Restriction map for
pFN21AB8290
(Circular) (Six-base) MAPSORT of: pFN21AB8290.seq Check: 2353 from: 1 to: 6388
pFN21AB8290 6388 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 1937
Size: 1794 4594
Acc65I G'GTAC_C
Cuts at: 3566 3709 3566
Size: 143 6245
AclI AA'CG_TT
Cuts at: 1563 3687 4515 4888 1563
Size: 2124 828 373 3063
Fragments arranged by size:
3063 2124 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2765 3221 3405 4451 5835 6103 1632
Size: 1133 456 184 1046 1384 268 1917
Fragments arranged by size:
1917 1384 1133 1046 456 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 2071 5287 6264 1203
Size: 868 3216 977 1327
Fragments arranged by size:
3216 1327 977 868
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3070 3102 3070
Size: 32 6356
AlwNI CAG_nnn'CTG
Cuts at: 2897 4131 5703 2897
Size: 1234 1572 3582
Fragments arranged by size:
3582 1572 1234
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2039 3697 3923 4075 6184 1493
Size: 546 1658 226 152 2109 1697
Fragments arranged by size:
2109 1697 1658 546 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2767 3713 1870
Size: 73 824 946 4545
Fragments arranged by size:
4545 946 824 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3508 3541 3508
Size: 33 6355
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3307 3566 3709 5164 618
Size: 325 217 627 1520 259 143 1455 1842
Fragments arranged by size:
1842 1520 1455 627 325 259 217 143
BanII G_rGCy'C
Cuts at: 1391 3707 1391
Size: 2316 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2647 2787 2928 928
Size: 971 748 140 141 4388
Fragments arranged by size:
4388 971 748 141 140
BbvCI CC'TCA_GC
Cuts at: 3473 3473
Size: 6388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2692 3187 3529 4280 5490 2692
Size: 495 342 751 1210 3590
Fragments arranged by size:
3590 1210 751 495 342
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3805 3805
Size: 6388
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3318 3516 4119
Size: 416 53 83 399 24 1901 198 603
Cuts at: 4119 5077 442
Size: 958 1753
Fragments arranged by size:
1901 1753 958 603 416 399 198 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3579 1056
Size: 2523 3865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2471 2471
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 2670 2730 3069 3114 5048 1239
Size: 1362 69 60 339 45 1934 2579
Fragments arranged by size:
2579 1934 1362 339 69 60 45
Bpu10I CC'TnA_GC
Cuts at: 3347 3473 3347
Size: 126 6262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 3396 4513 5378 5676 2648
Size: 748 1117 865 298 3360
Fragments arranged by size:
3360 1117 865 748 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2793 3438 5051 882
Size: 617 1294 645 1613 2219
Fragments arranged by size:
2219 1613 1294 645 617
BsaAI yAC'GTr
Cuts at: 493 1204 2554 4179 493
Size: 711 1350 1625 2702
Fragments arranged by size:
2702 1625 1350 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2389 4195 4577 5212
Size: 53 83 186 936 856 1806 382 635
Cuts at: 5212 275
Size: 1451
Fragments arranged by size:
1806 1451 936 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4821 5219 5493 5640 6051 4821
Size: 398 274 147 411 5158
Fragments arranged by size:
5158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3064 3070 3094 3100 1178
Size: 30 165 30 1661 6 24 6 4466
Fragments arranged by size:
4466 1661 165 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6388
BseYI C'CCAG_C
Cuts at: 2482 3024 5591 6087 2482
Size: 542 2567 496 2783
Fragments arranged by size:
2783 2567 542 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 3348 1163
Size: 573 840 772 4203
Fragments arranged by size:
4203 840 772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2286 3017 4449 4534 5605 2286
Size: 731 1432 85 1071 3069
Fragments arranged by size:
3069 1432 1071 731 85
BsmI GAATG_Cn'
Cuts at: 3899 3992 3899
Size: 93 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 3017 3707 4449 4534 5605 1391
Size: 399 496 731 690 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 731 690 496 399 85
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 2457 2755 4277 6007 2457
Size: 298 1522 1730 2838
Fragments arranged by size:
2838 1730 1522 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 3743 844
Size: 1268 1631 3489
Fragments arranged by size:
3489 1631 1268
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 4275 1394
Size: 429 629 1823 3507
Fragments arranged by size:
3507 1823 629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2470 4883 5057 65
Size: 1183 1222 2413 174 1396
Fragments arranged by size:
2413 1396 1222 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3619 5032 5219 1700
Size: 110 1809 1413 187 2869
Fragments arranged by size:
2869 1809 1413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 2621 1184
Size: 1437 4951
BstXI CCAn_nnnn'nTGG
Cuts at: 2708 2708
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 2395 3718 4085 4470 4487 5939 6025 6037
Size: 423 1323 367 385 17 1452 86 12
Cuts at: 6037 6383 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1323 423 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3189 3400 3189
Size: 211 6177
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 3336 3904 4698 4718 3265
Size: 71 568 794 20 4935
Fragments arranged by size:
4935 794 568 71 20
ClaI AT'CG_AT
Cuts at: 1554 3421 1554
Size: 1867 4521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 2131 3692 2131
Size: 1561 4827
DraIII CAC_nnn'GTG
Cuts at: 2492 3062 2492
Size: 570 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 3621 3835
Size: 54 1425 50 275 18 1456 335 214
Cuts at: 3835 4724 6353 8
Size: 889 1629 43
Fragments arranged by size:
1629 1456 1425 889 335 275 214 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4318 1353
Size: 2965 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2601 2670 2730 2765 3069 3114 3221
Size: 393 969 69 60 35 304 45 107
Cuts at: 3221 3405 4451 5048 5835 6103 1239
Size: 184 1046 597 787 268 1524
Fragments arranged by size:
1524 1046 969 787 597 393 304 268
184 107 69 60 45 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2369
Size: 6388
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
EcoRV GAT'ATC
Cuts at: 3170 3170
Size: 6388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2201 3140 2201
Size: 939 5449
FspI TGC'GCA
Cuts at: 2246 4894 2246
Size: 2648 3740
HaeII r_GCGC'y
Cuts at: 1678 5535 1678
Size: 3857 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2750 2782 3481
Size: 32 135 32 427 32 719 32 699
Cuts at: 3481 3513 5029 5061 5103 5135 1373
Size: 32 1516 32 42 32 2626
Fragments arranged by size:
2626 1516 719 699 427 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2716 3732 3978 669
Size: 988 281 778 1016 246 3079
Fragments arranged by size:
3079 1016 988 778 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3570 3713 3570
Size: 143 6245
MfeI C'AATT_G
Cuts at: 2082 2724 3987 2082
Size: 642 1263 4483
Fragments arranged by size:
4483 1263 642
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2966 3134 3182 5502 5686 1305
Size: 1661 168 48 2320 184 2007
Fragments arranged by size:
2320 2007 1661 184 168 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3288 10
Size: 54 1425 343 1456 3110
Fragments arranged by size:
3110 1456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 3324 4346 4705 4864 518
Size: 648 910 1248 1022 359 159 2042
Fragments arranged by size:
2042 1248 1022 910 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2894 3472 3804 4481 5629 5874 1606
Size: 1288 578 332 677 1148 245 2120
Fragments arranged by size:
2120 1288 1148 677 578 332 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5836
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 3575 1052
Size: 2523 3865
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 2075 3746 5291 1132
Size: 97 846 1671 1545 2229
Fragments arranged by size:
2229 1671 1545 846 97
PasI CC'CwG_GG
Cuts at: 1348 2701 1348
Size: 1353 5035
PciI A'CATG_T
Cuts at: 2071 5287 2071
Size: 3216 3172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2897 1108
Size: 1789 4599
PfoI T'CCnGG_A
Cuts at: 3037 3037
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3070 3102 4457 4489 5970 6002 3070
Size: 32 1355 32 1481 32 3456
Fragments arranged by size:
3456 1481 1355 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PstI C_TGCA'G
Cuts at: 830 2109 3740 830
Size: 1279 1631 3478
Fragments arranged by size:
3478 1631 1279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2894 3472 2894
Size: 578 5810
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 2109 3740 2109
Size: 1631 4757
ScaI AGT'ACT
Cuts at: 2126 4636 6348 2126
Size: 2510 1712 2166
Fragments arranged by size:
2510 2166 1712
SfcI C'TryA_G
Cuts at: 826 1045 2105 2859 3736 4871 5552 5743 826
Size: 219 1060 754 877 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 877 754 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 3411 4492 5393 5655 820
Size: 197 926 684 784 1081 901 262 1553
Fragments arranged by size:
1553 1081 926 901 784 684 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2430 3827 513
Size: 552 1365 1397 3074
Fragments arranged by size:
3074 1397 1365 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 3625 4415 4432 4585 4770 5215
Size: 1255 481 1564 790 17 153 185 445
Cuts at: 5215 325
Size: 1498
Fragments arranged by size:
1564 1498 1255 790 481 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 4634 6346
Size: 274 80 33 51 1443 147 2510 1712
Cuts at: 6346 96
Size: 138
Fragments arranged by size:
2510 1712 1443 274 147 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2478 2510 3105 3137 6348 6380 2478
Size: 32 595 32 3211 32 2486
Fragments arranged by size:
3211 2486 595 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2381 1316
Size: 1065 5323
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 3424
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 4517 4517
Size: 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI