Restriction map for
pFN21AB8256
(Circular) (Six-base) MAPSORT of: pFN21AB8256.seq Check: 3347 from: 1 to: 5770
pFN21AB8256 5770 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2514 2514
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2052 2837 3113 1937
Size: 115 785 276 4594
Fragments arranged by size:
4594 785 276 115
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 3833 5217 5485 1632
Size: 995 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 995 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 3000 4601 3000
Size: 1601 4169
AhdI GACnn_n'nnGTC
Cuts at: 2167 4499 2167
Size: 2332 3438
AleI CACnn'nnGTG
Cuts at: 1166 2528 1166
Size: 1362 4408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2231 1184
Size: 1047 4723
AlwNI CAG_nnn'CTG
Cuts at: 2080 2770 3513 5085 2080
Size: 690 743 1572 2765
Fragments arranged by size:
2765 1572 743 690
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2282 3079 3305 3457 5566 1493
Size: 789 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 789 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2640 3095 1870
Size: 73 697 455 4545
Fragments arranged by size:
4545 697 455 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2502 2644 3091 4546 618
Size: 325 217 627 715 142 447 1455 1842
Fragments arranged by size:
1842 1455 715 627 447 325 217 142
BanII G_rGCy'C
Cuts at: 1391 2858 2938 3089 1391
Size: 1467 80 151 4072
Fragments arranged by size:
4072 1467 151 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3031 3052 928
Size: 971 1132 21 3646
Fragments arranged by size:
3646 1132 971 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2098 2132 3961 3995 1903
Size: 34 161 34 1829 34 3678
Fragments arranged by size:
3678 1829 161 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2496 4381 136
Size: 107 122 71 631 605 824 1885 1525
Fragments arranged by size:
1885 1525 824 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 2457 3187 2457
Size: 730 5040
Bme1580I G_kGCm'C
Cuts at: 1790 2647 3831 4987 1790
Size: 857 1184 1156 2573
Fragments arranged by size:
2573 1184 1156 857
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 3501 4459
Size: 416 53 83 399 24 712 1372 958
Cuts at: 4459 442
Size: 1753
Fragments arranged by size:
1753 1372 958 712 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2872 2653
Size: 219 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2447 4430 1239
Size: 1121 87 1983 2579
Fragments arranged by size:
2579 1983 1121 87
Bpu10I CC'TnA_GC
Cuts at: 2318 2318
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3895 4760 5058 2957
Size: 938 865 298 3669
Fragments arranged by size:
3669 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 2539 4433 882
Size: 617 567 473 1894 2219
Fragments arranged by size:
2219 1894 617 567 473
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3000 4203 4601 4875 5022 5433 3000
Size: 1203 398 274 147 411 3337
Fragments arranged by size:
3337 1203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 1178
Size: 30 165 30 780 30 4735
Fragments arranged by size:
4735 780 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2632 1186
Size: 1446 4324
BseYI C'CCAG_C
Cuts at: 2937 4973 5469 2937
Size: 2036 496 3238
Fragments arranged by size:
3238 2036 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3831 3916 4987 3831
Size: 85 1071 4614
Fragments arranged by size:
4614 1071 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2647 2858 2938 3089 3831 3916 4987
Size: 399 857 211 80 151 742 85 1071
Cuts at: 4987 1391
Size: 2174
Fragments arranged by size:
2174 1071 857 742 399 211 151 85
80
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 3125 844
Size: 1670 611 3489
Fragments arranged by size:
3489 1670 611
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2499 3000 4414 4601 1700
Size: 110 689 501 1414 187 2869
Fragments arranged by size:
2869 1414 689 501 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2967 3100 3467 3852 3869 5321 5407 5419
Size: 995 133 367 385 17 1452 86 12
Cuts at: 5419 5765 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 995 385 367 346 133 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2012 513
Size: 552 947 4271
Fragments arranged by size:
4271 947 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2101 487
Size: 683 56 875 4156
Fragments arranged by size:
4156 875 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 2397 3286 4080 4100 2284
Size: 113 889 794 20 3954
Fragments arranged by size:
3954 889 794 113 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2497 3217
Size: 54 1425 50 275 18 465 202 720
Cuts at: 3217 4106 5735 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 720 465 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 3700 1353
Size: 1044 1303 3423
Fragments arranged by size:
3423 1303 1044
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2447 2627 3833 4430 5217 5485
Size: 393 728 87 180 1206 597 787 268
Cuts at: 5485 1239
Size: 1524
Fragments arranged by size:
1524 1206 787 728 597 393 268 180
87
EcoO109I rG'GnC_Cy
Cuts at: 2077 2077
Size: 5770
EcoRI G'AATT_C
Cuts at: 2282 3079 3457 2282
Size: 797 378 4595
Fragments arranged by size:
4595 797 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2183 2185 2215 2561
Size: 32 135 32 581 30 2 30 346
Cuts at: 2561 2593 2737 2769 4411 4443 4485 4517 1373
Size: 32 144 32 1642 32 42 32 2626
Fragments arranged by size:
2626 1642 581 346 144 135 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3114 3360 669
Size: 988 281 900 276 246 3079
Fragments arranged by size:
3079 988 900 281 276 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 2841 3369 2841
Size: 528 5242
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2293 2544 3021 4884 5068 1305
Size: 988 251 477 1863 184 2007
Fragments arranged by size:
2007 1863 988 477 251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3483
Fragments arranged by size:
3483 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2528 2741 3053 3728 4087 4246
Size: 648 1125 237 213 312 675 359 159
Cuts at: 4246 518
Size: 2042
Fragments arranged by size:
2042 1125 675 648 359 312 237 213
159
MspA1I CmG'CkG
Cuts at: 1606 2014 2770 2941 3186 3863 5011 5256 1606
Size: 408 756 171 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 756 677 408 245 245 171
NaeI GCC'GGC
Cuts at: 2501 2501
Size: 5770
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5218
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2499 2499
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 3128 4673 1132
Size: 97 1899 1545 2229
Fragments arranged by size:
2229 1899 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3003 1108
Size: 1895 3875
PfoI T'CCnGG_A
Cuts at: 2463 2463
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2077
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 2075 2144 1613
Size: 462 69 5239
Fragments arranged by size:
5239 462 69
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2220 2511 2775 3122 830
Size: 1390 291 264 347 3478
Fragments arranged by size:
3478 1390 347 291 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2770 2941 2770
Size: 171 5599
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 2836 3112 1936
Size: 900 276 4594
Fragments arranged by size:
4594 900 276
SapI GCTCTTCn'nnn_
Cuts at: 2397 2397
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2978 4018 5730 2978
Size: 1040 1712 3018
Fragments arranged by size:
3018 1712 1040
SfcI C'TryA_G
Cuts at: 826 1045 2216 2507 2771 3118 4253 4934 5125
Size: 219 1171 291 264 347 1135 681 191
Cuts at: 5125 826
Size: 1471
Fragments arranged by size:
1471 1171 1135 681 347 291 264 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 2642 3097 1872
Size: 770 455 4545
Fragments arranged by size:
4545 770 455
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2972 3874 4775 5037 820
Size: 197 926 1029 902 901 262 1553
Fragments arranged by size:
1553 1029 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2481 2724 3209 513
Size: 552 1416 243 485 3074
Fragments arranged by size:
3074 1416 552 485 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2976 4016 5728
Size: 274 80 33 51 1443 999 1040 1712
Cuts at: 5728 96
Size: 138
Fragments arranged by size:
1712 1443 1040 999 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2684 2743 2527
Size: 157 59 5554
Fragments arranged by size:
5554 157 59
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 2640 3095 1870
Size: 770 455 4545
Fragments arranged by size:
4545 770 455
XmnI GAAnn'nnTTC
Cuts at: 2904 3899 2904
Size: 995 4775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI