Restriction map for
pFN21AB8275
(Circular) (Six-base) MAPSORT of: pFN21AB8275.seq Check: 9912 from: 1 to: 5623
pFN21AB8275 5623 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3433 278
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2376 2966 1937
Size: 439 590 4594
Fragments arranged by size:
4594 590 439
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 3750 4123 1563
Size: 2187 373 3063
Fragments arranged by size:
3063 2187 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2363 2408 2607 3686 5070 5338 1632
Size: 654 77 45 199 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1079 654 268 199 77 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 4352 4352
Size: 5623
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5623
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2003 2035 2003
Size: 32 5591
AlwNI CAG_nnn'CTG
Cuts at: 3366 4938 3366
Size: 1572 4051
ApaLI G'TGCA_C
Cuts at: 3680 4836 3680
Size: 1156 4467
ApoI r'AATT_y
Cuts at: 1493 2360 2553 2932 3158 3310 5419 1493
Size: 867 193 379 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 379 226 193 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2948 1870
Size: 73 1005 4545
Fragments arranged by size:
4545 1005 73
BamHI G'GATC_C
Cuts at: 2953 3320 2953
Size: 367 5256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2944 4399 618
Size: 325 217 627 381 776 1455 1842
Fragments arranged by size:
1842 1455 776 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2643 2842 2942 1391
Size: 1252 199 100 4072
Fragments arranged by size:
4072 1252 199 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2893 3515 4725 2893
Size: 622 1210 3791
Fragments arranged by size:
3791 1210 622
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2096 4234 136
Size: 107 122 71 631 605 424 2138 1525
Fragments arranged by size:
2138 1525 631 605 424 122 107 71
BglII A'GATC_T
Cuts at: 2338 5618 2338
Size: 3280 2343
BlpI GC'TnA_GC
Cuts at: 3040 3040
Size: 5623
Bme1580I G_kGCm'C
Cuts at: 1790 3684 4840 1790
Size: 1894 1156 2573
Fragments arranged by size:
2573 1894 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3354 4312 442
Size: 416 53 83 399 24 1937 958 1753
Fragments arranged by size:
1937 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2184 2559 4283 1239
Size: 945 375 1724 2579
Fragments arranged by size:
2579 1724 945 375
Bpu10I CC'TnA_GC
Cuts at: 2315 2315
Size: 5623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4286 882
Size: 617 2787 2219
Fragments arranged by size:
2787 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 3430 3812 4447
Size: 53 83 186 936 678 1219 382 635
Cuts at: 4447 275
Size: 1451
Fragments arranged by size:
1451 1219 936 678 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4056 4454 4728 4875 5286 4056
Size: 398 274 147 411 4393
Fragments arranged by size:
4393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 2079 2109 1178
Size: 30 165 30 600 30 46 30 4692
Fragments arranged by size:
4692 600 165 46 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2239 2240 2835 1186
Size: 841 212 1 595 3974
Fragments arranged by size:
3974 841 595 212 1
BseYI C'CCAG_C
Cuts at: 2319 4826 5322 2319
Size: 2507 496 2620
Fragments arranged by size:
2620 2507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 1163
Size: 573 629 4421
Fragments arranged by size:
4421 629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2643 2842 3684 3769 4840 2643
Size: 199 842 85 1071 3426
Fragments arranged by size:
3426 1071 842 199 85
BsmI GAATG_Cn'
Cuts at: 3134 3227 3134
Size: 93 5530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2643 2842 2942 3684 3769 4840 1391
Size: 399 853 199 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 853 742 399 199 100 85
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2978 844
Size: 2134 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3510 1394
Size: 429 1687 3507
Fragments arranged by size:
3507 1687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2542 4267 4454 1700
Size: 110 732 1725 187 2869
Fragments arranged by size:
2869 1725 732 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5623
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 2841 3683 4695 2841
Size: 842 1012 3769
Fragments arranged by size:
3769 1012 842
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5623
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 2855 1184
Size: 1671 3952
BstXI CCAn_nnnn'nTGG
Cuts at: 2744 2867 2744
Size: 123 5500
BstYI r'GATC_y
Cuts at: 1972 2338 2395 2813 2953 3320 3705 3722 5174
Size: 366 57 418 140 367 385 17 1452
Cuts at: 5174 5260 5272 5618 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 418 385 367 366 346 140
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2091 2646 513
Size: 552 1026 555 3490
Fragments arranged by size:
3490 1026 555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4884
Fragments arranged by size:
4884 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3933 3953 3139
Size: 794 20 4809
Fragments arranged by size:
4809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2440 2927 2440
Size: 487 5136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 3959 5588
Size: 54 1425 50 275 18 1240 889 1629
Cuts at: 5588 8
Size: 43
Fragments arranged by size:
1629 1425 1240 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2583 2830 2842 3553 1353
Size: 1230 247 12 711 3423
Fragments arranged by size:
3423 1230 711 247 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2166 4210 4584 4730 387
Size: 1064 138 577 2044 374 146 1280
Fragments arranged by size:
2044 1280 1064 577 374 146 138
EcoICRI GAG'CTC
Cuts at: 2641 2840 2641
Size: 199 5424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 2286 2363 2408 2559 2607 3686
Size: 393 552 102 77 45 151 48 1079
Cuts at: 3686 4283 5070 5338 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1079 787 597 552 393 268 151
102 77 48 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2403 2403
Size: 5623
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5623
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 2187 4770 1678
Size: 509 2583 2531
Fragments arranged by size:
2583 2531 509
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2615 2647 4264 4296 4338
Size: 32 135 32 1043 32 1617 32 42
Cuts at: 4338 4370 1373
Size: 32 2626
Fragments arranged by size:
2626 1617 1043 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3213 669
Size: 988 281 1029 246 3079
Fragments arranged by size:
3079 1029 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5623
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 3222 3222
Size: 5623
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2172 4737 4921 1305
Size: 684 183 2565 184 2007
Fragments arranged by size:
2565 2007 684 184 183
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3801
Fragments arranged by size:
3801 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3581 3940 4099 518
Size: 648 2415 359 159 2042
Fragments arranged by size:
2415 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2117 2284 3039 3716 4864 5109 1606
Size: 439 72 167 755 677 1148 245 2120
Fragments arranged by size:
2120 1148 755 677 439 245 167 72
NcoI C'CATG_G
Cuts at: 513 1065 2091 2646 513
Size: 552 1026 555 3490
Fragments arranged by size:
3490 1026 555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5623
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2981 4526 1132
Size: 97 1752 1545 2229
Fragments arranged by size:
2229 1752 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5623
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2003 2035 3692 3724 5205 5237 2003
Size: 32 1657 32 1481 32 2389
Fragments arranged by size:
2389 1657 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2975 830
Size: 2145 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
SacI G_AGCT'C
Cuts at: 2643 2842 2643
Size: 199 5424
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SapI GCTCTTCn'nnn_
Cuts at: 2830 2830
Size: 5623
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 2606 3871 5583 2606
Size: 1265 1712 2646
Fragments arranged by size:
2646 1712 1265
SfcI C'TryA_G
Cuts at: 826 1045 2971 4106 4787 4978 826
Size: 219 1926 1135 681 191 1471
Fragments arranged by size:
1926 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5623
SmaI CCC'GGG
Cuts at: 1872 2950 1872
Size: 1078 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3727 4628 4890 820
Size: 197 926 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2981 1132
Size: 1849 3774
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 2313 1517
Size: 796 4827
StyI C'CwwG_G
Cuts at: 513 1065 2091 2646 3062 513
Size: 552 1026 555 416 3074
Fragments arranged by size:
3074 1026 555 552 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3650 3667 3820 4005 4450 325
Size: 1255 2070 17 153 185 445 1498
Fragments arranged by size:
2070 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2604 3869 5581
Size: 274 80 33 51 1443 627 1265 1712
Cuts at: 5581 96
Size: 138
Fragments arranged by size:
1712 1443 1265 627 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 5583 5615 2079
Size: 32 3472 32 2087
Fragments arranged by size:
3472 2087 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2816 2959 2816
Size: 143 5480
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5623
XmaI C'CCGG_G
Cuts at: 1870 2948 1870
Size: 1078 4545
XmnI GAAnn'nnTTC
Cuts at: 3752 3752
Size: 5623
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3431 276
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI