Restriction map for
pFN21AB8255
(Circular) (Six-base) MAPSORT of: pFN21AB8255.seq Check: 6736 from: 1 to: 5656
pFN21AB8255 5656 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2926 2926
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 2710 2834 3719 5103 5371 1632
Size: 944 134 124 885 1384 268 1917
Fragments arranged by size:
1917 1384 944 885 268 134 124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2953 4555 5532 1203
Size: 1750 1602 977 1327
Fragments arranged by size:
1750 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 2857 1166
Size: 1691 3965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2896 3399 4971 2896
Size: 503 1572 3581
Fragments arranged by size:
3581 1572 503
ApaLI G'TGCA_C
Cuts at: 2860 3713 4869 2860
Size: 853 1156 3647
Fragments arranged by size:
3647 1156 853
ApoI r'AATT_y
Cuts at: 1493 2163 2965 3191 3343 5452 1493
Size: 670 802 226 152 2109 1697
Fragments arranged by size:
2109 1697 802 670 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2376 2981 1870
Size: 73 166 267 605 4545
Fragments arranged by size:
4545 605 267 166 73
BamHI G'GATC_C
Cuts at: 2615 2986 3353 2615
Size: 371 367 4918
Fragments arranged by size:
4918 371 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2288 2977 4432 618
Size: 325 217 627 243 258 689 1455 1842
Fragments arranged by size:
1842 1455 689 627 325 258 243 217
BanII G_rGCy'C
Cuts at: 1391 2975 1391
Size: 1584 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 928
Size: 971 665 4020
Fragments arranged by size:
4020 971 665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2580 4267
Size: 107 122 71 631 605 377 531 1687
Cuts at: 4267 136
Size: 1525
Fragments arranged by size:
1687 1525 631 605 531 377 122 107
71
BglII A'GATC_T
Cuts at: 2775 5651 2775
Size: 2876 2780
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2291 2864 3717 4873 1790
Size: 501 573 853 1156 2573
Fragments arranged by size:
2573 1156 853 573 501
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2851 3387 4345
Size: 416 53 83 399 24 1434 536 958
Cuts at: 4345 442
Size: 1753
Fragments arranged by size:
1753 1434 958 536 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2592 2652 4316 1239
Size: 1353 60 1664 2579
Fragments arranged by size:
2579 1664 1353 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 3781 4646 4944 2021
Size: 1760 865 298 2733
Fragments arranged by size:
2733 1760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 4319 882
Size: 617 645 2175 2219
Fragments arranged by size:
2219 2175 645 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2590 2620 1178
Size: 30 165 30 1187 30 4214
Fragments arranged by size:
4214 1187 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2209 2335 1186
Size: 1023 126 4507
Fragments arranged by size:
4507 1023 126
BseYI C'CCAG_C
Cuts at: 2052 2511 2765 4859 5355 2052
Size: 459 254 2094 496 2353
Fragments arranged by size:
2353 2094 496 459 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2179 2864 3717 3802 4873 2179
Size: 685 853 85 1071 2962
Fragments arranged by size:
2962 1071 853 685 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2506 2506
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2291 2864 2975 3717 3802 4873
Size: 399 389 112 573 111 742 85 1071
Cuts at: 4873 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 573 399 389 112 111
85
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 3011 844
Size: 2082 85 3489
Fragments arranged by size:
3489 2082 85
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2355 4300 4487 1700
Size: 110 545 1945 187 2869
Fragments arranged by size:
2869 1945 545 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2296 3716 4728 2296
Size: 1420 1012 3224
Fragments arranged by size:
3224 1420 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 2056 2938 1184
Size: 872 882 3902
Fragments arranged by size:
3902 882 872
BstYI r'GATC_y
Cuts at: 1972 2275 2372 2615 2662 2775 2986 3353 3738
Size: 303 97 243 47 113 211 367 385
Cuts at: 3738 3755 5207 5293 5305 5651 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 303 243 211
113 97 86 47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3966 3986 3172
Size: 794 20 4842
Fragments arranged by size:
4842 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2174 2174
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 4663 809
Size: 507 1206 2141 1802
Fragments arranged by size:
2141 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2227 2572 2644
Size: 54 1425 50 275 18 397 345 72
Cuts at: 2644 2887 3103 3992 5621 8
Size: 243 216 889 1629 43
Fragments arranged by size:
1629 1425 889 397 345 275 243 216
72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3586 1353
Size: 2233 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2092 4243 4617 4763 387
Size: 1064 138 503 2151 374 146 1280
Fragments arranged by size:
2151 1280 1064 503 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2592 2652 2710 2834 3719 4316
Size: 393 944 16 60 58 124 885 597
Cuts at: 4316 5103 5371 1239
Size: 787 268 1524
Fragments arranged by size:
1524 944 885 787 597 393 268 124
60 58 16
EcoO109I rG'GnC_Cy
Cuts at: 2762 2762
Size: 5656
EcoRI G'AATT_C
Cuts at: 2163 2965 3343 2163
Size: 802 378 4476
Fragments arranged by size:
4476 802 378
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2590 2622 4297
Size: 32 135 32 869 32 117 32 1675
Cuts at: 4297 4329 4371 4403 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1675 869 135 117 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 2953 5532 2953
Size: 2579 3077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2229 2574 2646 2889 10
Size: 54 1425 343 397 345 72 243 2777
Fragments arranged by size:
2777 1425 397 345 343 243 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2857 3614 3973 4132 518
Size: 648 960 731 757 359 159 2042
Fragments arranged by size:
2042 960 757 731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2407 1348
Size: 1059 4597
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2658 1108
Size: 1550 4106
PfoI T'CCnGG_A
Cuts at: 2085 2430 2085
Size: 345 5311
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SexAI A'CCwGG_T
Cuts at: 2919 2919
Size: 5656
SfcI C'TryA_G
Cuts at: 826 1045 3004 4139 4820 5011 826
Size: 219 1959 1135 681 191 1471
Fragments arranged by size:
1959 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2109 2376 3760 4661 4923
Size: 197 926 93 73 267 1384 901 262
Cuts at: 4923 820
Size: 1553
Fragments arranged by size:
1553 1384 926 901 267 262 197 93
73
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2319 3095 513
Size: 552 1254 776 3074
Fragments arranged by size:
3074 1254 776 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2399 2443 3683 3700 3853 4038 4483
Size: 1255 819 44 1240 17 153 185 445
Cuts at: 4483 325
Size: 1498
Fragments arranged by size:
1498 1255 1240 819 445 185 153 44
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 2109 2376 1943
Size: 166 267 5223
Fragments arranged by size:
5223 267 166
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI