Restriction map for
pFN21AB8287
(Circular) (Six-base) MAPSORT of: pFN21AB8287.seq Check: 3094 from: 1 to: 5971
pFN21AB8287 5971 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2199 2243 2199
Size: 44 5927
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 3314 1937
Size: 966 411 4594
Fragments arranged by size:
4594 966 411
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3043 4034 5418 5686 1632
Size: 1411 991 1384 268 1917
Fragments arranged by size:
1917 1411 1384 991 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 2898 4802 2898
Size: 1904 4067
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2212 3714 5286 2212
Size: 1502 1572 2897
Fragments arranged by size:
2897 1572 1502
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 3296 1870
Size: 73 127 1226 4545
Fragments arranged by size:
4545 1226 127 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3190 3223 3190
Size: 33 5938
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2735 2989 3290 1391
Size: 1344 254 301 4072
Fragments arranged by size:
4072 1344 301 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BfrBI ATG'CAT
Cuts at: 2708 2708
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 4032 5188 1790
Size: 2242 1156 2573
Fragments arranged by size:
2573 2242 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 3121 3195
Size: 416 53 83 399 24 1694 10 74
Cuts at: 3195 3702 4660 442
Size: 507 958 1753
Fragments arranged by size:
1753 1694 958 507 416 399 83 74
53 24 10
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2754 2754
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2497 2871 2955 3039 4631 1239
Size: 911 347 374 84 84 1592 2579
Fragments arranged by size:
2579 1592 911 374 347 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2341 2810 4096 4961 5259 2341
Size: 469 1286 865 298 3053
Fragments arranged by size:
3053 1286 865 469 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 2444 4634 882
Size: 617 627 318 2190 2219
Fragments arranged by size:
2219 2190 627 617 318
BsaAI yAC'GTr
Cuts at: 493 1204 3156 3762 493
Size: 711 1952 606 2702
Fragments arranged by size:
2702 1952 711 606
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2583 3778 4160 4795
Size: 53 83 186 936 1050 1195 382 635
Cuts at: 4795 275
Size: 1451
Fragments arranged by size:
1451 1195 1050 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2428 2898 3147 4404 4802 5076 5223 5634 2428
Size: 470 249 1257 398 274 147 411 2765
Fragments arranged by size:
2765 1257 470 411 398 274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 1186
Size: 904 5067
BseYI C'CCAG_C
Cuts at: 5174 5670 5174
Size: 496 5475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 2492 1163
Size: 573 535 221 4642
Fragments arranged by size:
4642 573 535 221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2266 2735 3155 4032 4117 5188 2266
Size: 469 420 877 85 1071 3049
Fragments arranged by size:
3049 1071 877 469 420 85
BsmI GAATG_Cn'
Cuts at: 2705 3125 3482 3575 2705
Size: 420 357 93 5101
Fragments arranged by size:
5101 420 357 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2006 2006
Size: 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2735 2989 3155 3290 4032 4117
Size: 399 476 469 254 166 135 742 85
Cuts at: 4117 5188 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 476 469 399 254 166
135 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2243 2805 3225 3326 844
Size: 1355 44 562 420 101 3489
Fragments arranged by size:
3489 1355 562 420 101 44
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 3858 1394
Size: 429 491 1544 3507
Fragments arranged by size:
3507 1544 491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 4466 4640 65
Size: 1183 1462 1756 174 1396
Fragments arranged by size:
1756 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2898 4615 4802 1700
Size: 110 1088 1717 187 2869
Fragments arranged by size:
2869 1717 1088 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 2038 1184
Size: 854 5117
BstYI r'GATC_y
Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966
Size: 1329 367 385 17 1452 86 12 346
Cuts at: 5966 1972
Size: 1977
Fragments arranged by size:
1977 1452 1329 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2630 3487 4281 4301 2630
Size: 857 794 20 4300
Fragments arranged by size:
4300 857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2608 2643 2692 2727 529
Size: 35 2044 35 49 35 3773
Fragments arranged by size:
3773 2044 49 35 35 35
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DraIII CAC_nnn'GTG
Cuts at: 3129 3129
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3901 1353
Size: 866 1682 3423
Fragments arranged by size:
3423 1682 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4558 4932 5078 387
Size: 1064 138 435 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2733 2733
Size: 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2497 2871 2955 3039 3043 4034
Size: 393 518 347 374 84 84 4 991
Cuts at: 4034 4631 5418 5686 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 991 787 597 518 393 374 347
268 84 84 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 2444
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 2106 2925 2106
Size: 819 5152
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2489 2489
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2519 2551 4612 4644 4686
Size: 32 135 32 947 32 2061 32 42
Cuts at: 4686 4718 1373
Size: 32 2626
Fragments arranged by size:
2626 2061 947 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2556 2684 2782 3929 4288 4447 518
Size: 648 1390 128 98 1147 359 159 2042
Fragments arranged by size:
2042 1390 1147 648 359 159 128 98
MspA1I CmG'CkG
Cuts at: 1606 2089 3091 3387 4064 5212 5457 1606
Size: 483 1002 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 1002 677 483 296 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NsiI A_TGCA'T
Cuts at: 2710 2710
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2243 2712 3048 3329 4874 1132
Size: 97 1014 469 336 281 1545 2229
Fragments arranged by size:
2229 1545 1014 469 336 281 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2253 2285 4040 4072 5553 5585 2253
Size: 32 1755 32 1481 32 2639
Fragments arranged by size:
2639 1755 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2925 2106
Size: 819 5152
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3252 3323 830
Size: 2422 71 3478
Fragments arranged by size:
3478 2422 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2089 2089
Size: 5971
SacI G_AGCT'C
Cuts at: 2735 2735
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 3248 3319 4454 5135 5326 826
Size: 219 2203 71 1135 681 191 1471
Fragments arranged by size:
2203 1471 1135 681 219 191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 2072 3298 1872
Size: 200 1226 4545
Fragments arranged by size:
4545 1226 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2356 2789 4075 4976 5238 820
Size: 197 926 413 433 1286 901 262 1553
Fragments arranged by size:
1553 1286 926 901 433 413 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 2232 2804 2888 3056 3224 1517
Size: 715 572 84 168 168 4264
Fragments arranged by size:
4264 715 572 168 168 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3410 513
Size: 552 939 1406 3074
Fragments arranged by size:
3074 1406 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 3140 3998 4015 4168 4353 4798
Size: 1255 1329 231 858 17 153 185 445
Cuts at: 4798 325
Size: 1498
Fragments arranged by size:
1498 1329 1255 858 445 231 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2179
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 2070 3296 1870
Size: 200 1226 4545
Fragments arranged by size:
4545 1226 200
XmnI GAAnn'nnTTC
Cuts at: 2804 3224 4100 2804
Size: 420 876 4675
Fragments arranged by size:
4675 876 420
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI