Restriction map for
pFN21AB8285
(Circular) (Six-base) MAPSORT of: pFN21AB8285.seq Check: 6180 from: 1 to: 5887
pFN21AB8285 5887 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 2785 3950 5334 5602 1632
Size: 843 310 1165 1384 268 1917
Fragments arranged by size:
1917 1384 1165 843 310 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 2378 2448 2498 1166
Size: 1212 70 50 4555
Fragments arranged by size:
4555 1212 70 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3630 5202 3630
Size: 1572 4315
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2654 2945 2963 3196 3422 3574 5683 1493
Size: 1161 291 18 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 291 233 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2087 2120 2087
Size: 33 5854
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3208 4663 618
Size: 325 217 627 1421 1455 1842
Fragments arranged by size:
1842 1455 1421 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3206 1391
Size: 1815 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BbvCI CC'TCA_GC
Cuts at: 2043 2043
Size: 5887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2950 3618 4576
Size: 416 53 83 399 24 1533 668 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1533 958 668 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3062 4547 1239
Size: 1823 1485 2579
Fragments arranged by size:
2579 1823 1485
Bpu10I CC'TnA_GC
Cuts at: 2043 2125 2394 2698 2043
Size: 82 269 304 5232
Fragments arranged by size:
5232 304 269 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 4012 4877 5175 2288
Size: 1724 865 298 3000
Fragments arranged by size:
3000 1724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2745 4550 882
Size: 617 1246 1805 2219
Fragments arranged by size:
2219 1805 1246 617
BsaAI yAC'GTr
Cuts at: 493 1204 2792 3678 493
Size: 711 1588 886 2702
Fragments arranged by size:
2702 1588 886 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3065 1186
Size: 1879 4008
BseYI C'CCAG_C
Cuts at: 3179 5090 5586 3179
Size: 1911 496 3480
Fragments arranged by size:
3480 1911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2524 1163
Size: 573 788 4526
Fragments arranged by size:
4526 788 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3148 3398 3491 3148
Size: 250 93 5544
Fragments arranged by size:
5544 250 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3206 3948 4033 5104 1391
Size: 399 1416 742 85 1071 2174
Fragments arranged by size:
2174 1416 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3143 794
Size: 2349 3538
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2348 2173
Size: 175 5712
BstYI r'GATC_y
Cuts at: 1972 3217 3584 3969 3986 5438 5524 5536 5882
Size: 1245 367 385 17 1452 86 12 346
Cuts at: 5882 1972
Size: 1977
Fragments arranged by size:
1977 1452 1245 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2068 2341 487
Size: 683 56 842 273 4033
Fragments arranged by size:
4033 842 683 273 56
BtsI GCAGTG_nn'
Cuts at: 3403 4197 4217 3403
Size: 794 20 5073
Fragments arranged by size:
5073 794 20
ClaI AT'CG_AT
Cuts at: 1554 2589 1554
Size: 1035 4852
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 2962 3191 2962
Size: 229 5658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2336 4894 809
Size: 507 1020 2558 1802
Fragments arranged by size:
2558 1802 1020 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852
Size: 54 1425 50 275 18 1504 889 1629
Cuts at: 5852 8
Size: 43
Fragments arranged by size:
1629 1504 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 3817 1353
Size: 923 1541 3423
Fragments arranged by size:
3423 1541 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2475 2785 3062 3950 4547 5334 5602
Size: 393 843 310 277 888 597 787 268
Cuts at: 5602 1239
Size: 1524
Fragments arranged by size:
1524 888 843 787 597 393 310 277
268
EcoO109I rG'GnC_Cy
Cuts at: 2047 2400 3070 2047
Size: 353 670 4864
Fragments arranged by size:
4864 670 353
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373
Size: 32 135 32 2956 32 42 32 2626
Fragments arranged by size:
2956 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4065
Fragments arranged by size:
4065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2346 2378 2448 2498 3845 4204
Size: 648 1005 175 32 70 50 1347 359
Cuts at: 4204 4363 518
Size: 159 2042
Fragments arranged by size:
2042 1347 1005 648 359 175 159 70
50 32
MspA1I CmG'CkG
Cuts at: 1606 3303 3980 5128 5373 1606
Size: 1697 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5335
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2585 3245 4790 1132
Size: 97 1356 660 1545 2229
Fragments arranged by size:
2229 1545 1356 660 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PfoI T'CCnGG_A
Cuts at: 3051 3051
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PmlI CAC'GTG
Cuts at: 2792 2792
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2400 2047
Size: 353 5534
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 3139 3239 830
Size: 2309 100 3478
Fragments arranged by size:
3478 2309 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SanDI GG'GwC_CC
Cuts at: 2400 2400
Size: 5887
SapI GCTCTTCn'nnn_
Cuts at: 2276 2276
Size: 5887
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 5887
SfcI C'TryA_G
Cuts at: 826 1045 3135 3235 4370 5051 5242 826
Size: 219 2090 100 1135 681 191 1471
Fragments arranged by size:
2090 1471 1135 681 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 3991 4892 5154 820
Size: 197 926 360 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 360 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 3125 1517
Size: 1608 4279
StyI C'CwwG_G
Cuts at: 513 1065 2106 2332 2715 3326 513
Size: 552 1041 226 383 611 3074
Fragments arranged by size:
3074 1041 611 552 383 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2091 2867 3914 3931 4084 4269 4714
Size: 1255 511 776 1047 17 153 185 445
Cuts at: 4714 325
Size: 1498
Fragments arranged by size:
1498 1255 1047 776 511 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2992 3024 5847 5879 2992
Size: 32 2823 32 3000
Fragments arranged by size:
3000 2823 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2051 1316
Size: 735 5152
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2068
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2192 4016 2192
Size: 1824 4063
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SfiI SfoI SrfI SwaI