Restriction map for
pFN21AB8279
(Circular) (Six-base) MAPSORT of: pFN21AB8279.seq Check: 1187 from: 1 to: 5923
pFN21AB8279 5923 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2548 3266 1937
Size: 611 718 4594
Fragments arranged by size:
4594 718 611
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 2459 2462 2815 2899 3986 5370 5638
Size: 801 26 3 353 84 1087 1384 268
Cuts at: 5638 1632
Size: 1917
Fragments arranged by size:
1917 1384 1087 801 353 268 84 26
3
AfeI AGC'GCT
Cuts at: 3053 3053
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 2033 3185 820
Size: 197 1016 1152 3558
Fragments arranged by size:
3558 1152 1016 197
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 2157 4652 2157
Size: 2495 3428
AleI CACnn'nnGTG
Cuts at: 1166 3126 1166
Size: 1960 3963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2163 3248 1870
Size: 73 220 1085 4545
Fragments arranged by size:
4545 1085 220 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 4699 618
Size: 325 217 627 1457 1455 1842
Fragments arranged by size:
1842 1457 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2289 3242 1391
Size: 898 953 4072
Fragments arranged by size:
4072 953 898
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 928
Size: 971 162 4790
Fragments arranged by size:
4790 971 162
BbvCI CC'TCA_GC
Cuts at: 3005 3005
Size: 5923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3027 3815 5025 3027
Size: 788 1210 3925
Fragments arranged by size:
3925 1210 788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2350 5918 2350
Size: 3568 2355
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 3984 5140 1790
Size: 2194 1156 2573
Fragments arranged by size:
2573 2194 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2799 3654 4612
Size: 416 53 83 399 24 1382 855 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1382 958 855 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2276 2373 2727 2895 3063 3231 4583
Size: 1034 3 97 354 168 168 168 1352
Cuts at: 4583 1239
Size: 2579
Fragments arranged by size:
2579 1352 1034 354 168 168 168 97
3
Bpu10I CC'TnA_GC
Cuts at: 2207 3005 2207
Size: 798 5125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3211 4586 882
Size: 617 1712 1375 2219
Fragments arranged by size:
2219 1712 1375 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 2503 5917 2503
Size: 3414 2509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2716 3730 4112 4747
Size: 53 83 186 936 1183 1014 382 635
Cuts at: 4747 275
Size: 1451
Fragments arranged by size:
1451 1183 1014 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2314 4356 4754 5028 5175 5586 2314
Size: 2042 398 274 147 411 2651
Fragments arranged by size:
2651 2042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2342 2993 1186
Size: 1111 45 651 4116
Fragments arranged by size:
4116 1111 651 45
BseYI C'CCAG_C
Cuts at: 5126 5622 5126
Size: 496 5427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3166 3175 1163
Size: 573 1430 9 3911
Fragments arranged by size:
3911 1430 573 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2289 2310 2421 2775 3984 4069 5140 2289
Size: 21 111 354 1209 85 1071 3072
Fragments arranged by size:
3072 1209 1071 354 111 85 21
BsmI GAATG_Cn'
Cuts at: 2981 3097 3434 3527 2981
Size: 116 337 93 5377
Fragments arranged by size:
5377 337 116 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2707 2707
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2310 2421 2775 3242 3984 4069
Size: 399 499 21 111 354 467 742 85
Cuts at: 4069 5140 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 499 467 399 354 111
85 21
BspEI T'CCGG_A
Cuts at: 2314 5586 2314
Size: 3272 2651
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2734 2932 4418 4592 65
Size: 1183 1486 198 1486 174 1396
Fragments arranged by size:
1486 1486 1396 1183 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 4567 4754 1700
Size: 110 368 2389 187 2869
Fragments arranged by size:
2869 2389 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3153 794
Size: 2359 3564
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2402 2402
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2350 3253 3620 4005 4022 5474 5560 5572
Size: 378 903 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 903 385 378 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2549 2549
Size: 5923
Bsu36I CC'TnA_GG
Cuts at: 2280 2280
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4432
Fragments arranged by size:
4432 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2716 2751 2968 3003 529
Size: 35 2152 35 217 35 3449
Fragments arranged by size:
3449 2152 217 35 35 35
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 3370 4259
Size: 54 1425 50 275 18 274 1266 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1266 889 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2878 4510 4884 5030 387
Size: 1064 138 1289 1632 374 146 1280
Fragments arranged by size:
1632 1289 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2287 2287
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2276 2373 2433 2459 2462 2727
Size: 393 641 3 97 60 26 3 265
Cuts at: 2727 2815 2895 2899 3063 3231 3986 4583 5370
Size: 88 80 4 164 168 755 597 787
Cuts at: 5370 5638 1239
Size: 268 1524
Fragments arranged by size:
1524 787 755 641 597 393 268 265
168 164 97 88 80 60 26 4
3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2279
Size: 5923
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 3055 5070 1678
Size: 1377 2015 2531
Fragments arranged by size:
2531 2015 1377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 2555 2587 4564
Size: 32 135 32 571 32 380 32 1977
Cuts at: 4564 4596 4638 4670 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1977 571 380 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2743 748
Size: 1995 3928
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 10
Size: 54 1425 343 274 3827
Fragments arranged by size:
3827 1425 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2400 2806 2974 3126 3881 4240 4399
Size: 648 1234 406 168 152 755 359 159
Cuts at: 4399 518
Size: 2042
Fragments arranged by size:
2042 1234 755 648 406 359 168 159
152
MspA1I CmG'CkG
Cuts at: 1606 3339 4016 5164 5409 1606
Size: 1733 677 1148 245 2120
Fragments arranged by size:
2120 1733 1148 677 245
NaeI GCC'GGC
Cuts at: 2180 2180
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4432
Fragments arranged by size:
4432 939 552
NdeI CA'TA_TG
Cuts at: 387 2803 2971 387
Size: 2416 168 3339
Fragments arranged by size:
3339 2416 168
NgoMIV G'CCGG_C
Cuts at: 2178 2178
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2566 1108
Size: 1458 4465
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SacI G_AGCT'C
Cuts at: 2289 2289
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 2763 4171 5883 2763
Size: 1408 1712 2803
Fragments arranged by size:
2803 1712 1408
SfcI C'TryA_G
Cuts at: 826 1045 2340 3271 4406 5087 5278 826
Size: 219 1295 931 1135 681 191 1471
Fragments arranged by size:
1471 1295 1135 931 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 3185 4027 4928 5190 820
Size: 197 926 90 1152 842 901 262 1553
Fragments arranged by size:
1553 1152 926 901 842 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 2294 2828 1517
Size: 777 534 4612
Fragments arranged by size:
4612 777 534
StyI C'CwwG_G
Cuts at: 513 1065 2004 3362 513
Size: 552 939 1358 3074
Fragments arranged by size:
3074 1358 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 3950 3967 4120 4305 4750 325
Size: 1255 707 1663 17 153 185 445 1498
Fragments arranged by size:
1663 1498 1255 707 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 2761 4169
Size: 274 80 33 51 1443 672 112 1408
Cuts at: 4169 5881 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1408 672 274 138 112 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2153 2318 2153
Size: 165 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 2744 2828 2912 2996 4052 2744
Size: 84 84 84 1056 4615
Fragments arranged by size:
4615 1056 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI DraIII EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI