Restriction map for
pFN21AB8259
(Circular) (Six-base) MAPSORT of: pFN21AB8259.seq Check: 3947 from: 1 to: 5602
pFN21AB8259 5602 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2139 2792 2139
Size: 653 4949
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 2945 1937
Size: 429 579 4594
Fragments arranged by size:
4594 579 429
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2612 2717 3665 5049 5317 1632
Size: 494 486 105 948 1384 268 1917
Fragments arranged by size:
1917 1384 948 494 486 268 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2822 1166
Size: 1656 3946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2306 1184
Size: 1122 4480
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2302 2927 1870
Size: 73 202 157 625 4545
Fragments arranged by size:
4545 625 202 157 73
BamHI G'GATC_C
Cuts at: 2326 2932 3299 2326
Size: 606 367 4629
Fragments arranged by size:
4629 606 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2359 2395 2923 4378 618
Size: 325 217 627 572 36 528 1455 1842
Fragments arranged by size:
1842 1455 627 572 528 325 217 36
BanII G_rGCy'C
Cuts at: 1391 2186 2850 2921 1391
Size: 795 664 71 4072
Fragments arranged by size:
4072 795 664 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BfrBI ATG'CAT
Cuts at: 2523 2523
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 4213 136
Size: 107 122 71 631 605 646 1895 1525
Fragments arranged by size:
1895 1525 646 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2362 3663 4819 1790
Size: 572 1301 1156 2573
Fragments arranged by size:
2573 1301 1156 572
BmgBI CAC'GTC
Cuts at: 1318 2298 1318
Size: 980 4622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 4262 1239
Size: 881 2142 2579
Fragments arranged by size:
2579 2142 881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 3393 493
Size: 711 1269 920 2702
Fragments arranged by size:
2702 1269 920 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2392 4035 4433 4707 4854 5265 2392
Size: 1643 398 274 147 411 2729
Fragments arranged by size:
2729 1643 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2374 2404 1178
Size: 30 165 30 971 30 4376
Fragments arranged by size:
4376 971 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2207 2690 1186
Size: 850 171 483 4098
Fragments arranged by size:
4098 850 483 171
BseYI C'CCAG_C
Cuts at: 2330 2649 4805 5301 2330
Size: 319 2156 496 2631
Fragments arranged by size:
2631 2156 496 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 2794 2815 2846 1163
Size: 573 375 683 21 31 3919
Fragments arranged by size:
3919 683 573 375 31 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2742 3663 3748 4819 2186
Size: 556 921 85 1071 2969
Fragments arranged by size:
2969 1071 921 556 85
BsmI GAATG_Cn'
Cuts at: 2525 3113 3206 2525
Size: 588 93 4921
Fragments arranged by size:
4921 588 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2362 2742 2850 2921 3663 3748
Size: 399 396 176 380 108 71 742 85
Cuts at: 3748 4819 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 380 176 108
85 71
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2622 2792 2957 844
Size: 1295 483 170 165 3489
Fragments arranged by size:
3489 1295 483 170 165
BsrBI CCG'CTC
Cuts at: 1394 1823 2272 3489 1394
Size: 429 449 1217 3507
Fragments arranged by size:
3507 1217 449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2199 4097 4271 65
Size: 1183 951 1898 174 1396
Fragments arranged by size:
1898 1396 1183 951 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2427 794
Size: 1633 3969
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 2059 1184
Size: 875 4727
BstYI r'GATC_y
Cuts at: 1972 2326 2703 2932 3299 3684 3701 5153 5239
Size: 354 377 229 367 385 17 1452 86
Cuts at: 5239 5251 5597 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 377 367 354 346 229
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 4030
Fragments arranged by size:
4030 1020 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2845 2860 2880 2895 529
Size: 35 2281 15 20 15 3236
Fragments arranged by size:
3236 2281 35 20 15 15
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2475 2682
Size: 54 1425 50 275 18 378 267 207
Cuts at: 2682 3049 3938 5567 8
Size: 367 889 1629 43
Fragments arranged by size:
1629 1425 889 378 367 275 267 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2063 3532 1353
Size: 660 50 1469 3423
Fragments arranged by size:
3423 1469 660 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2635 4189 4563 4709 387
Size: 1064 138 1046 1554 374 146 1280
Fragments arranged by size:
1554 1280 1064 1046 374 146 138
EcoICRI GAG'CTC
Cuts at: 2184 2184
Size: 5602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2126 2612 2717 3665 4262 5049
Size: 393 488 6 486 105 948 597 787
Cuts at: 5049 5317 1239
Size: 268 1524
Fragments arranged by size:
1524 948 787 597 488 486 393 268
105 6
EcoO109I rG'GnC_Cy
Cuts at: 2320 2320
Size: 5602
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 2420 4108 2420
Size: 1688 3914
HaeII r_GCGC'y
Cuts at: 1678 2500 4749 1678
Size: 822 2249 2531
Fragments arranged by size:
2531 2249 822
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2486 2518 4243 4275 4317
Size: 32 135 32 914 32 1725 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 1725 914 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2367 2946 3192 669
Size: 988 281 226 203 579 246 3079
Fragments arranged by size:
3079 988 579 281 246 226 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2518 2610 4716 4900 1305
Size: 1213 92 2106 184 2007
Fragments arranged by size:
2106 2007 1213 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2477 2684 10
Size: 54 1425 343 378 267 207 2928
Fragments arranged by size:
2928 1425 378 343 267 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2822 3560 3919 4078 518
Size: 648 1383 273 738 359 159 2042
Fragments arranged by size:
2042 1383 738 648 359 273 159
MspA1I CmG'CkG
Cuts at: 1606 2447 2889 3018 3695 4843 5088 1606
Size: 841 442 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 841 677 442 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 4030
Fragments arranged by size:
4030 1020 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NsiI A_TGCA'T
Cuts at: 2525 2525
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2287 2960 4505 1132
Size: 97 1058 673 1545 2229
Fragments arranged by size:
2229 1545 1058 673 97
PasI CC'CwG_GG
Cuts at: 1348 2355 1348
Size: 1007 4595
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PfoI T'CCnGG_A
Cuts at: 2228 2241 2529 2228
Size: 13 288 5301
Fragments arranged by size:
5301 288 13
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PmlI CAC'GTG
Cuts at: 2473 2473
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspXI vC'TCGA_Gb
Cuts at: 2302 2302
Size: 5602
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 5570
PstI C_TGCA'G
Cuts at: 830 2457 2954 830
Size: 1627 497 3478
Fragments arranged by size:
3478 1627 497
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SacI G_AGCT'C
Cuts at: 2186 2186
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2365 2944 1936
Size: 429 579 4594
Fragments arranged by size:
4594 579 429
SapI GCTCTTCn'nnn_
Cuts at: 2063 2063
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2453 2950 4085 4766 4957 826
Size: 219 1408 497 1135 681 191 1471
Fragments arranged by size:
1471 1408 1135 681 497 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 3706 4607 4869 820
Size: 197 926 359 1404 901 262 1553
Fragments arranged by size:
1553 1404 926 901 359 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2287 2960 1132
Size: 1155 673 3774
Fragments arranged by size:
3774 1155 673
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2085 2721 3041 513
Size: 552 1020 636 320 3074
Fragments arranged by size:
3074 1020 636 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2371 2374 2403 2406 5562 5594 2371
Size: 3 29 3 3156 32 2379
Fragments arranged by size:
3156 2379 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 2302 1943
Size: 359 5243
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PpuMI PspOMI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI