Restriction map for
pFN21AB8282
(Circular) (Six-base) MAPSORT of: pFN21AB8282.seq Check: 7291 from: 1 to: 5629
pFN21AB8282 5629 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2037 3439 278
Size: 53 83 186 936 501 1402 2468
Fragments arranged by size:
2468 1402 936 501 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 1937
Size: 1035 4594
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2393 2544 3692 5076 5344 1632
Size: 761 151 1148 1384 268 1917
Fragments arranged by size:
1917 1384 1148 761 268 151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 3372 4944 3372
Size: 1572 4057
ApaI G_GGCC'C
Cuts at: 2838 2838
Size: 5629
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2423 2954 1870
Size: 73 480 531 4545
Fragments arranged by size:
4545 531 480 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2720 2950 4405 618
Size: 325 217 627 933 230 1455 1842
Fragments arranged by size:
1842 1455 933 627 325 230 217
BanII G_rGCy'C
Cuts at: 1391 2838 2948 1391
Size: 1447 110 4072
Fragments arranged by size:
4072 1447 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2550 2678 928
Size: 971 651 128 3879
Fragments arranged by size:
3879 971 651 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3820 3854 1903
Size: 34 50 34 1799 34 3678
Fragments arranged by size:
3678 1799 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2402 2838 3690 4846 1790
Size: 612 436 852 1156 2573
Fragments arranged by size:
2573 1156 852 612 436
BmgBI CAC'GTC
Cuts at: 1318 2107 2166 1318
Size: 789 59 4781
Fragments arranged by size:
4781 789 59
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4289 1239
Size: 3050 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 3754 4619 4917 2699
Size: 1055 865 298 3411
Fragments arranged by size:
3411 1055 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2890 4292 882
Size: 617 1391 1402 2219
Fragments arranged by size:
2219 1402 1391 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 3436 3818 4453
Size: 53 83 186 936 501 1402 382 635
Cuts at: 4453 275
Size: 1451
Fragments arranged by size:
1451 1402 936 635 501 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 1178
Size: 30 165 30 607 30 4767
Fragments arranged by size:
4767 607 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2083 2837 1186
Size: 894 3 754 3978
Fragments arranged by size:
3978 894 754 3
BseYI C'CCAG_C
Cuts at: 4832 5328 4832
Size: 496 5133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5056
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2535 3690 3775 4846 2535
Size: 1155 85 1071 3318
Fragments arranged by size:
3318 1155 1071 85
BsmI GAATG_Cn'
Cuts at: 2630 3140 3233 2630
Size: 510 93 5026
Fragments arranged by size:
5026 510 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2744 2744
Size: 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2402 2535 2838 2948 3690 3775 4846
Size: 399 612 133 303 110 742 85 1071
Cuts at: 4846 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 612 399 303 133 110
85
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 2160 3518 5248 2160
Size: 1358 1730 2541
Fragments arranged by size:
2541 1730 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 844
Size: 2140 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2794 3516 1394
Size: 429 971 722 3507
Fragments arranged by size:
3507 971 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 2428 1925
Size: 503 5126
BssSI C'ACGA_G
Cuts at: 2893 3689 4701 2893
Size: 796 1012 3821
Fragments arranged by size:
3821 1012 796
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2448 794
Size: 1654 3975
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624
Size: 987 367 385 17 1452 86 12 346
Cuts at: 5624 1972
Size: 1977
Fragments arranged by size:
1977 1452 987 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2486 513
Size: 552 1421 3656
Fragments arranged by size:
3656 1421 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2818 487
Size: 683 56 1592 3298
Fragments arranged by size:
3298 1592 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 2479 2621 2635 2703 3145 3939 3959 2437
Size: 42 142 14 68 442 794 20 4107
Fragments arranged by size:
4107 794 442 142 68 42 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 3076 3965
Size: 54 1425 50 275 18 441 805 889
Cuts at: 3965 5594 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 805 441 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2083 2540 3559 1353
Size: 730 457 1019 3423
Fragments arranged by size:
3423 1019 730 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2544 3692 4289 5076 5344 1239
Size: 393 761 151 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 787 761 597 393 268 151
EcoO109I rG'GnC_Cy
Cuts at: 2834 2835 2834
Size: 1 5628
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 5629
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 4776 1678
Size: 3098 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2066 2098 4270
Size: 32 135 32 438 32 24 32 2172
Cuts at: 4270 4302 4344 4376 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2172 438 135 42 32 32 32
32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2248 2973 3219 669
Size: 988 281 310 725 246 3079
Fragments arranged by size:
3079 988 725 310 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 2497 3228 2497
Size: 731 4898
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3807
Fragments arranged by size:
3807 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3587 3946 4105 518
Size: 648 2421 359 159 2042
Fragments arranged by size:
2421 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2371 3045 3722 4870 5115 1606
Size: 765 674 677 1148 245 2120
Fragments arranged by size:
2120 1148 765 677 674 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5077
NdeI CA'TA_TG
Cuts at: 387 2738 387
Size: 2351 3278
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2987 4532 1132
Size: 97 1758 1545 2229
Fragments arranged by size:
2229 1758 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2318 1348
Size: 970 4659
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PfoI T'CCnGG_A
Cuts at: 2028 2028
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2204 1613
Size: 591 5038
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2834 2834
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2444 2710 2981 830
Size: 1614 266 271 3478
Fragments arranged by size:
3478 1614 271 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2371 2371
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SapI GCTCTTCn'nnn_
Cuts at: 2540 2540
Size: 5629
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2440 2706 2977 4112 4793 4984 826
Size: 219 1395 266 271 1135 681 191 1471
Fragments arranged by size:
1471 1395 1135 681 271 266 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 3733 4634 4896 820
Size: 197 926 735 1055 901 262 1553
Fragments arranged by size:
1553 1055 926 901 735 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5629
StyI C'CwwG_G
Cuts at: 513 1065 3068 513
Size: 552 2003 3074
Fragments arranged by size:
3074 2003 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2479 2738 3656 3673 3826 4011 4456
Size: 1255 899 259 918 17 153 185 445
Cuts at: 4456 325
Size: 1498
Fragments arranged by size:
1498 1255 918 899 445 259 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 2117 1316
Size: 801 4828
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2035 3437 276
Size: 53 83 186 936 501 1402 2468
Fragments arranged by size:
2468 1402 936 501 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI