Restriction map for
pFN21AB8228
(Circular) (Six-base) MAPSORT of: pFN21AB8228.seq Check: 6993 from: 1 to: 5932
pFN21AB8228 5932 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3742 278
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3275 1937
Size: 1338 4594
Acc65I G'GTAC_C
Cuts at: 3253 3253
Size: 5932
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3995 5379 5647 1632
Size: 2363 1384 268 1917
Fragments arranged by size:
2363 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 4831 5808 1203
Size: 3628 977 1327
Fragments arranged by size:
3628 1327 977
AgeI A'CCGG_T
Cuts at: 4763 4763
Size: 5932
AhdI GACnn_n'nnGTC
Cuts at: 4661 4661
Size: 5932
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5932
AlwNI CAG_nnn'CTG
Cuts at: 2935 2989 3058 3675 5247 2935
Size: 54 69 617 1572 3620
Fragments arranged by size:
3620 1572 617 69 54
ApaLI G'TGCA_C
Cuts at: 3989 5145 3989
Size: 1156 4776
ApoI r'AATT_y
Cuts at: 1493 2093 3241 3467 3619 5728 1493
Size: 600 1148 226 152 2109 1697
Fragments arranged by size:
2109 1697 1148 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4486 160
Size: 872 3454 1606
Fragments arranged by size:
3454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2400 2466 2908 3257 1870
Size: 73 126 331 66 442 349 4545
Fragments arranged by size:
4545 442 349 331 126 73 66
BamHI G'GATC_C
Cuts at: 2925 3262 3629 2925
Size: 337 367 5228
Fragments arranged by size:
5228 367 337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 2773 3253 4708 618
Size: 325 217 627 536 450 480 1455 1842
Fragments arranged by size:
1842 1455 627 536 480 450 325 217
BanII G_rGCy'C
Cuts at: 1391 2247 3251 1391
Size: 856 1004 4072
Fragments arranged by size:
4072 1004 856
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 928
Size: 971 690 4271
Fragments arranged by size:
4271 971 690
BbvCI CC'TCA_GC
Cuts at: 2877 2877
Size: 5932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4123 4157 1903
Size: 34 2186 34 3678
Fragments arranged by size:
3678 2186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3824 5034 3824
Size: 1210 4722
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5932
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 4543 136
Size: 107 122 71 631 605 985 1886 1525
Fragments arranged by size:
1886 1525 985 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5927 5927
Size: 5932
BlpI GC'TnA_GC
Cuts at: 3349 3349
Size: 5932
Bme1580I G_kGCm'C
Cuts at: 1790 3993 5149 1790
Size: 2203 1156 2573
Fragments arranged by size:
2573 2203 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2999 3663 4621
Size: 416 53 83 399 24 1582 664 958
Cuts at: 4621 442
Size: 1753
Fragments arranged by size:
1753 1582 958 664 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 2849 2972 3083 4592 1239
Size: 1601 9 123 111 1509 2579
Fragments arranged by size:
2579 1601 1509 123 111 9
Bpu10I CC'TnA_GC
Cuts at: 2877 2877
Size: 5932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4057 4922 5220 4057
Size: 865 298 4769
Fragments arranged by size:
4769 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3033 3138 4595 882
Size: 617 1534 105 1457 2219
Fragments arranged by size:
2219 1534 1457 617 105
BsaAI yAC'GTr
Cuts at: 493 1204 3723 493
Size: 711 2519 2702
Fragments arranged by size:
2702 2519 711
BsaBI GATnn'nnATC
Cuts at: 5926 5926
Size: 5932
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2789 3739 4121 4756
Size: 53 83 186 936 1256 950 382 635
Cuts at: 4756 275
Size: 1451
Fragments arranged by size:
1451 1256 950 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2088 2247 3216 4365 4763 5037 5184 5595 2088
Size: 159 969 1149 398 274 147 411 2425
Fragments arranged by size:
2425 1149 969 411 398 274 159 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5707
Fragments arranged by size:
5707 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 2837 2969 1186
Size: 1267 384 132 4149
Fragments arranged by size:
4149 1267 384 132
BseYI C'CCAG_C
Cuts at: 2082 2652 3079 5135 5631 2082
Size: 570 427 2056 496 2383
Fragments arranged by size:
2383 2056 570 496 427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 1163
Size: 573 310 5049
Fragments arranged by size:
5049 573 310
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 3993 4078 5149 2247
Size: 1746 85 1071 3030
Fragments arranged by size:
3030 1746 1071 85
BsmI GAATG_Cn'
Cuts at: 3443 3536 3443
Size: 93 5839
BsmBI CGTCTCn'nnnn_
Cuts at: 3059 3059
Size: 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 3251 3993 4078 5149 1391
Size: 399 457 1004 742 85 1071 2174
Fragments arranged by size:
2174 1071 1004 742 457 399 85
BspEI T'CCGG_A
Cuts at: 2088 2247 5595 2088
Size: 159 3348 2425
Fragments arranged by size:
3348 2425 159
BspHI T'CATG_A
Cuts at: 3821 5551 3821
Size: 1730 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3174 3287 844
Size: 2330 113 3489
Fragments arranged by size:
3489 2330 113
BsrBI CCG'CTC
Cuts at: 1394 1823 2678 3819 1394
Size: 429 855 1141 3507
Fragments arranged by size:
3507 1141 855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4427 4601 65
Size: 1183 3179 174 1396
Fragments arranged by size:
3179 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2667 4576 4763 1700
Size: 110 857 1909 187 2869
Fragments arranged by size:
2869 1909 857 187 110
BsrGI T'GTAC_A
Cuts at: 96 3150 96
Size: 3054 2878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 3992 5004 3992
Size: 1012 4920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3058 794
Size: 2264 3668
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 2428 1184
Size: 1244 4688
BstYI r'GATC_y
Cuts at: 1972 2826 2925 3262 3629 4014 4031 5483 5569
Size: 854 99 337 367 385 17 1452 86
Cuts at: 5569 5581 5927 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 854 385 367 346 337 99
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2595 2040
Size: 555 5377
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5193
Fragments arranged by size:
5193 683 56
BtsI GCAGTG_nn'
Cuts at: 2352 3448 4242 4262 2352
Size: 1096 794 20 4022
Fragments arranged by size:
4022 1096 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 809
Size: 507 3623 1802
Fragments arranged by size:
3623 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897
Size: 54 1425 50 275 18 1549 889 1629
Cuts at: 5897 8
Size: 43
Fragments arranged by size:
1629 1549 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2352 3215 3862 1353
Size: 882 117 863 647 3423
Fragments arranged by size:
3423 882 863 647 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2014 2686 3021 4519 4893 5039
Size: 1064 138 425 672 335 1498 374 146
Cuts at: 5039 387
Size: 1280
Fragments arranged by size:
1498 1280 1064 672 425 374 335 146
138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 5932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2840 2849 2972 3083 3995 4592 5379
Size: 393 1208 9 123 111 912 597 787
Cuts at: 5379 5647 1239
Size: 268 1524
Fragments arranged by size:
1524 1208 912 787 597 393 268 123
111 9
EcoO109I rG'GnC_Cy
Cuts at: 2036 2932 3016 2036
Size: 896 84 4952
Fragments arranged by size:
4952 896 84
EcoRI G'AATT_C
Cuts at: 3241 3619 3241
Size: 378 5554
FspI TGC'GCA
Cuts at: 4438 4438
Size: 5932
HaeII r_GCGC'y
Cuts at: 1678 5079 1678
Size: 3401 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2816 2818 2834
Size: 32 135 32 779 32 433 2 16
Cuts at: 2834 2848 2850 2866 3029 3061 3077 3109 4573
Size: 14 2 16 163 32 16 32 1464
Cuts at: 4573 4605 4647 4679 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1464 779 433 163 135 42 32
32 32 32 32 32 32 16 16
16 14 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 2787 3276 3522 669
Size: 988 281 694 155 489 246 3079
Fragments arranged by size:
3079 988 694 489 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5932
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KpnI G_GTAC'C
Cuts at: 3257 3257
Size: 5932
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5046 5230 1305
Size: 3741 184 2007
Fragments arranged by size:
3741 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4110
Fragments arranged by size:
4110 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3890 4249 4408 518
Size: 648 2724 359 159 2042
Fragments arranged by size:
2724 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2656 2808 2839 2869 2917 3055 3348 4025
Size: 1050 152 31 30 48 138 293 677
Cuts at: 4025 5173 5418 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1050 677 293 245 152 138
48 31 30
NaeI GCC'GGC
Cuts at: 2669 2669
Size: 5932
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5380
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5932
NgoMIV G'CCGG_C
Cuts at: 2667 2667
Size: 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 3290 4835 1132
Size: 97 2061 1545 2229
Fragments arranged by size:
2229 2061 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5932
PciI A'CATG_T
Cuts at: 4831 4831
Size: 5932
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2935 1108
Size: 1827 4105
PfoI T'CCnGG_A
Cuts at: 2921 2928 2921
Size: 7 5925
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2932 3016 2036
Size: 896 84 4952
Fragments arranged by size:
4952 896 84
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5932
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PstI C_TGCA'G
Cuts at: 830 3126 3171 3284 830
Size: 2296 45 113 3478
Fragments arranged by size:
3478 2296 113 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
PvuII CAG'CTG
Cuts at: 2656 2869 2917 3055 2656
Size: 213 48 138 5533
Fragments arranged by size:
5533 213 138 48
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 5932
SalI G'TCGA_C
Cuts at: 1936 3274 1936
Size: 1338 4594
SapI GCTCTTCn'nnn_
Cuts at: 2235 3215 2235
Size: 980 4952
SbfI CC_TGCA'GG
Cuts at: 3284 3284
Size: 5932
ScaI AGT'ACT
Cuts at: 4180 5892 4180
Size: 1712 4220
SexAI A'CCwGG_T
Cuts at: 2934 2934
Size: 5932
SfcI C'TryA_G
Cuts at: 826 1045 3122 3167 3280 4415 5096 5287 826
Size: 219 2077 45 113 1135 681 191 1471
Fragments arranged by size:
2077 1471 1135 681 219 191 113 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5932
SmaI CCC'GGG
Cuts at: 1872 2910 3259 1872
Size: 1038 349 4545
Fragments arranged by size:
4545 1038 349
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2466 4036 4937 5199 820
Size: 197 926 523 1570 901 262 1553
Fragments arranged by size:
1570 1553 926 901 523 262 197
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 3290 1132
Size: 2158 3774
SspI AAT'ATT
Cuts at: 5 52 2200 3856 5
Size: 47 2148 1656 2081
Fragments arranged by size:
2148 2081 1656 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5932
StyI C'CwwG_G
Cuts at: 513 1065 2562 2732 3012 3371 513
Size: 552 1497 170 280 359 3074
Fragments arranged by size:
3074 1497 552 359 280 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 2791 3015 3959 3976 4129 4314
Size: 1255 761 450 224 944 17 153 185
Cuts at: 4314 4759 325
Size: 445 1498
Fragments arranged by size:
1498 1255 944 761 450 445 224 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3150 4178 5890
Size: 274 80 33 51 1443 1173 1028 1712
Cuts at: 5890 96
Size: 138
Fragments arranged by size:
1712 1443 1173 1028 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5892 5924 5892
Size: 32 5900
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5932
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XhoI C'TCGA_G
Cuts at: 1943 2466 1943
Size: 523 5409
XmaI C'CCGG_G
Cuts at: 1870 2908 3257 1870
Size: 1038 349 4545
Fragments arranged by size:
4545 1038 349
XmnI GAAnn'nnTTC
Cuts at: 4061 4061
Size: 5932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3740 276
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI XcmI