Restriction map for
pFN21AB8288
(Circular) (Six-base) MAPSORT of: pFN21AB8288.seq Check: 6861 from: 1 to: 5926
pFN21AB8288 5926 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 3989 5373 5641 1632
Size: 665 1692 1384 268 1917
Fragments arranged by size:
1917 1692 1384 665 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 3033 4655 3033
Size: 1622 4304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2207 1184
Size: 1023 4903
AlwNI CAG_nnn'CTG
Cuts at: 2035 2047 2337 2624 3669 5241 2035
Size: 12 290 287 1045 1572 2720
Fragments arranged by size:
2720 1572 1045 290 287 12
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2099 3235 3461 3613 5722 1493
Size: 606 1136 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2448 4480 160
Size: 872 1416 2032 1606
Fragments arranged by size:
2032 1606 1416 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2971 3251 1870
Size: 73 1028 280 4545
Fragments arranged by size:
4545 1028 280 73
BamHI G'GATC_C
Cuts at: 2284 2975 3256 3623 2284
Size: 691 281 367 4587
Fragments arranged by size:
4587 691 367 281
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2927 3247 4702 618
Size: 325 217 627 1140 320 1455 1842
Fragments arranged by size:
1842 1455 1140 627 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2191 2823 2843 3245 1391
Size: 800 632 20 402 4072
Fragments arranged by size:
4072 800 632 402 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2523 928
Size: 971 624 4331
Fragments arranged by size:
4331 971 624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2917 2951 3022 3056 4117 4151 1903
Size: 34 980 34 71 34 1061 34 3678
Fragments arranged by size:
3678 1061 980 71 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 2550 1548
Size: 1002 4924
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2480 2645 4537
Size: 107 122 71 631 605 808 165 1892
Cuts at: 4537 136
Size: 1525
Fragments arranged by size:
1892 1525 808 631 605 165 122 107
71
BglII A'GATC_T
Cuts at: 2667 5921 2667
Size: 3254 2672
BlpI GC'TnA_GC
Cuts at: 2180 3343 2180
Size: 1163 4763
Bme1580I G_kGCm'C
Cuts at: 1790 3987 5143 1790
Size: 2197 1156 2573
Fragments arranged by size:
2573 2197 1156
BmgBI CAC'GTC
Cuts at: 1318 2584 2616 1318
Size: 1266 32 4628
Fragments arranged by size:
4628 1266 32
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3165 3657 4615
Size: 416 53 83 399 24 1748 492 958
Cuts at: 4615 442
Size: 1753
Fragments arranged by size:
1753 1748 958 492 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 2807 2837 3031 3082 3177 4586 1239
Size: 1283 285 30 194 51 95 1409 2579
Fragments arranged by size:
2579 1409 1283 285 194 95 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2345 2472 2633 3733
Size: 53 83 186 936 812 127 161 1100
Cuts at: 3733 4115 4750 275
Size: 382 635 1451
Fragments arranged by size:
1451 1100 936 812 635 382 186 161
127 83 53
BsaWI w'CCGG_w
Cuts at: 2350 4359 4757 5031 5178 5589 2350
Size: 2009 398 274 147 411 2687
Fragments arranged by size:
2687 2009 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2839 2869 2924 2954 1178
Size: 30 165 30 1436 30 55 30 4150
Fragments arranged by size:
4150 1436 165 55 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2519 2662 1186
Size: 913 420 143 4450
Fragments arranged by size:
4450 913 420 143
BseYI C'CCAG_C
Cuts at: 2959 2986 5129 5625 2959
Size: 27 2143 496 3260
Fragments arranged by size:
3260 2143 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2922 3987 4072 5143 2922
Size: 1065 85 1071 3705
Fragments arranged by size:
3705 1071 1065 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
BsmBI CGTCTCn'nnnn_
Cuts at: 2352 2662 2352
Size: 310 5616
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2823 2843 2922 3245 3987 4072
Size: 399 401 632 20 79 323 742 85
Cuts at: 4072 5143 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 632 401 399 323 85
79 20
BspEI T'CCGG_A
Cuts at: 2350 5589 2350
Size: 3239 2687
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2593 4570 4757 1700
Size: 110 783 1977 187 2869
Fragments arranged by size:
2869 1977 783 187 110
BsrGI T'GTAC_A
Cuts at: 96 2247 96
Size: 2151 3775
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 2398 3986 4998 2398
Size: 1588 1012 3326
Fragments arranged by size:
3326 1588 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 794
Size: 1253 4673
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2893 2596
Size: 297 5629
BstYI r'GATC_y
Cuts at: 1972 2271 2284 2667 2975 3256 3623 4008 4025
Size: 299 13 383 308 281 367 385 17
Cuts at: 4025 5477 5563 5575 5921 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 383 367 346 308 299
281 86 17 13 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2529 513
Size: 552 939 525 3910
Fragments arranged by size:
3910 939 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2929 487
Size: 683 56 1703 3484
Fragments arranged by size:
3484 1703 683 56
BtsI GCAGTG_nn'
Cuts at: 3038 3137 3442 4236 4256 3038
Size: 99 305 794 20 4708
Fragments arranged by size:
4708 794 305 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DraIII CAC_nnn'GTG
Cuts at: 2025 2296 2025
Size: 271 5655
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 4933 809
Size: 507 1266 2351 1802
Fragments arranged by size:
2351 1802 1266 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2382 2661
Size: 54 1425 50 275 18 297 255 279
Cuts at: 2661 3148 3373 4262 5891 8
Size: 487 225 889 1629 43
Fragments arranged by size:
1629 1425 889 487 297 279 275 255
225 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2786 2868 3856 1353
Size: 1433 82 988 3423
Fragments arranged by size:
3423 1433 988 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2626 2698 4513 4887 5033 387
Size: 1064 138 1037 72 1815 374 146 1280
Fragments arranged by size:
1815 1280 1064 1037 374 146 138 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2522 2807 2837 3031 3082 3177
Size: 393 665 225 285 30 194 51 95
Cuts at: 3177 3989 4586 5373 5641 1239
Size: 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 665 597 393 285 268
225 194 95 51 30
EcoRI G'AATT_C
Cuts at: 2099 3235 3613 2099
Size: 1136 378 4412
Fragments arranged by size:
4412 1136 378
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2909 5073 1678
Size: 1231 2164 2531
Fragments arranged by size:
2531 2164 1231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 2839 2871 2924
Size: 32 135 32 700 32 535 32 53
Cuts at: 2924 2956 3019 3051 4567 4599 4641 4673 1373
Size: 32 63 32 1516 32 42 32 2626
Fragments arranged by size:
2626 1516 700 535 135 63 53 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 3150 10
Size: 54 1425 343 297 1021 2786
Fragments arranged by size:
2786 1425 1021 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3158 3884 4243 4402 518
Size: 648 1992 726 359 159 2042
Fragments arranged by size:
2042 1992 726 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2914 3088 3342 4019 5167 5412 1606
Size: 1308 174 254 677 1148 245 2120
Fragments arranged by size:
2120 1308 1148 677 254 245 174
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4435
Fragments arranged by size:
4435 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3157 3284 4829 1132
Size: 97 1928 127 1545 2229
Fragments arranged by size:
2229 1928 1545 127 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2136 1108
Size: 1028 4898
PfoI T'CCnGG_A
Cuts at: 2265 2784 2265
Size: 519 5407
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2328 2460 3278 830
Size: 1498 132 818 3478
Fragments arranged by size:
3478 1498 818 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 3198 4174 5886 3198
Size: 976 1712 3238
Fragments arranged by size:
3238 1712 976
SfcI C'TryA_G
Cuts at: 826 1045 2324 2456 3274 4409 5090 5281 826
Size: 219 1279 132 818 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 818 681 219 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 2427 1517
Size: 910 5016
StyI C'CwwG_G
Cuts at: 513 1065 2004 3365 513
Size: 552 939 1361 3074
Fragments arranged by size:
3074 1361 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2718 3953 3970 4123 4308 4753 325
Size: 1255 1138 1235 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1235 1138 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2430 3196
Size: 274 80 33 51 1443 270 183 766
Cuts at: 3196 4172 5884 96
Size: 976 1712 138
Fragments arranged by size:
1712 1443 976 766 274 270 183 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 2851 1316
Size: 1535 4391
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI