 468 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 2432 2464 3181
Size: 32 135 32 816 32 12 32 717
Cuts at: 3181 3213 4765 4797 4839 4871 1373
Size: 32 1552 32 42 32 2626
Fragments arranged by size:
2626 1552 816 717 135 42 32 32
32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3468 3714 669
Size: 988 281 1530 246 3079
Fragments arranged by size:
3079 1530 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2940 3021 748
Size: 2192 81 3851
Fragments arranged by size:
3851 2192 81
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KasI G'GCGC_C
Cuts at: 3076 3076
Size: 6124
KpnI G_GTAC'C
Cuts at: 2164 3449 2164
Size: 1285 4839
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3315 5238 5422 1305
Size: 2010 1923 184 2007
Fragments arranged by size:
2010 2007 1923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4302
Fragments arranged by size:
4302 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3085 3253 3348 4082 4441 4600 518
Size: 648 1919 168 95 734 359 159 2042
Fragments arranged by size:
2042 1919 734 648 359 168 159 95
MspA1I CmG'CkG
Cuts at: 1606 3114 3540 4217 5365 5610 1606
Size: 1508 426 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 426 245
NarI GG'CG_CC
Cuts at: 3077 3077
Size: 6124
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5572
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2488 3206 3482 5027 1132
Size: 97 1259 718 276 1545 2229
Fragments arranged by size:
2229 1545 1259 718 276 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PfoI T'CCnGG_A
Cuts at: 2256 2256
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 2784 2816 4193 4225 5706 5738 2552
Size: 32 200 32 1377 32 1481 32 2938
Fragments arranged by size:
2938 1481 1377 200 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PspOMI G'GGCC_C
Cuts at: 2041 2531 2041
Size: 490 5634
PstI C_TGCA'G
Cuts at: 830 3476 830
Size: 2646 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
SacI G_AGCT'C
Cuts at: 2307 3038 2307
Size: 731 5393
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SapI GCTCTTCn'nnn_
Cuts at: 3182 3182
Size: 6124
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SexAI A'CCwGG_T
Cuts at: 2400 2400
Size: 6124
SfcI C'TryA_G
Cuts at: 826 1045 2974 3364 3472 4607 5288 5479 826
Size: 219 1929 390 108 1135 681 191 1471
Fragments arranged by size:
1929 1471 1135 681 390 219 191 108
SfoI GGC'GCC
Cuts at: 3078 3078
Size: 6124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2574 4228 5129 5391 820
Size: 197 926 631 1654 901 262 1553
Fragments arranged by size:
1654 1553 926 901 631 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 2488 3206 3482 1132
Size: 1356 718 276 3774
Fragments arranged by size:
3774 1356 718 276
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 2050 2312 1517
Size: 533 262 5329
Fragments arranged by size:
5329 533 262
StyI C'CwwG_G
Cuts at: 513 1065 2013 2045 2244 2499 3563 513
Size: 552 948 32 199 255 1064 3074
Fragments arranged by size:
3074 1064 948 552 255 199 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2305 4151 4168 4321 4506 4951 325
Size: 1255 725 1846 17 153 185 445 1498
Fragments arranged by size:
1846 1498 1255 725 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 4370 6082
Size: 274 80 33 51 1443 561 1832 1712
Cuts at: 6082 96
Size: 138
Fragments arranged by size:
1832 1712 1443 561 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6084 6116 6084
Size: 32 6092
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2171
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 2799 2942 3191 3275 4253 2799
Size: 143 249 84 978 4670
Fragments arranged by size:
4670 978 249 143 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI