Restriction map for
pFN21AB8238
(Circular) (Six-base) MAPSORT of: pFN21AB8238.seq Check: 3128 from: 1 to: 5596
pFN21AB8238 5596 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2575 2575
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2504 2594 3659 5043 5311 1632
Size: 695 177 90 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 695 268 177 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 2585 1166
Size: 1419 4177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2450 1184
Size: 1266 4330
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 2336 2368 2108
Size: 32 196 32 5336
Fragments arranged by size:
5336 196 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2251 2260 2533 3339 4911 2251
Size: 9 273 806 1572 2936
Fragments arranged by size:
2936 1572 806 273 9
ApaLI G'TGCA_C
Cuts at: 2084 3653 4809 2084
Size: 1569 1156 2871
Fragments arranged by size:
2871 1569 1156
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2228 2921 1870
Size: 73 146 139 693 4545
Fragments arranged by size:
4545 693 146 139 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 4372 618
Size: 325 217 627 1130 1455 1842
Fragments arranged by size:
1842 1455 1130 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2114 2322 2415 2747 2915 1391
Size: 723 208 93 332 168 4072
Fragments arranged by size:
4072 723 332 208 168 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2573 2607 3787 3821 1903
Size: 34 50 34 552 34 1180 34 3678
Fragments arranged by size:
3678 1180 552 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 2715 1548
Size: 1167 4429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2610 5591 2610
Size: 2981 2615
BlpI GC'TnA_GC
Cuts at: 2746 3013 2746
Size: 267 5329
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2096 3657 4813 1790
Size: 298 8 1561 1156 2573
Fragments arranged by size:
2573 1561 1156 298 8
BmgBI CAC'GTC
Cuts at: 1318 2779 1318
Size: 1461 4135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 3327 4285
Size: 416 53 83 399 24 760 1150 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1150 958 760 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 4256 1239
Size: 1574 1443 2579
Fragments arranged by size:
2579 1574 1443
Bpu10I CC'TnA_GC
Cuts at: 2741 2741
Size: 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2758 3387 493
Size: 711 1554 629 2702
Fragments arranged by size:
2702 1554 711 629
BsaBI GATnn'nnATC
Cuts at: 2834 5590 2834
Size: 2756 2840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2880 3403 3785 4420
Size: 53 83 186 936 1347 523 382 635
Cuts at: 4420 275
Size: 1451
Fragments arranged by size:
1451 1347 936 635 523 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2139 4029 4427 4701 4848 5259 2139
Size: 1890 398 274 147 411 2476
Fragments arranged by size:
2476 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 1178
Size: 30 165 30 933 30 4408
Fragments arranged by size:
4408 933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2246 2531 1186
Size: 949 111 285 4251
Fragments arranged by size:
4251 949 285 111
BseYI C'CCAG_C
Cuts at: 2104 2281 2379 2397 2498 4799 5295 2104
Size: 177 98 18 101 2301 496 2405
Fragments arranged by size:
2405 2301 496 177 101 98 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 1163
Size: 573 912 4111
Fragments arranged by size:
4111 912 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2415 2747 2778 3657 3742 4813 2088
Size: 327 332 31 879 85 1071 2871
Fragments arranged by size:
2871 1071 879 332 327 85 31
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2096 2114 2322 2415 2747 2778
Size: 399 298 8 18 208 93 332 31
Cuts at: 2778 2915 3657 3742 4813 1391
Size: 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 298 208 137
93 85 31 18 8
BspEI T'CCGG_A
Cuts at: 2139 5259 2139
Size: 3120 2476
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2191 2268 2575 2951 844
Size: 1347 77 307 376 3489
Fragments arranged by size:
3489 1347 376 307 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2467 3483 1394
Size: 429 245 399 1016 3507
Fragments arranged by size:
3507 1016 429 399 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 2021 3656 4668 2021
Size: 1635 1012 2949
Fragments arranged by size:
2949 1635 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2251 2260 794
Size: 1457 9 4130
Fragments arranged by size:
4130 1457 9
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 2203 1184
Size: 1019 4577
BstYI r'GATC_y
Cuts at: 1972 2292 2610 2631 2926 3293 3678 3695 5147
Size: 320 318 21 295 367 385 17 1452
Cuts at: 5147 5233 5245 5591 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 320 318 295
86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5044
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2280 487
Size: 683 56 1054 3803
Fragments arranged by size:
3803 1054 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 3043 3932
Size: 54 1425 50 275 18 384 829 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 829 384 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 3526 1353
Size: 696 1477 3423
Fragments arranged by size:
3423 1477 696
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 4183 4557 4703 387
Size: 1064 138 696 1898 374 146 1280
Fragments arranged by size:
1898 1280 1064 696 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2745 2413
Size: 332 5264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2504 2594 2813 3659 4256 5043
Size: 393 695 177 90 219 846 597 787
Cuts at: 5043 5311 1239
Size: 268 1524
Fragments arranged by size:
1524 846 787 695 597 393 268 219
177 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 2228
Size: 5596
EcoO109I rG'GnC_Cy
Cuts at: 2016 2389 2016
Size: 373 5223
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 5596
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2704 2736 4237
Size: 32 135 32 458 32 642 32 1501
Cuts at: 4237 4269 4311 4343 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1501 642 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2359 2940 3186 669
Size: 988 281 421 581 246 3079
Fragments arranged by size:
3079 988 581 421 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2502 2872 4710 4894 1305
Size: 864 333 370 1838 184 2007
Fragments arranged by size:
2007 1838 864 370 333 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 3390
Fragments arranged by size:
3390 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2366 2585 2840 3554 3913 4072 518
Size: 648 1200 219 255 714 359 159 2042
Fragments arranged by size:
2042 1200 714 648 359 255 219 159
MspA1I CmG'CkG
Cuts at: 1606 2281 2305 2482 2750 3012 3689 4837 5082
Size: 675 24 177 268 262 677 1148 245
Cuts at: 5082 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 675 268 262 245 177
24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 1108
Size: 1080 4516
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2336 2368 3665 3697 5178 5210 2336
Size: 32 1297 32 1481 32 2722
Fragments arranged by size:
2722 1481 1297 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2389 2016
Size: 373 5223
PshAI GACnn'nnGTC
Cuts at: 1613 2159 1613
Size: 546 5050
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2256 2304 2948 830
Size: 1426 48 644 3478
Fragments arranged by size:
3478 1426 644 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2281 2305 2482 2281
Size: 24 177 5395
Fragments arranged by size:
5395 177 24
SacI G_AGCT'C
Cuts at: 2415 2747 2415
Size: 332 5264
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2252 2300 2944 4079 4760 4951 826
Size: 219 1207 48 644 1135 681 191 1471
Fragments arranged by size:
1471 1207 1135 681 644 219 191 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2091 2923 1872
Size: 219 832 4545
Fragments arranged by size:
4545 832 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 1517
Size: 1083 4513
StyI C'CwwG_G
Cuts at: 513 1065 3035 513
Size: 552 1970 3074
Fragments arranged by size:
3074 1970 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2209 2241 5556 5588 2209
Size: 32 3315 32 2217
Fragments arranged by size:
3315 2217 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2500 2500
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2089 2921 1870
Size: 219 832 4545
Fragments arranged by size:
4545 832 219
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI