Restriction map for
pFN21AB8242
(Circular) (Six-base) MAPSORT of: pFN21AB8242.seq Check: 4695 from: 1 to: 5299
pFN21AB8242 5299 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 278
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2364 2642 1937
Size: 427 278 4594
Fragments arranged by size:
4594 427 278
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 3426 3799 1563
Size: 1863 373 3063
Fragments arranged by size:
3063 1863 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 4746 5014 1632
Size: 1730 1384 268 1917
Fragments arranged by size:
1917 1730 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5102
AflIII A'CryG_T
Cuts at: 1203 4198 5175 1203
Size: 2995 977 1327
Fragments arranged by size:
2995 1327 977
AgeI A'CCGG_T
Cuts at: 4130 4130
Size: 5299
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2169 1184
Size: 985 4314
AlwNI CAG_nnn'CTG
Cuts at: 3042 4614 3042
Size: 1572 3727
ApaLI G'TGCA_C
Cuts at: 3356 4512 3356
Size: 1156 4143
ApoI r'AATT_y
Cuts at: 1493 2285 2428 2557 2608 2834 2986 5095 1493
Size: 792 143 129 51 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 226 152 143 129 51
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2624 1870
Size: 73 71 610 4545
Fragments arranged by size:
4545 610 73 71
BamHI G'GATC_C
Cuts at: 2101 2629 2996 2101
Size: 528 367 4404
Fragments arranged by size:
4404 528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2620 4075 618
Size: 325 217 627 240 593 1455 1842
Fragments arranged by size:
1842 1455 627 593 325 240 217
BanII G_rGCy'C
Cuts at: 1391 2068 2509 2618 1391
Size: 677 441 109 4072
Fragments arranged by size:
4072 677 441 109
BbeI G_GCGC'C
Cuts at: 2031 2031
Size: 5299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2533 928
Size: 971 634 3694
Fragments arranged by size:
3694 971 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3490 3524 1903
Size: 34 1553 34 3678
Fragments arranged by size:
3678 1553 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2195 2527 3191 4401 2195
Size: 332 664 1210 3093
Fragments arranged by size:
3093 1210 664 332
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3910 136
Size: 107 122 71 631 605 2238 1525
Fragments arranged by size:
2238 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5294 5294
Size: 5299
BlpI GC'TnA_GC
Cuts at: 2541 2716 2541
Size: 175 5124
Bme1580I G_kGCm'C
Cuts at: 1790 3360 4516 1790
Size: 1570 1156 2573
Fragments arranged by size:
2573 1570 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3988 442
Size: 416 53 83 399 24 1613 958 1753
Fragments arranged by size:
1753 1613 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2240 2338 3959 1239
Size: 978 23 98 1621 2579
Fragments arranged by size:
2579 1621 978 98 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3424 4289 4587 3424
Size: 865 298 4136
Fragments arranged by size:
4136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3962 882
Size: 617 2463 2219
Fragments arranged by size:
2463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3090 493
Size: 711 1886 2702
Fragments arranged by size:
2702 1886 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 3106 3488 4123
Size: 53 83 186 936 495 1078 382 635
Cuts at: 4123 275
Size: 1451
Fragments arranged by size:
1451 1078 936 635 495 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3732 4130 4404 4551 4962 3732
Size: 398 274 147 411 4069
Fragments arranged by size:
4069 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5074
Fragments arranged by size:
5074 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5299
BseYI C'CCAG_C
Cuts at: 4502 4998 4502
Size: 496 4803
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4726
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2749 3510 4538 1540
Size: 275 186 748 761 1028 2301
Fragments arranged by size:
2301 1028 761 748 275 186
BsiHKAI G_wGCw'C
Cuts at: 3360 3445 4516 3360
Size: 85 1071 4143
Fragments arranged by size:
4143 1071 85
BsmI GAATG_Cn'
Cuts at: 2810 2903 2810
Size: 93 5206
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2509 2618 3360 3445 4516 1391
Size: 399 278 441 109 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 278 109 85
BspEI T'CCGG_A
Cuts at: 4962 4962
Size: 5299
BspHI T'CATG_A
Cuts at: 2589 3188 4918 2589
Size: 599 1730 2970
Fragments arranged by size:
2970 1730 599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 844
Size: 1810 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 1394
Size: 429 1363 3507
Fragments arranged by size:
3507 1363 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2190 3794 3968 65
Size: 1183 942 1604 174 1396
Fragments arranged by size:
1604 1396 1183 942 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2229 3943 4130 1700
Size: 110 220 199 1714 187 2869
Fragments arranged by size:
2869 1714 220 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5299
BssSI C'ACGA_G
Cuts at: 3359 4371 3359
Size: 1012 4287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5299
BstBI TT'CG_AA
Cuts at: 2524 4103 2524
Size: 1579 3720
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2101 2629 2996 3381 3398 4850 4936 4948
Size: 129 528 367 385 17 1452 86 12
Cuts at: 4948 5294 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 528 385 367 346 129 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3808
Fragments arranged by size:
3808 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2467 487
Size: 683 56 1241 3319
Fragments arranged by size:
3319 1241 683 56
BtsI GCAGTG_nn'
Cuts at: 2815 3609 3629 2815
Size: 794 20 4485
Fragments arranged by size:
4485 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2358 2393 529
Size: 35 1794 35 3435
Fragments arranged by size:
3435 1794 35 35
DraI TTT'AAA
Cuts at: 2290 2603 2290
Size: 313 4986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4306 809
Size: 507 2990 1802
Fragments arranged by size:
2990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2746 3635 5264
Size: 54 1425 50 275 18 916 889 1629
Cuts at: 5264 8
Size: 43
Fragments arranged by size:
1629 1425 916 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2746 1537
Size: 275 934 4090
Fragments arranged by size:
4090 934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 1353
Size: 1876 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3886 4260 4406 387
Size: 1064 138 2297 374 146 1280
Fragments arranged by size:
2297 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2240 2338 3362 3959 4746 5014
Size: 393 585 23 98 1024 597 787 268
Cuts at: 5014 1239
Size: 1524
Fragments arranged by size:
1524 1024 787 597 585 393 268 98
23
EcoO109I rG'GnC_Cy
Cuts at: 2075 2386 2075
Size: 311 4988
EcoRI G'AATT_C
Cuts at: 2428 2608 2986 2428
Size: 180 378 4741
Fragments arranged by size:
4741 378 180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 5299
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 2031 4446 1678
Size: 353 2415 2531
Fragments arranged by size:
2531 2415 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3940 3972 4014 4046 1373
Size: 32 135 32 2368 32 42 32 2626
Fragments arranged by size:
2626 2368 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5299
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KasI G'GCGC_C
Cuts at: 2027 2027
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2433 2898 2433
Size: 465 4834
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4413 4597 1305
Size: 3108 184 2007
Fragments arranged by size:
3108 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3477
Fragments arranged by size:
3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3616 3775 518
Size: 648 2091 359 159 2042
Fragments arranged by size:
2091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2715 3392 4540 4785 1606
Size: 501 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 501 245
NaeI GCC'GGC
Cuts at: 2032 2231 2032
Size: 199 5100
NarI GG'CG_CC
Cuts at: 2028 2028
Size: 5299
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3808
Fragments arranged by size:
3808 939 552
NdeI CA'TA_TG
Cuts at: 387 2566 387
Size: 2179 3120
NgoMIV G'CCGG_C
Cuts at: 2030 2229 2030
Size: 199 5100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5299
NotI GC'GGCC_GC
Cuts at: 2746 2746
Size: 5299
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5299
PfoI T'CCnGG_A
Cuts at: 2068 2086 2068
Size: 18 5281
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5299
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2651 830
Size: 1821 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2127 2647 3782 4463 4654 826
Size: 219 1082 520 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 681 520 219 191
SfoI GGC'GCC
Cuts at: 2029 2029
Size: 5299
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 2030 1700
Size: 330 4969
SmaI CCC'GGG
Cuts at: 1872 2016 2626 1872
Size: 144 610 4545
Fragments arranged by size:
4545 610 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3403 4304 4566 820
Size: 197 926 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5299
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SspI AAT'ATT
Cuts at: 5 52 3223 5
Size: 47 3171 2081
Fragments arranged by size:
3171 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5299
StyI C'CwwG_G
Cuts at: 513 1065 2004 2738 513
Size: 552 939 734 3074
Fragments arranged by size:
3074 939 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3326 3343 3496 3681 4126 325
Size: 1255 1746 17 153 185 445 1498
Fragments arranged by size:
1746 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2305 3545 5257
Size: 274 80 33 51 1443 328 1240 1712
Cuts at: 5257 96
Size: 138
Fragments arranged by size:
1712 1443 1240 328 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5259 5291 5259
Size: 32 5267
Tth111I GACn'n_nGTC
Cuts at: 1316 2182 1316
Size: 866 4433
XbaI T'CTAG_A
Cuts at: 2635 2635
Size: 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2014 2624 1870
Size: 144 610 4545
Fragments arranged by size:
4545 610 144
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 276
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI