Restriction map for
pFN21AB8252
(Circular) (Six-base) MAPSORT of: pFN21AB8252.seq Check: 2052 from: 1 to: 5710
pFN21AB8252 5710 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 5157 5425 1632
Size: 2141 1384 268 1917
Fragments arranged by size:
2141 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2139 2721 4609 5586 1203
Size: 936 582 1888 977 1327
Fragments arranged by size:
1888 1327 977 936 582
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 2745 4439 2745
Size: 1694 4016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaI G_GGCC'C
Cuts at: 2810 2810
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2168 2954 3019 3245 3397 5506 1493
Size: 675 786 65 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 675 226 152 65
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2494 2527 2494
Size: 33 5677
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2177 3031 4486 618
Size: 325 217 627 262 128 854 1455 1842
Fragments arranged by size:
1842 1455 854 627 325 262 217 128
BanII G_rGCy'C
Cuts at: 1391 2335 2611 2810 3029 1391
Size: 944 276 199 219 4072
Fragments arranged by size:
4072 944 276 219 199
BbeI G_GCGC'C
Cuts at: 2053 2053
Size: 5710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 2567 928
Size: 971 400 268 4071
Fragments arranged by size:
4071 971 400 268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2996 4321 136
Size: 107 122 71 631 605 1324 1325 1525
Fragments arranged by size:
1525 1325 1324 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 2473 3127 2473
Size: 654 5056
Bme1580I G_kGCm'C
Cuts at: 1790 2810 3771 4927 1790
Size: 1020 961 1156 2573
Fragments arranged by size:
2573 1156 1020 961
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2597 4370 1239
Size: 1358 1773 2579
Fragments arranged by size:
2579 1773 1358
Bpu10I CC'TnA_GC
Cuts at: 2670 2670
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2798 3835 4700 4998 2420
Size: 378 1037 865 298 3132
Fragments arranged by size:
3132 1037 865 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2854 4373 882
Size: 617 1355 1519 2219
Fragments arranged by size:
2219 1519 1355 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 2304 2798 3517
Size: 53 83 186 936 517 254 494 719
Cuts at: 3517 3899 4534 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 719 635 517 494 382 254
186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2861 2891 2984 3014 1178
Size: 30 165 30 1458 30 93 30 3874
Fragments arranged by size:
3874 1458 165 93 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2842 2869 1186
Size: 1656 27 4027
Fragments arranged by size:
4027 1656 27
BseYI C'CCAG_C
Cuts at: 2327 2894 4913 5409 2327
Size: 567 2019 496 2628
Fragments arranged by size:
2628 2019 567 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2443 1163
Size: 573 707 4430
Fragments arranged by size:
4430 707 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2841 3771 3856 4927 2841
Size: 930 85 1071 3624
Fragments arranged by size:
3624 1071 930 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2335 2611 2810 2841 3029 3771 3856
Size: 399 545 276 199 31 188 742 85
Cuts at: 3856 4927 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 276 199 188
85 31
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 2526 3599 5329 2526
Size: 1073 1730 2907
Fragments arranged by size:
2907 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2750 2767 3065 844
Size: 1906 17 298 3489
Fragments arranged by size:
3489 1906 298 17
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2455 2904 4205 4379 65
Size: 1183 1207 449 1301 174 1396
Fragments arranged by size:
1396 1301 1207 1183 449 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 2056 1184
Size: 872 4838
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 2653
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2245 3040 3407 3792 3809 5261 5347 5359
Size: 273 795 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 795 385 367 346 273 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2161 2173 2316 2602 513
Size: 552 939 157 12 143 286 3621
Fragments arranged by size:
3621 939 552 286 157 143 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2283 487
Size: 683 56 1057 3914
Fragments arranged by size:
3914 1057 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 2712 3226 4020 4040 2547
Size: 165 514 794 20 4217
Fragments arranged by size:
4217 794 514 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DraIII CAC_nnn'GTG
Cuts at: 2845 2845
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2924 4717 809
Size: 507 1608 1793 1802
Fragments arranged by size:
1802 1793 1608 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 2599 3157
Size: 54 1425 50 275 18 654 115 558
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 654 558 275 115 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2597 3773 4370 5157 5425 1239
Size: 393 965 1176 597 787 268 1524
Fragments arranged by size:
1524 1176 965 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2486
Size: 5710
EcoO109I rG'GnC_Cy
Cuts at: 2806 2807 2806
Size: 1 5709
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2053 2280 2643 4857 1678
Size: 375 227 363 2214 2531
Fragments arranged by size:
2531 2214 375 363 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 2731 2763 2861
Size: 32 135 32 989 32 138 32 98
Cuts at: 2861 2893 4351 4383 4425 4457 1373
Size: 32 1458 32 42 32 2626
Fragments arranged by size:
2626 1458 989 138 135 98 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 3054 3300 669
Size: 988 281 91 1025 246 3079
Fragments arranged by size:
3079 1025 988 281 246 91
HindIII A'AGCT_T
Cuts at: 748 2201 748
Size: 1453 4257
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2049 2049
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2002 2781 4824 5008 1305
Size: 697 779 2043 184 2007
Fragments arranged by size:
2043 2007 779 697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 10
Size: 54 1425 343 654 3234
Fragments arranged by size:
3234 1425 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2405 2693 3668 4027 4186 518
Size: 648 1149 90 288 975 359 159 2042
Fragments arranged by size:
2042 1149 975 648 359 288 159 90
MspA1I CmG'CkG
Cuts at: 1606 2604 3126 3803 4951 5196 1606
Size: 998 522 677 1148 245 2120
Fragments arranged by size:
2120 1148 998 677 522 245
NarI GG'CG_CC
Cuts at: 2050 2050
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 2004 2161 2173 2316 513
Size: 552 939 157 12 143 3907
Fragments arranged by size:
3907 939 552 157 143 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2143 3068 4613 1132
Size: 97 914 925 1545 2229
Fragments arranged by size:
2229 1545 925 914 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 2139 4609 2139
Size: 2470 3240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PfoI T'CCnGG_A
Cuts at: 2235 2579 2235
Size: 344 5366
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2534 1613
Size: 921 4789
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2806 2806
Size: 5710
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SacII CC_GC'GG
Cuts at: 2605 2605
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SexAI A'CCwGG_T
Cuts at: 2841 2841
Size: 5710
SfcI C'TryA_G
Cuts at: 826 1045 2962 3058 4193 4874 5065 826
Size: 219 1917 96 1135 681 191 1471
Fragments arranged by size:
1917 1471 1135 681 219 191 96
SfiI GGCCn_nnn'nGGCC
Cuts at: 2996 2996
Size: 5710
SfoI GGC'GCC
Cuts at: 2051 2051
Size: 5710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 2813 3814 4715 4977 820
Size: 197 926 492 378 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 492 378 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2173 2316 2487 2646 3003
Size: 552 939 157 12 143 171 159 357
Cuts at: 3003 3149 513
Size: 146 3074
Fragments arranged by size:
3074 939 552 357 171 159 157 146
143 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 2393 2570 2888 3737 3754 3907
Size: 1255 594 219 177 318 849 17 153
Cuts at: 3907 4092 4537 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 849 594 445 318 219 185
177 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 5670 5702 2984
Size: 32 2654 32 2992
Fragments arranged by size:
2992 2654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 2749 3046 2749
Size: 297 5413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2504 2725 2398
Size: 106 221 5383
Fragments arranged by size:
5383 221 106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SrfI
SwaI