Restriction map for
pFN21AB8234
(Circular) (Six-base) MAPSORT of: pFN21AB8234.seq Check: 3543 from: 1 to: 5479
pFN21AB8234 5479 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2601 2800 2601
Size: 199 5280
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2518 3542 4926 5194 1632
Size: 886 1024 1384 268 1917
Fragments arranged by size:
1917 1384 1024 886 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 2282 4378 5355 1203
Size: 1079 2096 977 1327
Fragments arranged by size:
2096 1327 1079 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 2250 1166
Size: 1084 4395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 3222 4794 3222
Size: 1572 3907
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 1870
Size: 73 861 4545
Fragments arranged by size:
4545 861 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2383 2416 2521 2554 2383
Size: 33 105 33 5308
Fragments arranged by size:
5308 105 33 33
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2601 2800 4255 618
Size: 325 217 627 814 199 1455 1842
Fragments arranged by size:
1842 1455 814 627 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2169 2798 1391
Size: 778 629 4072
Fragments arranged by size:
4072 778 629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2285 928
Size: 971 386 4122
Fragments arranged by size:
4122 971 386
BbvCI CC'TCA_GC
Cuts at: 2033 2033
Size: 5479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4090 136
Size: 107 122 71 631 605 337 2081 1525
Fragments arranged by size:
2081 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 3540 4696 1790
Size: 1750 1156 2573
Fragments arranged by size:
2573 1750 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3210 4168
Size: 416 53 83 399 24 1343 450 958
Cuts at: 4168 442
Size: 1753
Fragments arranged by size:
1753 1343 958 450 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2580 2580
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 4139 1239
Size: 836 2064 2579
Fragments arranged by size:
2579 2064 836
Bpu10I CC'TnA_GC
Cuts at: 2033 2033
Size: 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 2590 4142 882
Size: 617 993 98 1552 2219
Fragments arranged by size:
2219 1552 993 617 98
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3286 3668 4303
Size: 53 83 186 936 479 1274 382 635
Cuts at: 4303 275
Size: 1451
Fragments arranged by size:
1451 1274 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2629 3912 4310 4584 4731 5142 2629
Size: 1283 398 274 147 411 2966
Fragments arranged by size:
2966 1283 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 2418 2448 1178
Size: 30 165 30 692 30 293 30 4209
Fragments arranged by size:
4209 692 293 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2734 1186
Size: 1294 254 3931
Fragments arranged by size:
3931 1294 254
BseYI C'CCAG_C
Cuts at: 4682 5178 4682
Size: 496 4983
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4906
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2776 3540 3625 4696 2776
Size: 764 85 1071 3559
Fragments arranged by size:
3559 1071 764 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2776 2798 3540 3625 4696 1391
Size: 399 379 607 22 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 607 399 379 85 22
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2834 844
Size: 1990 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 4123 4310 1700
Size: 110 845 1468 187 2869
Fragments arranged by size:
2869 1468 845 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 2591 1184
Size: 1407 4072
BstXI CCAn_nnnn'nTGG
Cuts at: 2252 2252
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2519 2606 513
Size: 552 939 515 87 3386
Fragments arranged by size:
3386 939 552 515 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3789 3809 2995
Size: 794 20 4665
Fragments arranged by size:
4665 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2676 3409 1353
Size: 710 613 733 3423
Fragments arranged by size:
3423 733 710 613
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2556 4066 4440 4586 387
Size: 1064 138 967 1510 374 146 1280
Fragments arranged by size:
1510 1280 1064 967 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2518 3542 4139 4926 5194 1239
Size: 393 443 443 1024 597 787 268 1524
Fragments arranged by size:
1524 1024 787 597 443 443 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2524 2762 2524
Size: 238 5241
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 4626 1678
Size: 2948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2749 2781 4120
Size: 32 135 32 545 32 600 32 1339
Cuts at: 4120 4152 4194 4226 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1339 600 545 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 2454 2823 3069 669
Size: 988 281 412 104 369 246 3079
Fragments arranged by size:
3079 988 412 369 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2605 2804 2605
Size: 199 5280
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2273 4593 4777 1305
Size: 968 2320 184 2007
Fragments arranged by size:
2320 2007 968 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2250 3437 3796 3955 518
Size: 648 1084 1187 359 159 2042
Fragments arranged by size:
2042 1187 1084 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2735 2895 3572 4720 4965 1606
Size: 663 466 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 466 245 160
NaeI GCC'GGC
Cuts at: 2657 2657
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2004 2606 513
Size: 552 939 602 3386
Fragments arranged by size:
3386 939 602 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NgoMIV G'CCGG_C
Cuts at: 2655 2655
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2286 2348 2837 4382 1132
Size: 97 1057 62 489 1545 2229
Fragments arranged by size:
2229 1545 1057 489 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 2282 4378 2282
Size: 2096 3383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5479
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2067 2099 3548 3580 5061 5093 2067
Size: 32 1449 32 1481 32 2453
Fragments arranged by size:
2453 1481 1449 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2524
Size: 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 2157 3049 2157
Size: 892 4587
PstI C_TGCA'G
Cuts at: 830 2831 830
Size: 2001 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2827 3962 4643 4834 826
Size: 219 1782 1135 681 191 1471
Fragments arranged by size:
1782 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2004 2606 2918 513
Size: 552 939 602 312 3074
Fragments arranged by size:
3074 939 602 552 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 2418 2450 5439 5471 2095
Size: 32 291 32 2989 32 2103
Fragments arranged by size:
2989 2103 291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2613 2684 2204
Size: 409 71 4999
Fragments arranged by size:
4999 409 71
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI