Restriction map for
pFN21AB8289
(Circular) (Six-base) MAPSORT of: pFN21AB8289.seq Check: 8076 from: 1 to: 5611
pFN21AB8289 5611 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2649 3674 5058 5326 1632
Size: 1017 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1025 1017 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 2010 4510 5487 1203
Size: 807 2500 977 1327
Fragments arranged by size:
2500 1327 977 807
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 2736 2826 1166
Size: 1570 90 3951
Fragments arranged by size:
3951 1570 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 3354 4926 3354
Size: 1572 4039
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2920 3146 3298 5407 1493
Size: 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 2936 1870
Size: 73 361 632 4545
Fragments arranged by size:
4545 632 361 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2679 2932 4387 618
Size: 325 217 627 892 253 1455 1842
Fragments arranged by size:
1842 1455 892 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2145 2282 2436 2802 2930 1391
Size: 754 137 154 366 128 4072
Fragments arranged by size:
4072 754 366 154 137 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BbvCI CC'TCA_GC
Cuts at: 2333 2333
Size: 5611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2513 2570 4222
Size: 107 122 71 631 605 841 57 1652
Cuts at: 4222 136
Size: 1525
Fragments arranged by size:
1652 1525 841 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 2435 3028 2435
Size: 593 5018
Bme1580I G_kGCm'C
Cuts at: 1790 3672 4828 1790
Size: 1882 1156 2573
Fragments arranged by size:
2573 1882 1156
BmgBI CAC'GTC
Cuts at: 1318 2011 1318
Size: 693 4918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2324 4271 1239
Size: 869 216 1947 2579
Fragments arranged by size:
2579 1947 869 216
Bpu10I CC'TnA_GC
Cuts at: 2333 2430 2333
Size: 97 5514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 2153 2318 4274 882
Size: 617 588 66 165 1956 2219
Fragments arranged by size:
2219 1956 617 588 165 66
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 1186
Size: 919 4692
BseYI C'CCAG_C
Cuts at: 2388 4814 5310 2388
Size: 2426 496 2689
Fragments arranged by size:
2689 2426 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5038
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2282 2436 2759 2802 3672 3757 4828 2282
Size: 154 323 43 870 85 1071 3065
Fragments arranged by size:
3065 1071 870 323 154 85 43
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2282 2436 2759 2802 2930 3672
Size: 399 355 137 154 323 43 128 742
Cuts at: 3672 3757 4828 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 323 154 137
128 85 43
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2336 2966 844
Size: 1492 630 3489
Fragments arranged by size:
3489 1492 630
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2344 4255 4442 1700
Size: 110 250 284 1911 187 2869
Fragments arranged by size:
2869 1911 284 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 2803 3671 4683 2803
Size: 868 1012 3731
Fragments arranged by size:
3731 1012 868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2555 2853 794
Size: 1761 298 3552
Fragments arranged by size:
3552 1761 298
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2151 2941 3308 3693 3710 5162 5248 5260
Size: 179 790 367 385 17 1452 86 12
Cuts at: 5260 5606 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 790 385 367 346 179 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2253 2475 513
Size: 552 939 249 222 3649
Fragments arranged by size:
3649 939 552 249 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2808 487
Size: 683 56 1582 3290
Fragments arranged by size:
3290 1582 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2406 2851 3127 3921 3941 2292
Size: 114 445 276 794 20 3962
Fragments arranged by size:
3962 794 445 276 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2346 2505
Size: 54 1425 50 275 18 432 84 159
Cuts at: 2505 3058 3947 5576 8
Size: 553 889 1629 43
Fragments arranged by size:
1629 1425 889 553 432 275 159 84
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2359 2645 2894 3541 1353
Size: 917 89 286 249 647 3423
Fragments arranged by size:
3423 917 647 286 249 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2280 2434 2800 2280
Size: 154 366 5091
Fragments arranged by size:
5091 366 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2324 2649 3674 4271 5058 5326
Size: 393 476 216 325 1025 597 787 268
Cuts at: 5326 1239
Size: 1524
Fragments arranged by size:
1524 1025 787 597 476 393 325 268
216
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 4758 1678
Size: 3080 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373
Size: 32 135 32 2680 32 42 32 2626
Fragments arranged by size:
2680 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2955 3201 669
Size: 988 281 442 575 246 3079
Fragments arranged by size:
3079 988 575 442 281 246
HindIII A'AGCT_T
Cuts at: 748 2221 748
Size: 1473 4138
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4725 4909 1305
Size: 3420 184 2007
Fragments arranged by size:
3420 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2507 10
Size: 54 1425 343 675 3114
Fragments arranged by size:
3114 1425 675 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2736 2826 3569 3928 4087 518
Size: 648 843 727 90 743 359 159 2042
Fragments arranged by size:
2042 843 743 727 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2555 2664 3027 3704 4852 5097 1606
Size: 949 109 363 677 1148 245 2120
Fragments arranged by size:
2120 1148 949 677 363 245 109
NaeI GCC'GGC
Cuts at: 2346 2346
Size: 5611
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4120
Fragments arranged by size:
4120 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NgoMIV G'CCGG_C
Cuts at: 2344 2344
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2112 2637 2969 4514 1132
Size: 97 883 525 332 1545 2229
Fragments arranged by size:
2229 1545 883 525 332 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PfoI T'CCnGG_A
Cuts at: 2034 2034
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 2751 3181 2751
Size: 430 5181
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2115 2147 2115
Size: 32 5579
PstI C_TGCA'G
Cuts at: 830 2560 2963 830
Size: 1730 403 3478
Fragments arranged by size:
3478 1730 403
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2555 2555
Size: 5611
SacI G_AGCT'C
Cuts at: 2282 2436 2802 2282
Size: 154 366 5091
Fragments arranged by size:
5091 366 154
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2270 2359 2270
Size: 89 5522
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2132 2556 2812 2959 4094 4775 4966
Size: 219 1087 424 256 147 1135 681 191
Cuts at: 4966 826
Size: 1471
Fragments arranged by size:
1471 1135 1087 681 424 256 219 191
147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3715 4616 4878 820
Size: 197 926 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5611
StyI C'CwwG_G
Cuts at: 513 1065 2004 2611 3050 513
Size: 552 939 607 439 3074
Fragments arranged by size:
3074 939 607 552 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 3655 3808 3993 4438 325
Size: 1255 2058 17 153 185 445 1498
Fragments arranged by size:
2058 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3857 5569 96
Size: 274 80 33 51 1443 1880 1712 138
Fragments arranged by size:
1880 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2359 2947 2359
Size: 588 5023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI