Restriction map for
pFN21AB8265
(Circular) (Six-base) MAPSORT of: pFN21AB8265.seq Check: 329 from: 1 to: 6058
pFN21AB8265 6058 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 2616 3401 1937
Size: 271 408 785 4594
Fragments arranged by size:
4594 785 408 271
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2384 4121 5505 5773 1632
Size: 514 238 1737 1384 268 1917
Fragments arranged by size:
1917 1737 1384 514 268 238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 2393 4889 2393
Size: 2496 3562
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 3089 1166
Size: 1923 4135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2204 1184
Size: 1020 5038
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2076
Size: 32 6026
AlwNI CAG_nnn'CTG
Cuts at: 3801 5373 3801
Size: 1572 4486
ApaLI G'TGCA_C
Cuts at: 2389 4115 5271 2389
Size: 1726 1156 3176
Fragments arranged by size:
3176 1726 1156
ApoI r'AATT_y
Cuts at: 1493 2367 2765 3367 3593 3745 5854 1493
Size: 874 398 602 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 602 398 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 2316 3383 1870
Size: 73 250 123 1067 4545
Fragments arranged by size:
4545 1067 250 123 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3217 3250 3217
Size: 33 6025
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 2524 2782 3042 3091
Size: 325 217 627 459 278 258 260 49
Cuts at: 3091 3379 4834 618
Size: 288 1455 1842
Fragments arranged by size:
1842 1455 627 459 325 288 278 260
258 217 49
BanII G_rGCy'C
Cuts at: 1391 2073 2556 2746 3172 3377 1391
Size: 682 483 190 426 205 4072
Fragments arranged by size:
4072 682 483 426 205 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 928
Size: 971 491 4596
Fragments arranged by size:
4596 971 491
BbvCI CC'TCA_GC
Cuts at: 3159 3159
Size: 6058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4249 4283 1903
Size: 34 50 34 2228 34 3678
Fragments arranged by size:
3678 2228 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3051 4669 136
Size: 107 122 71 631 605 1379 1618 1525
Fragments arranged by size:
1618 1525 1379 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3137 3475 3137
Size: 338 5720
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2325 2393 2787 3047 4119 5275 1790
Size: 403 132 68 394 260 1072 1156 2573
Fragments arranged by size:
2573 1156 1072 403 394 260 132 68
BmgBI CAC'GTC
Cuts at: 1318 2971 1318
Size: 1653 4405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3789 4747 442
Size: 416 53 83 399 24 2372 958 1753
Fragments arranged by size:
2372 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 2609 4718 1239
Size: 1286 84 2109 2579
Fragments arranged by size:
2579 2109 1286 84
Bpu10I CC'TnA_GC
Cuts at: 2271 3159 2271
Size: 888 5170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3308 4183 5048 5346 3308
Size: 875 865 298 4020
Fragments arranged by size:
4020 875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3096 4721 882
Size: 617 1597 1625 2219
Fragments arranged by size:
2219 1625 1597 617
BsaAI yAC'GTr
Cuts at: 493 1204 2188 3229 3849 493
Size: 711 984 1041 620 2702
Fragments arranged by size:
2702 1041 984 711 620
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2393 4491 4889 5163 5310 5721 2393
Size: 2098 398 274 147 411 2730
Fragments arranged by size:
2730 2098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2151 3317 1186
Size: 965 1166 3927
Fragments arranged by size:
3927 1166 965
BseYI C'CCAG_C
Cuts at: 2064 5261 5757 2064
Size: 3197 496 2365
Fragments arranged by size:
3197 2365 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2393 2595 4119 4204 5275 2073
Size: 320 202 1524 85 1071 2856
Fragments arranged by size:
2856 1524 1071 320 202 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
BsmBI CGTCTCn'nnnn_
Cuts at: 2162 2477 2162
Size: 315 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2193 2325 2393 2556 2595 2746
Size: 399 283 120 132 68 163 39 151
Cuts at: 2746 2787 3047 3172 3377 4119 4204 5275 1391
Size: 41 260 125 205 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 260 205 163
151 132 125 120 85 68 41 39
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3413 844
Size: 2569 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 2997 3153 4553 4727 65
Size: 1183 814 935 156 1400 174 1396
Fragments arranged by size:
1400 1396 1183 935 814 174 156
BsrFI r'CCGG_y
Cuts at: 1700 1810 2393 4702 4889 1700
Size: 110 583 2309 187 2869
Fragments arranged by size:
2869 2309 583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 794
Size: 1267 4791
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2102 2426 2908 2102
Size: 324 482 5252
Fragments arranged by size:
5252 482 324
BstYI r'GATC_y
Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053
Size: 1416 367 385 17 1452 86 12 346
Cuts at: 6053 1972
Size: 1977
Fragments arranged by size:
1977 1452 1416 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2929 2962 513
Size: 552 1864 33 3609
Fragments arranged by size:
3609 1864 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 2718 3574 4368 4388 2718
Size: 856 794 20 4388
Fragments arranged by size:
4388 856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2209 2244 3136 3171 529
Size: 35 1645 35 892 35 3416
Fragments arranged by size:
3416 1645 892 35 35 35
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2104 2811
Size: 54 1425 50 275 18 202 72 707
Cuts at: 2811 3108 3505 4394 6023 8
Size: 297 397 889 1629 43
Fragments arranged by size:
1629 1425 889 707 397 297 275 202
72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 3988 1353
Size: 914 1721 3423
Fragments arranged by size:
3423 1721 914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 6058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2384 2525 2609 4121 4718 5505
Size: 393 514 238 141 84 1512 597 787
Cuts at: 5505 5773 1239
Size: 268 1524
Fragments arranged by size:
1524 1512 787 597 514 393 268 238
141 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3110 3290 3110
Size: 180 5878
EcoRI G'AATT_C
Cuts at: 2367 2765 3367 3745 2367
Size: 398 602 378 4680
Fragments arranged by size:
4680 602 398 378
EcoRV GAT'ATC
Cuts at: 2659 2659
Size: 6058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2178 2668 5205 1678
Size: 500 490 2537 2531
Fragments arranged by size:
2537 2531 500 490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373
Size: 32 135 32 3127 32 42 32 2626
Fragments arranged by size:
3127 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2755 3402 3648 669
Size: 988 281 817 647 246 3079
Fragments arranged by size:
3079 988 817 647 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 5172 5356 1305
Size: 1035 2832 184 2007
Fragments arranged by size:
2832 2007 1035 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2034 2106 2813 3110 10
Size: 54 1425 343 202 72 707 297 2958
Fragments arranged by size:
2958 1425 707 343 297 202 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 4016 4375 4534 518
Size: 648 1923 927 359 159 2042
Fragments arranged by size:
2042 1923 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2543 3474 4151 5299 5544 1606
Size: 937 931 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 931 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2929 513
Size: 552 1864 3642
Fragments arranged by size:
3642 1864 552
NdeI CA'TA_TG
Cuts at: 387 2818 387
Size: 2431 3627
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3136 3416 4961 1132
Size: 97 1907 280 1545 2229
Fragments arranged by size:
2229 1907 1545 280 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3034 1108
Size: 1926 4132
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PmlI CAC'GTG
Cuts at: 2188 2188
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2640 1613
Size: 1027 5031
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PstI C_TGCA'G
Cuts at: 830 3410 830
Size: 2580 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2543 2543
Size: 6058
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 6058
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SexAI A'CCwGG_T
Cuts at: 2778 2778
Size: 6058
SfcI C'TryA_G
Cuts at: 826 1045 2544 2865 3406 4541 5222 5413 826
Size: 219 1499 321 541 1135 681 191 1471
Fragments arranged by size:
1499 1471 1135 681 541 321 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2195 3385 1872
Size: 323 1190 4545
Fragments arranged by size:
4545 1190 323
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3287 4162 5063 5325 820
Size: 197 926 1344 875 901 262 1553
Fragments arranged by size:
1553 1344 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3136 3416 1132
Size: 2004 280 3774
Fragments arranged by size:
3774 2004 280
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 3164 1517
Size: 1647 4411
StyI C'CwwG_G
Cuts at: 513 1065 2929 3111 3497 513
Size: 552 1864 182 386 3074
Fragments arranged by size:
3074 1864 552 386 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2379 2591 3221 4085 4102 4255 4440
Size: 1255 799 212 630 864 17 153 185
Cuts at: 4440 4885 325
Size: 445 1498
Fragments arranged by size:
1498 1255 864 799 630 445 212 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4304 6016 96
Size: 274 80 33 51 1443 2327 1712 138
Fragments arranged by size:
2327 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6018 6050 6018
Size: 32 6026
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2426
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 2193 3383 1870
Size: 323 1190 4545
Fragments arranged by size:
4545 1190 323
XmnI GAAnn'nnTTC
Cuts at: 2440 4187 2440
Size: 1747 4311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI