Restriction map for
pFN21AB8231
(Circular) (Six-base) MAPSORT of: pFN21AB8231.seq Check: 2353 from: 1 to: 5338
pFN21AB8231 5338 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2340 2659 2340
Size: 319 5019
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3401 4785 5053 1632
Size: 602 1167 1384 268 1917
Fragments arranged by size:
1917 1384 1167 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 2131 2575 3081 4653 2131
Size: 444 506 1572 2816
Fragments arranged by size:
2816 1572 506 444
ApaI G_GGCC'C
Cuts at: 2216 2216
Size: 5338
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2663 1870
Size: 73 229 491 4545
Fragments arranged by size:
4545 491 229 73
BamHI G'GATC_C
Cuts at: 2629 2668 3035 2629
Size: 39 367 4932
Fragments arranged by size:
4932 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2340 2659 4114 618
Size: 325 217 627 553 319 1455 1842
Fragments arranged by size:
1842 1455 627 553 325 319 217
BanII G_rGCy'C
Cuts at: 1391 2216 2239 2657 1391
Size: 825 23 418 4072
Fragments arranged by size:
4072 825 418 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3529 3563 1903
Size: 34 1592 34 3678
Fragments arranged by size:
3678 1592 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2216 3399 4555 1790
Size: 426 1183 1156 2573
Fragments arranged by size:
2573 1183 1156 426
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1996 2055 2322 3998 1239
Size: 757 59 267 1676 2579
Fragments arranged by size:
2579 1676 757 267 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 2026 2113 4001 882
Size: 617 515 12 87 1888 2219
Fragments arranged by size:
2219 1888 617 515 87 12
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 2628 5332 2628
Size: 2704 2634
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3145 3527 4162 275
Size: 53 83 186 936 1612 382 635 1451
Fragments arranged by size:
1612 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2458 3771 4169 4443 4590 5001 2458
Size: 1313 398 274 147 411 2795
Fragments arranged by size:
2795 1313 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2538 2557 1186
Size: 1352 19 3967
Fragments arranged by size:
3967 1352 19
BseYI C'CCAG_C
Cuts at: 4541 5037 4541
Size: 496 4842
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2239 3399 3484 4555 2239
Size: 1160 85 1071 3022
Fragments arranged by size:
3022 1160 1071 85
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
BsmBI CGTCTCn'nnnn_
Cuts at: 2524 2524
Size: 5338
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2239 2657 3399 3484 4555 1391
Size: 399 426 23 418 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 418 399 85 23
BspEI T'CCGG_A
Cuts at: 2458 5001 2458
Size: 2543 2795
BspHI T'CATG_A
Cuts at: 2239 3227 4957 2239
Size: 988 1730 2620
Fragments arranged by size:
2620 1730 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2160 3833 4007 65
Size: 1183 912 1673 174 1396
Fragments arranged by size:
1673 1396 1183 912 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3982 4169 1700
Size: 110 2172 187 2869
Fragments arranged by size:
2869 2172 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2629 2668 3035 3420 3437 4889 4975 4987
Size: 657 39 367 385 17 1452 86 12
Cuts at: 4987 5333 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 657 385 367 346 86 39
17 12
Bsu36I CC'TnA_GG
Cuts at: 2316 2316
Size: 5338
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4599
Fragments arranged by size:
4599 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 2854 3648 3668 2409
Size: 445 794 20 4079
Fragments arranged by size:
4079 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2785 3674 5303
Size: 54 1425 50 275 18 955 889 1629
Cuts at: 5303 8
Size: 43
Fragments arranged by size:
1629 1425 955 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 1353
Size: 1915 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3925 4299 4445 387
Size: 1064 138 2336 374 146 1280
Fragments arranged by size:
2336 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2237 2237
Size: 5338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2055 2234 2322 3401 3998 4785
Size: 393 364 59 179 88 1079 597 787
Cuts at: 4785 5053 1239
Size: 268 1524
Fragments arranged by size:
1524 1079 787 597 393 364 268 179
88 59
EcoO109I rG'GnC_Cy
Cuts at: 2169 2213 2261 2169
Size: 44 48 5246
Fragments arranged by size:
5246 48 44
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
EcoRV GAT'ATC
Cuts at: 2122 2122
Size: 5338
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 4485 1678
Size: 2807 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2536 2568 3979
Size: 32 135 32 521 32 411 32 1411
Cuts at: 3979 4011 4053 4085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1411 521 411 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2298 2682 2928 669
Size: 988 281 360 384 246 3079
Fragments arranged by size:
3079 988 384 360 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2344 2663 2344
Size: 319 5019
MfeI C'AATT_G
Cuts at: 2153 2937 2153
Size: 784 4554
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2350 4452 4636 1305
Size: 1045 2102 184 2007
Fragments arranged by size:
2102 2007 1045 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2062 3296 3655 3814 518
Size: 648 896 1234 359 159 2042
Fragments arranged by size:
2042 1234 896 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2754 3431 4579 4824 1606
Size: 1148 677 1148 245 2120
Fragments arranged by size:
2120 1148 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3847
Fragments arranged by size:
3847 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PfoI T'CCnGG_A
Cuts at: 2012 2012
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2261 2261
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspOMI G'GGCC_C
Cuts at: 2212 2212
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2416 2690 830
Size: 1586 274 3478
Fragments arranged by size:
3478 1586 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
SacI G_AGCT'C
Cuts at: 2239 2239
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2412 2686 3821 4502 4693 826
Size: 219 1367 274 1135 681 191 1471
Fragments arranged by size:
1471 1367 1135 681 274 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2777 513
Size: 552 939 773 3074
Fragments arranged by size:
3074 939 773 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 3382 3535 3720 4165 325
Size: 1255 1785 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 2105 1316
Size: 789 4549
XbaI T'CTAG_A
Cuts at: 2392 2674 2392
Size: 282 5056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 2206 2173
Size: 33 5305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 2247 3467 2247
Size: 1220 4118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI