Restriction map for
pFN21AB8254
(Circular) (Six-base) MAPSORT of: pFN21AB8254.seq Check: 3491 from: 1 to: 5281
pFN21AB8254 5281 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3091 278
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 1937
Size: 687 4594
Acc65I G'GTAC_C
Cuts at: 2602 2602
Size: 5281
AclI AA'CG_TT
Cuts at: 1563 3408 3781 1563
Size: 1845 373 3063
Fragments arranged by size:
3063 1845 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 3344 4728 4996 1632
Size: 779 933 1384 268 1917
Fragments arranged by size:
1917 1384 933 779 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5084
AflIII A'CryG_T
Cuts at: 1203 4180 5157 1203
Size: 2977 977 1327
Fragments arranged by size:
2977 1327 977
AgeI A'CCGG_T
Cuts at: 2152 4112 2152
Size: 1960 3321
AhdI GACnn_n'nnGTC
Cuts at: 4010 4010
Size: 5281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2551 1184
Size: 1367 3914
AlwNI CAG_nnn'CTG
Cuts at: 3024 4596 3024
Size: 1572 3709
ApaLI G'TGCA_C
Cuts at: 3338 4494 3338
Size: 1156 4125
ApoI r'AATT_y
Cuts at: 1493 2590 2816 2968 5077 1493
Size: 1097 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3835 160
Size: 872 2803 1606
Fragments arranged by size:
2803 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 1870
Size: 73 663 4545
Fragments arranged by size:
4545 663 73
BamHI G'GATC_C
Cuts at: 2611 2978 2611
Size: 367 4914
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2310 2602 4057 618
Size: 325 217 627 523 292 1455 1842
Fragments arranged by size:
1842 1455 627 523 325 292 217
BanII G_rGCy'C
Cuts at: 1391 2600 1391
Size: 1209 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3472 3506 1903
Size: 34 50 34 1451 34 3678
Fragments arranged by size:
3678 1451 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2167 2307 3173 4383 2167
Size: 140 866 1210 3065
Fragments arranged by size:
3065 1210 866 140
BclI T'GATC_A
Cuts at: 1548 2064 1548
Size: 516 4765
BfrBI ATG'CAT
Cuts at: 2084 2084
Size: 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3892 136
Size: 107 122 71 631 605 337 1883 1525
Fragments arranged by size:
1883 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5276 5276
Size: 5281
BlpI GC'TnA_GC
Cuts at: 2698 2698
Size: 5281
Bme1580I G_kGCm'C
Cuts at: 1790 3342 4498 1790
Size: 1552 1156 2573
Fragments arranged by size:
2573 1552 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3970 442
Size: 416 53 83 399 24 1595 958 1753
Fragments arranged by size:
1753 1595 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5281
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2549 2549
Size: 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 2489 3941 1239
Size: 873 377 1452 2579
Fragments arranged by size:
2579 1452 873 377
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 5281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2483 3406 4271 4569 2483
Size: 923 865 298 3195
Fragments arranged by size:
3195 923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3944 882
Size: 617 2445 2219
Fragments arranged by size:
2445 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3072 493
Size: 711 1868 2702
Fragments arranged by size:
2702 1868 711
BsaBI GATnn'nnATC
Cuts at: 5275 5275
Size: 5281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3088 3470 4105 275
Size: 53 83 186 936 1555 382 635 1451
Fragments arranged by size:
1555 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2152 3714 4112 4386 4533 4944 2152
Size: 1562 398 274 147 411 2489
Fragments arranged by size:
2489 1562 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5056
Fragments arranged by size:
5056 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5281
BseYI C'CCAG_C
Cuts at: 4484 4980 4484
Size: 496 4785
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4708
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2382 2731 3492 4520 1540
Size: 275 186 381 349 761 1028 2301
Fragments arranged by size:
2301 1028 761 381 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2477 3342 3427 4498 2477
Size: 865 85 1071 3260
Fragments arranged by size:
3260 1071 865 85
BsmI GAATG_Cn'
Cuts at: 2792 2885 2792
Size: 93 5188
BsmBI CGTCTCn'nnnn_
Cuts at: 2028 2028
Size: 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2477 2600 3342 3427 4498 1391
Size: 399 687 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 399 123 85
BspEI T'CCGG_A
Cuts at: 4944 4944
Size: 5281
BspHI T'CATG_A
Cuts at: 3170 4900 3170
Size: 1730 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 844
Size: 1792 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3168 1394
Size: 429 1345 3507
Fragments arranged by size:
3507 1345 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3776 3950 65
Size: 1183 2528 174 1396
Fragments arranged by size:
2528 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2152 3925 4112 1700
Size: 110 342 1773 187 2869
Fragments arranged by size:
2869 1773 342 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5281
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5281
BssSI C'ACGA_G
Cuts at: 3341 4353 3341
Size: 1012 4269
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5281
BstBI TT'CG_AA
Cuts at: 4085 4085
Size: 5281
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5281
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2137
Size: 5281
BstYI r'GATC_y
Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276
Size: 639 367 385 17 1452 86 12 346
Cuts at: 5276 1972
Size: 1977
Fragments arranged by size:
1977 1452 639 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 5281
BtgI C'CryG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 3358
Fragments arranged by size:
3358 1371 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4542
Fragments arranged by size:
4542 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3591 3611 2797
Size: 794 20 4467
Fragments arranged by size:
4467 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5246
DraI TTT'AAA
Cuts at: 2585 2585
Size: 5281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4288 809
Size: 507 2972 1802
Fragments arranged by size:
2972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2728 3617 5246
Size: 54 1425 50 275 18 898 889 1629
Cuts at: 5246 8
Size: 43
Fragments arranged by size:
1629 1425 898 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2728 1537
Size: 275 916 4090
Fragments arranged by size:
4090 916 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 2555 3211 1353
Size: 1196 6 656 3423
Fragments arranged by size:
3423 1196 656 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3868 4242 4388 387
Size: 1064 138 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2411 2489 3344 3941 4728 4996
Size: 393 480 299 78 855 597 787 268
Cuts at: 4996 1239
Size: 1524
Fragments arranged by size:
1524 855 787 597 480 393 299 268
78
EcoRI G'AATT_C
Cuts at: 2590 2968 2590
Size: 378 4903
FspI TGC'GCA
Cuts at: 3787 3787
Size: 5281
HaeII r_GCGC'y
Cuts at: 1678 2483 4428 1678
Size: 805 1945 2531
Fragments arranged by size:
2531 1945 805
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 3922 3954 3996
Size: 32 135 32 513 32 1805 32 42
Cuts at: 3996 4028 1373
Size: 32 2626
Fragments arranged by size:
2626 1805 513 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2871 669
Size: 988 281 687 246 3079
Fragments arranged by size:
3079 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5281
HpaI GTT'AAC
Cuts at: 2871 2871
Size: 5281
KpnI G_GTAC'C
Cuts at: 2606 2606
Size: 5281
MfeI C'AATT_G
Cuts at: 2880 2880
Size: 5281
MluI A'CGCG_T
Cuts at: 5157 5157
Size: 5281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4395 4579 1305
Size: 3090 184 2007
Fragments arranged by size:
3090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3459
Fragments arranged by size:
3459 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 3239 3598 3757 518
Size: 648 1173 900 359 159 2042
Fragments arranged by size:
2042 1173 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2697 3374 4522 4767 1606
Size: 574 517 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 574 517 245
NcoI C'CATG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 3358
Fragments arranged by size:
3358 1371 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5281
NotI GC'GGCC_GC
Cuts at: 2728 2728
Size: 5281
NsiI A_TGCA'T
Cuts at: 2086 2086
Size: 5281
NspI r_CATG'y
Cuts at: 1132 1229 2639 4184 1132
Size: 97 1410 1545 2229
Fragments arranged by size:
2229 1545 1410 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5281
PciI A'CATG_T
Cuts at: 4180 4180
Size: 5281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5281
PfoI T'CCnGG_A
Cuts at: 2089 2089
Size: 5281
PmeI GTTT'AAAC
Cuts at: 2585 2585
Size: 5281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4863 4895 3350
Size: 32 1481 32 3736
Fragments arranged by size:
3736 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5281
PsiI TTA'TAA
Cuts at: 2851 2851
Size: 5281
PstI C_TGCA'G
Cuts at: 830 2633 830
Size: 1803 3478
PvuI CG_AT'CG
Cuts at: 2001 2382 2001
Size: 381 4900
PvuII CAG'CTG
Cuts at: 2180 2180
Size: 5281
SalI G'TCGA_C
Cuts at: 1936 2623 1936
Size: 687 4594
SbfI CC_TGCA'GG
Cuts at: 2633 2633
Size: 5281
ScaI AGT'ACT
Cuts at: 3529 5241 3529
Size: 1712 3569
SfcI C'TryA_G
Cuts at: 826 1045 2043 2629 3764 4445 4636 826
Size: 219 998 586 1135 681 191 1471
Fragments arranged by size:
1471 1135 998 681 586 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5281
SmaI CCC'GGG
Cuts at: 1872 2608 1872
Size: 736 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 3385 4286 4548 820
Size: 197 926 555 887 901 262 1553
Fragments arranged by size:
1553 926 901 887 555 262 197
SnaBI TAC'GTA
Cuts at: 493 3072 493
Size: 2579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5281
SphI G_CATG'C
Cuts at: 1132 2639 1132
Size: 1507 3774
SspI AAT'ATT
Cuts at: 5 52 3205 5
Size: 47 3153 2081
Fragments arranged by size:
3153 2081 47
StuI AGG'CCT
Cuts at: 1517 2496 1517
Size: 979 4302
StyI C'CwwG_G
Cuts at: 513 1065 2436 2720 513
Size: 552 1371 284 3074
Fragments arranged by size:
3074 1371 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3308 3325 3478 3663 4108 325
Size: 1255 1728 17 153 185 445 1498
Fragments arranged by size:
1728 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3527 5239 96
Size: 274 80 33 51 1443 1550 1712 138
Fragments arranged by size:
1712 1550 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 5241 5273 2139
Size: 32 3070 32 2147
Fragments arranged by size:
3070 2147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5281
XbaI T'CTAG_A
Cuts at: 2617 2617
Size: 5281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2338 2020
Size: 318 4963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5281
XmaI C'CCGG_G
Cuts at: 1870 2606 1870
Size: 736 4545
XmnI GAAnn'nnTTC
Cuts at: 3410 3410
Size: 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3089 276
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI