Restriction map for
pFN21AB8263
(Circular) (Six-base) MAPSORT of: pFN21AB8263.seq Check: 3757 from: 1 to: 5668
pFN21AB8263 5668 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 2967 3795 4168 1563
Size: 1404 828 373 3063
Fragments arranged by size:
3063 1404 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3731 5115 5383 1632
Size: 2099 1384 268 1917
Fragments arranged by size:
2099 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 3411 4983 3411
Size: 1572 4096
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 2512 2993 1870
Size: 73 558 11 481 4545
Fragments arranged by size:
4545 558 481 73 11
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2516 2989 4444 618
Size: 325 217 627 729 473 1455 1842
Fragments arranged by size:
1842 1455 729 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2987 1391
Size: 1596 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2284 928
Size: 971 385 4312
Fragments arranged by size:
4312 971 385
BbvCI CC'TCA_GC
Cuts at: 2824 2824
Size: 5668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2665 2699 3859 3893 1903
Size: 34 728 34 1160 34 3678
Fragments arranged by size:
3678 1160 728 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 2088 2278
Size: 107 122 71 631 605 380 36 190
Cuts at: 2278 4279 136
Size: 2001 1525
Fragments arranged by size:
2001 1525 631 605 380 190 122 107
71 36
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2519 3729 4885 1790
Size: 729 1210 1156 2573
Fragments arranged by size:
2573 1210 1156 729
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2438 1056
Size: 1382 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
Bpu10I CC'TnA_GC
Cuts at: 2487 2824 2487
Size: 337 5331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 3793 4658 4956 2921
Size: 872 865 298 3633
Fragments arranged by size:
3633 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2530 2910 4331 882
Size: 617 1031 380 1421 2219
Fragments arranged by size:
2219 1421 1031 617 380
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 2471 5662 2471
Size: 3191 2477
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2551 4101 4499 4773 4920 5331 2551
Size: 1550 398 274 147 411 2888
Fragments arranged by size:
2888 1550 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2154 2157 2184 2187 1186
Size: 968 3 27 3 4667
Fragments arranged by size:
4667 968 27 3 3
BseYI C'CCAG_C
Cuts at: 2718 4871 5367 2718
Size: 2153 496 3019
Fragments arranged by size:
3019 2153 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 3118 3879 4907 1540
Size: 275 186 46 1071 761 1028 2301
Fragments arranged by size:
2301 1071 1028 761 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 3729 3814 4885 3729
Size: 85 1071 4512
Fragments arranged by size:
4512 1071 85
BsmI GAATG_Cn'
Cuts at: 2755 3179 3272 2755
Size: 424 93 5151
Fragments arranged by size:
5151 424 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2519 2987 3729 3814 4885 1391
Size: 399 729 468 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 468 399 85
BspEI T'CCGG_A
Cuts at: 2551 5331 2551
Size: 2780 2888
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 844
Size: 2179 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2427 2589 3555 1394
Size: 429 604 162 966 3507
Fragments arranged by size:
3507 966 604 429 162
BsrDI GCAATG_nn'
Cuts at: 65 1248 2694 4163 4337 65
Size: 1183 1446 1469 174 1396
Fragments arranged by size:
1469 1446 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2940 96
Size: 2844 2824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 2060 2323 1184
Size: 876 263 4529
Fragments arranged by size:
4529 876 263
BstXI CCAn_nnnn'nTGG
Cuts at: 2204 2335 2591 2204
Size: 131 256 5281
Fragments arranged by size:
5281 256 131
BstYI r'GATC_y
Cuts at: 1972 2466 2998 3365 3750 3767 5219 5305 5317
Size: 494 532 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 532 494 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2026 2065 2520 2925 513
Size: 552 961 39 455 405 3256
Fragments arranged by size:
3256 961 552 455 405 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2668 487
Size: 683 56 1442 3487
Fragments arranged by size:
3487 1442 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3978 3998 3184
Size: 794 20 4854
Fragments arranged by size:
4854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DraIII CAC_nnn'GTG
Cuts at: 2785 2785
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2593 2613
Size: 54 1425 50 275 18 214 549 20
Cuts at: 2613 3115 4004 5633 8
Size: 502 889 1629 43
Fragments arranged by size:
1629 1425 889 549 502 275 214 54
50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2044 3115 1537
Size: 275 232 1071 4090
Fragments arranged by size:
4090 1071 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2173 2360 2615 3598 1353
Size: 820 187 255 983 3423
Fragments arranged by size:
3423 983 820 255 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2130 4255 4629 4775 387
Size: 1064 138 541 2125 374 146 1280
Fragments arranged by size:
2125 1280 1064 541 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3731 4328 5115 5383 1239
Size: 393 2099 597 787 268 1524
Fragments arranged by size:
2099 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3012 3258 669
Size: 988 281 957 117 246 3079
Fragments arranged by size:
3079 988 957 281 246 117
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 10
Size: 54 1425 343 783 3063
Fragments arranged by size:
3063 1425 783 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2202 2280 2286 2639 2687 3626 3985
Size: 648 1036 78 6 353 48 939 359
Cuts at: 3985 4144 518
Size: 159 2042
Fragments arranged by size:
2042 1036 939 648 359 353 159 78
48 6
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5116
NdeI CA'TA_TG
Cuts at: 387 2908 387
Size: 2521 3147
NheI G'CTAG_C
Cuts at: 1052 2434 1052
Size: 1382 4286
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2680 3026 4571 1132
Size: 97 1451 346 1545 2229
Fragments arranged by size:
2229 1545 1451 346 97
PasI CC'CwG_GG
Cuts at: 1348 2342 1348
Size: 994 4674
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2926 1108
Size: 1818 3850
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SexAI A'CCwGG_T
Cuts at: 2334 2334
Size: 5668
SfcI C'TryA_G
Cuts at: 826 1045 3016 4151 4832 5023 826
Size: 219 1971 1135 681 191 1471
Fragments arranged by size:
1971 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2900 3772 4673 4935 820
Size: 197 926 957 872 901 262 1553
Fragments arranged by size:
1553 957 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 2680 3026 1132
Size: 1548 346 3774
Fragments arranged by size:
3774 1548 346
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2955 3107 513
Size: 552 1890 152 3074
Fragments arranged by size:
3074 1890 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2441 2676 3695 3712 3865 4050 4495
Size: 1255 861 235 1019 17 153 185 445
Cuts at: 4495 325
Size: 1498
Fragments arranged by size:
1498 1255 1019 861 445 235 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2940 3914 5626
Size: 274 80 33 51 1443 963 974 1712
Cuts at: 5626 96
Size: 138
Fragments arranged by size:
1712 1443 974 963 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 5628 5660 2769
Size: 32 2827 32 2777
Fragments arranged by size:
2827 2777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2420 3797 2420
Size: 1377 4291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI