Restriction map for
pFN21AB8277
(Circular) (Six-base) MAPSORT of: pFN21AB8277.seq Check: 7500 from: 1 to: 5776
pFN21AB8277 5776 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2383 2383
Size: 5776
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2063 2496 3119 1937
Size: 126 433 623 4594
Fragments arranged by size:
4594 623 433 126
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 3839 5223 5491 1632
Size: 707 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 707 268
AfeI AGC'GCT
Cuts at: 3048 3048
Size: 5776
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2775 2955 4675 5652 1203
Size: 1572 180 1720 977 1327
Fragments arranged by size:
1720 1572 1327 977 180
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2670 2671 2702 2703 2670
Size: 1 31 1 5743
Fragments arranged by size:
5743 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2476 3519 5091 2476
Size: 1043 1572 3161
Fragments arranged by size:
3161 1572 1043
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2975 3085 3311 3463 5572 1493
Size: 1482 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 1482 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3101 1870
Size: 73 700 458 4545
Fragments arranged by size:
4545 700 458 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2743 2776 2743
Size: 33 5743
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3095 1391
Size: 1704 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2375 928
Size: 971 476 4329
Fragments arranged by size:
4329 971 476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 2104 2922 1548
Size: 556 818 4402
Fragments arranged by size:
4402 818 556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 2090 2163 3193 2090
Size: 73 1030 4673
Fragments arranged by size:
4673 1030 73
Bme1580I G_kGCm'C
Cuts at: 1790 2727 3837 4993 1790
Size: 937 1110 1156 2573
Fragments arranged by size:
2573 1156 1110 937
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2021 1056
Size: 965 4811
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2443 2543 2682 2717 4436 1239
Size: 1124 80 100 139 35 1719 2579
Fragments arranged by size:
2579 1719 1124 139 100 80 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2802 2941 4439 882
Size: 617 1303 139 1498 2219
Fragments arranged by size:
2219 1498 1303 617 139
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 2702 2927 5770 2702
Size: 225 2843 2708
Fragments arranged by size:
2843 2708 225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3060 3090 1178
Size: 30 165 30 1657 30 3864
Fragments arranged by size:
3864 1657 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 2036 2419 4979 5475 2036
Size: 383 2560 496 2337
Fragments arranged by size:
2560 2337 496 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3837 3922 4993 3837
Size: 85 1071 4620
Fragments arranged by size:
4620 1071 85
BsmI GAATG_Cn'
Cuts at: 2844 3287 3380 2844
Size: 443 93 5240
Fragments arranged by size:
5240 443 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2025 2339 2025
Size: 314 5462
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2727 3095 3837 3922 4993 1391
Size: 399 937 368 742 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 399 368 85
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 2706 3665 5395 2706
Size: 959 1730 3087
Fragments arranged by size:
3087 1730 959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2383 3131 844
Size: 1539 748 3489
Fragments arranged by size:
3489 1539 748
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 2716 3663 1394
Size: 429 344 549 947 3507
Fragments arranged by size:
3507 947 549 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 2257 4271 4445 65
Size: 1183 1009 2014 174 1396
Fragments arranged by size:
2014 1396 1183 1009 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 2508 96
Size: 2412 3364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 2218 1184
Size: 1034 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 2209
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2647 3106 3473 3858 3875 5327 5413 5425
Size: 675 459 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 675 459 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3292 4086 4106 2950
Size: 342 794 20 4620
Fragments arranged by size:
4620 794 342 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 3223 4112
Size: 54 1425 50 275 18 505 888 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 888 505 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2587 2663 3706 1353
Size: 1234 76 1043 3423
Fragments arranged by size:
3423 1234 1043 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2363 2443 2543 2682 2717 3839
Size: 393 707 24 80 100 139 35 1122
Cuts at: 3839 4436 5223 5491 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1122 787 707 597 393 268 139
100 80 35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2917 2917
Size: 5776
EcoRI G'AATT_C
Cuts at: 2975 3085 3463 2975
Size: 110 378 5288
Fragments arranged by size:
5288 378 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2110 2344 2068
Size: 42 234 5500
Fragments arranged by size:
5500 234 42
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 3050 4923 1678
Size: 1372 1873 2531
Fragments arranged by size:
2531 1873 1372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2362 2394 2420 2452 2901
Size: 32 135 32 790 32 26 32 449
Cuts at: 2901 2933 4417 4449 4491 4523 1373
Size: 32 1484 32 42 32 2626
Fragments arranged by size:
2626 1484 790 449 135 42 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 3120 3366 669
Size: 988 281 292 890 246 3079
Fragments arranged by size:
3079 988 890 292 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 748
Size: 1655 4121
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 4890 5074 1305
Size: 1455 2130 184 2007
Fragments arranged by size:
2130 2007 1455 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2337 10
Size: 54 1425 343 505 3449
Fragments arranged by size:
3449 1425 505 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3192 3869 5017 5262 1606
Size: 1586 677 1148 245 2120
Fragments arranged by size:
2120 1586 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
NdeI CA'TA_TG
Cuts at: 387 2128 387
Size: 1741 4035
NheI G'CTAG_C
Cuts at: 1052 2017 1052
Size: 965 4811
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2779 2959 3134 4679 1132
Size: 97 1550 180 175 1545 2229
Fragments arranged by size:
2229 1550 1545 180 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 2775 2955 4675 2775
Size: 180 1720 3876
Fragments arranged by size:
3876 1720 180
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 2917
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2481 3128 830
Size: 1651 647 3478
Fragments arranged by size:
3478 1651 647
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2587 2587
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SexAI A'CCwGG_T
Cuts at: 2832 2832
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2477 3124 4259 4940 5131 826
Size: 219 1432 647 1135 681 191 1471
Fragments arranged by size:
1471 1432 1135 681 647 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 3215 513
Size: 552 939 1211 3074
Fragments arranged by size:
3074 1211 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2726 3803 3820 3973 4158 4603 325
Size: 1255 1146 1077 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1146 1077 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 3003 4022
Size: 274 80 33 51 1443 531 495 1019
Cuts at: 4022 5734 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 531 495 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2416
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI