Restriction map for
pFN21AB8267
(Circular) (Six-base) MAPSORT of: pFN21AB8267.seq Check: 4529 from: 1 to: 5398
pFN21AB8267 5398 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3208 278
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 1937
Size: 804 4594
Acc65I G'GTAC_C
Cuts at: 2719 2719
Size: 5398
AclI AA'CG_TT
Cuts at: 1563 3525 3898 1563
Size: 1962 373 3063
Fragments arranged by size:
3063 1962 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3461 4845 5113 1632
Size: 1829 1384 268 1917
Fragments arranged by size:
1917 1829 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5201
AflIII A'CryG_T
Cuts at: 1203 4297 5274 1203
Size: 3094 977 1327
Fragments arranged by size:
3094 1327 977
AgeI A'CCGG_T
Cuts at: 4229 4229
Size: 5398
AhdI GACnn_n'nnGTC
Cuts at: 4127 4127
Size: 5398
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2045 2048 1184
Size: 861 3 4534
Fragments arranged by size:
4534 861 3
AlwNI CAG_nnn'CTG
Cuts at: 2462 2471 3141 4713 2462
Size: 9 670 1572 3147
Fragments arranged by size:
3147 1572 670 9
ApaI G_GGCC'C
Cuts at: 2315 2576 2315
Size: 261 5137
ApaLI G'TGCA_C
Cuts at: 3455 4611 3455
Size: 1156 4242
ApoI r'AATT_y
Cuts at: 1493 2119 2707 2933 3085 5194 1493
Size: 626 588 226 152 2109 1697
Fragments arranged by size:
2109 1697 626 588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3952 160
Size: 872 2920 1606
Fragments arranged by size:
2920 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 2723 1870
Size: 73 340 440 4545
Fragments arranged by size:
4545 440 340 73
BamHI G'GATC_C
Cuts at: 2616 2728 3095 2616
Size: 112 367 4919
Fragments arranged by size:
4919 367 112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2719 4174 618
Size: 325 217 627 303 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2315 2497 2576 2717 1391
Size: 924 182 79 141 4072
Fragments arranged by size:
4072 924 182 141 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3589 3623 1903
Size: 34 1652 34 3678
Fragments arranged by size:
3678 1652 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3290 4500 3290
Size: 1210 4188
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5398
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4009 136
Size: 107 122 71 631 605 2337 1525
Fragments arranged by size:
2337 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5393 5393
Size: 5398
BlpI GC'TnA_GC
Cuts at: 2815 2815
Size: 5398
Bme1580I G_kGCm'C
Cuts at: 1790 2315 2576 3459 4615 1790
Size: 525 261 883 1156 2573
Fragments arranged by size:
2573 1156 883 525 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 3129 4087
Size: 416 53 83 399 24 1080 632 958
Cuts at: 4087 442
Size: 1753
Fragments arranged by size:
1753 1080 958 632 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 2544 4058 1239
Size: 1080 225 1514 2579
Fragments arranged by size:
2579 1514 1080 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3523 4388 4686 3523
Size: 865 298 4235
Fragments arranged by size:
4235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2242 2584 4061 882
Size: 617 743 342 1477 2219
Fragments arranged by size:
2219 1477 743 617 342
BsaAI yAC'GTr
Cuts at: 493 1204 3189 493
Size: 711 1985 2702
Fragments arranged by size:
2702 1985 711
BsaBI GATnn'nnATC
Cuts at: 5392 5392
Size: 5398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3205 3587 4222 275
Size: 53 83 186 936 1672 382 635 1451
Fragments arranged by size:
1672 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3831 4229 4503 4650 5061 3831
Size: 398 274 147 411 4168
Fragments arranged by size:
4168 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5173
Fragments arranged by size:
5173 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2250 2562 2661 1186
Size: 1064 312 99 3923
Fragments arranged by size:
3923 1064 312 99
BseYI C'CCAG_C
Cuts at: 2347 2351 2387 4601 5097 2347
Size: 4 36 2214 496 2648
Fragments arranged by size:
2648 2214 496 36 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4825
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 2848 3609 4637 1540
Size: 275 186 76 771 761 1028 2301
Fragments arranged by size:
2301 1028 771 761 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 2554 3459 3544 4615 2554
Size: 905 85 1071 3337
Fragments arranged by size:
3337 1071 905 85
BsmI GAATG_Cn'
Cuts at: 2667 2909 3002 2667
Size: 242 93 5063
Fragments arranged by size:
5063 242 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2315 2497 2554 2576 2717 3459 3544
Size: 399 525 182 57 22 141 742 85
Cuts at: 3544 4615 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 525 399 182 141 85
57 22
BspEI T'CCGG_A
Cuts at: 5061 5061
Size: 5398
BspHI T'CATG_A
Cuts at: 3287 5017 3287
Size: 1730 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2753 844
Size: 1909 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3285 1394
Size: 429 1462 3507
Fragments arranged by size:
3507 1462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3893 4067 65
Size: 1183 2645 174 1396
Fragments arranged by size:
2645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4042 4229 1700
Size: 110 2232 187 2869
Fragments arranged by size:
2869 2232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5398
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5398
BssSI C'ACGA_G
Cuts at: 3458 4470 3458
Size: 1012 4386
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2384 2462 2471 794
Size: 1590 78 9 3721
Fragments arranged by size:
3721 1590 78 9
BstBI TT'CG_AA
Cuts at: 4202 4202
Size: 5398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5398
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5398
BstYI r'GATC_y
Cuts at: 1972 2079 2616 2728 3095 3480 3497 4949 5035
Size: 107 537 112 367 385 17 1452 86
Cuts at: 5035 5047 5393 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 537 385 367 346 112 107
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2398 2518 513
Size: 552 1333 120 3393
Fragments arranged by size:
3393 1333 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4659
Fragments arranged by size:
4659 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3708 3728 2914
Size: 794 20 4584
Fragments arranged by size:
4584 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5363
DraI TTT'AAA
Cuts at: 2702 2702
Size: 5398
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4405 809
Size: 507 3089 1802
Fragments arranged by size:
3089 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2515 2845
Size: 54 1425 50 275 18 244 441 330
Cuts at: 2845 3734 5363 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 441 330 275 244 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2074 2845 1537
Size: 275 262 771 4090
Fragments arranged by size:
4090 771 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2437 2623 3328 1353
Size: 831 253 186 705 3423
Fragments arranged by size:
3423 831 705 253 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3985 4359 4505 387
Size: 1064 138 2396 374 146 1280
Fragments arranged by size:
2396 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 2544 3461 4058 4845 5113 1239
Size: 393 687 225 917 597 787 268 1524
Fragments arranged by size:
1524 917 787 687 597 393 268 225
EcoNI CCTnn'n_nnAGG
Cuts at: 2628 2628
Size: 5398
EcoO109I rG'GnC_Cy
Cuts at: 2255 2311 2525 2603 2255
Size: 56 214 78 5050
Fragments arranged by size:
5050 214 78 56
EcoRI G'AATT_C
Cuts at: 2707 3085 2707
Size: 378 5020
FspI TGC'GCA
Cuts at: 3904 3904
Size: 5398
HaeII r_GCGC'y
Cuts at: 1678 4545 1678
Size: 2867 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4039 4071 4113 4145 1373
Size: 32 135 32 2467 32 42 32 2626
Fragments arranged by size:
2626 2467 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2742 2988 669
Size: 988 281 804 246 3079
Fragments arranged by size:
3079 988 804 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5398
HpaI GTT'AAC
Cuts at: 2988 2988
Size: 5398
KpnI G_GTAC'C
Cuts at: 2723 2723
Size: 5398
MfeI C'AATT_G
Cuts at: 2997 2997
Size: 5398
MluI A'CGCG_T
Cuts at: 5274 5274
Size: 5398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2522 4512 4696 1305
Size: 1217 1990 184 2007
Fragments arranged by size:
2007 1990 1217 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3576
Fragments arranged by size:
3576 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3356 3715 3874 518
Size: 648 843 1347 359 159 2042
Fragments arranged by size:
2042 1347 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2351 2462 2468 2814 3491 4639 4884 1606
Size: 745 111 6 346 677 1148 245 2120
Fragments arranged by size:
2120 1148 745 677 346 245 111 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4846
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5398
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5398
NotI GC'GGCC_GC
Cuts at: 2845 2845
Size: 5398
NspI r_CATG'y
Cuts at: 1132 1229 2650 2756 4301 1132
Size: 97 1421 106 1545 2229
Fragments arranged by size:
2229 1545 1421 106 97
PasI CC'CwG_GG
Cuts at: 1348 2315 2678 1348
Size: 967 363 4068
Fragments arranged by size:
4068 967 363
PciI A'CATG_T
Cuts at: 4297 4297
Size: 5398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 2399 2582 1108
Size: 1189 102 183 3924
Fragments arranged by size:
3924 1189 183 102
PfoI T'CCnGG_A
Cuts at: 2656 2656
Size: 5398
PmeI GTTT'AAAC
Cuts at: 2702 2702
Size: 5398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 4980 5012 3467
Size: 32 1481 32 3853
Fragments arranged by size:
3853 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2255 2525 2603 2255
Size: 270 78 5050
Fragments arranged by size:
5050 270 78
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5398
PsiI TTA'TAA
Cuts at: 2968 2968
Size: 5398
PspOMI G'GGCC_C
Cuts at: 2311 2572 2311
Size: 261 5137
PspXI vC'TCGA_Gb
Cuts at: 2283 2283
Size: 5398
PstI C_TGCA'G
Cuts at: 830 2037 2070 2380 2467 2750 830
Size: 1207 33 310 87 283 3478
Fragments arranged by size:
3478 1207 310 283 87 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5398
PvuII CAG'CTG
Cuts at: 2351 2468 2351
Size: 117 5281
SalI G'TCGA_C
Cuts at: 1936 2740 1936
Size: 804 4594
SanDI GG'GwC_CC
Cuts at: 2603 2603
Size: 5398
SbfI CC_TGCA'GG
Cuts at: 2070 2380 2750 2070
Size: 310 370 4718
Fragments arranged by size:
4718 370 310
ScaI AGT'ACT
Cuts at: 3646 5358 3646
Size: 1712 3686
SfcI C'TryA_G
Cuts at: 826 1045 2033 2066 2376 2463 2746 3881 4562
Size: 219 988 33 310 87 283 1135 681
Cuts at: 4562 4753 826
Size: 191 1471
Fragments arranged by size:
1471 1135 988 681 310 283 219 191
87 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5398
SmaI CCC'GGG
Cuts at: 1872 2725 1872
Size: 853 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2283 3502 4403 4665 820
Size: 197 926 340 1219 901 262 1553
Fragments arranged by size:
1553 1219 926 901 340 262 197
SnaBI TAC'GTA
Cuts at: 493 3189 493
Size: 2696 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5398
SphI G_CATG'C
Cuts at: 1132 2650 2756 1132
Size: 1518 106 3774
Fragments arranged by size:
3774 1518 106
SspI AAT'ATT
Cuts at: 5 52 3322 5
Size: 47 3270 2081
Fragments arranged by size:
3270 2081 47
StuI AGG'CCT
Cuts at: 1517 2691 1517
Size: 1174 4224
StyI C'CwwG_G
Cuts at: 513 1065 2837 513
Size: 552 1772 3074
Fragments arranged by size:
3074 1772 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 3425 3442 3595 3780 4225 325
Size: 1255 615 1230 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 615 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3644 5356 96
Size: 274 80 33 51 1443 1667 1712 138
Fragments arranged by size:
1712 1667 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5358 5390 5358
Size: 32 5366
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5398
XbaI T'CTAG_A
Cuts at: 2734 2734
Size: 5398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 2510
Size: 5398
XhoI C'TCGA_G
Cuts at: 1943 2283 1943
Size: 340 5058
XmaI C'CCGG_G
Cuts at: 1870 2723 1870
Size: 853 4545
XmnI GAAnn'nnTTC
Cuts at: 3527 3527
Size: 5398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3206 276
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI