Restriction map for
pFN21AB8224
(Circular) (Six-base) MAPSORT of: pFN21AB8224.seq Check: 1761 from: 1 to: 5464
pFN21AB8224 5464 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2275 2275
Size: 5464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 2417 1166
Size: 1251 4213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2343 2374 2375 2342
Size: 1 31 1 5431
Fragments arranged by size:
5431 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2759 3207 4779 2759
Size: 448 1572 3444
Fragments arranged by size:
3444 1572 448
ApaI G_GGCC'C
Cuts at: 2565 2565
Size: 5464
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2709 2789 1870
Size: 73 262 504 80 4545
Fragments arranged by size:
4545 504 262 80 73
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 2785 4240 618
Size: 325 217 627 433 565 1455 1842
Fragments arranged by size:
1842 1455 627 565 433 325 217
BanII G_rGCy'C
Cuts at: 1391 2175 2565 2783 1391
Size: 784 390 218 4072
Fragments arranged by size:
4072 784 390 218
BbeI G_GCGC'C
Cuts at: 2224 2224
Size: 5464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2667 928
Size: 971 768 3725
Fragments arranged by size:
3725 971 768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2569 4075 136
Size: 107 122 71 631 605 897 1506 1525
Fragments arranged by size:
1525 1506 897 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2447 2565 3525 4681 1790
Size: 657 118 960 1156 2573
Fragments arranged by size:
2573 1156 960 657 118
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2559 3195 4153
Size: 416 53 83 399 24 1142 636 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 1142 958 636 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2349 2074
Size: 275 5189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2147 2444 2469 2610 4124 1239
Size: 896 12 297 25 141 1514 2579
Fragments arranged by size:
2579 1514 896 297 141 25 12
Bpu10I CC'TnA_GC
Cuts at: 2167 2167
Size: 5464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2221 2602 3271 3653
Size: 53 83 186 936 688 381 669 382
Cuts at: 3653 4288 275
Size: 635 1451
Fragments arranged by size:
1451 936 688 669 635 382 381 186
83 53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 1178
Size: 30 165 30 729 30 4480
Fragments arranged by size:
4480 729 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 4584
BseYI C'CCAG_C
Cuts at: 2545 2752 4667 5163 2545
Size: 207 1915 496 2846
Fragments arranged by size:
2846 1915 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2508 2663 1163
Size: 573 606 166 155 3964
Fragments arranged by size:
3964 606 573 166 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3525 3610 4681 3525
Size: 85 1071 4308
Fragments arranged by size:
4308 1071 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
BsmBI CGTCTCn'nnnn_
Cuts at: 2123 2123
Size: 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2447 2565 2783 3525 3610 4681
Size: 399 385 272 118 218 742 85 1071
Cuts at: 4681 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 385 272 218 118
85
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 2364 3353 5083 2364
Size: 989 1730 2745
Fragments arranged by size:
2745 1730 989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2275 2819 844
Size: 1431 544 3489
Fragments arranged by size:
3489 1431 544
BsrBI CCG'CTC
Cuts at: 1394 1823 2296 3351 1394
Size: 429 473 1055 3507
Fragments arranged by size:
3507 1055 473 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2473 3959 4133 65
Size: 1183 1225 1486 174 1396
Fragments arranged by size:
1486 1396 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2615 794
Size: 1821 3643
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2422 2011
Size: 411 5053
BstYI r'GATC_y
Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459
Size: 822 367 385 17 1452 86 12 346
Cuts at: 5459 1972
Size: 1977
Fragments arranged by size:
1977 1452 822 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2040 2591 513
Size: 552 939 27 9 551 3386
Fragments arranged by size:
3386 939 552 551 27 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3774 3794 2980
Size: 794 20 4650
Fragments arranged by size:
4650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2745 2911
Size: 54 1425 50 275 18 274 641 166
Cuts at: 2911 3800 5429 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 641 275 274 166 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2190 2570 3394 1353
Size: 831 6 380 824 3423
Fragments arranged by size:
3423 831 824 380 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2587 4051 4425 4571 387
Size: 1064 138 998 1464 374 146 1280
Fragments arranged by size:
1464 1280 1064 998 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2147 2444 2469 2610 3527 4124
Size: 393 503 12 297 25 141 917 597
Cuts at: 4124 4911 5179 1239
Size: 787 268 1524
Fragments arranged by size:
1524 917 787 597 503 393 297 268
141 25 12
EcoO109I rG'GnC_Cy
Cuts at: 2299 2561 2686 2299
Size: 262 125 5077
Fragments arranged by size:
5077 262 125
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2066 2224 2269 4611 1678
Size: 388 158 45 2342 2531
Fragments arranged by size:
2531 2342 388 158 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2486 2518 4105
Size: 32 135 32 560 32 322 32 1587
Cuts at: 4105 4137 4179 4211 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1587 560 322 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KasI G'GCGC_C
Cuts at: 2220 2220
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 2747 10
Size: 54 1425 343 274 641 2727
Fragments arranged by size:
2727 1425 641 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2417 3422 3781 3940 518
Size: 648 1203 48 1005 359 159 2042
Fragments arranged by size:
2042 1203 1005 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2227 2880 3557 4705 4950 1606
Size: 621 653 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 653 621 245
NarI GG'CG_CC
Cuts at: 2221 2221
Size: 5464
NcoI C'CATG_G
Cuts at: 513 1065 2004 2031 513
Size: 552 939 27 3946
Fragments arranged by size:
3946 939 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 2597 1108
Size: 1005 484 3975
Fragments arranged by size:
3975 1005 484
PfoI T'CCnGG_A
Cuts at: 2112 2175 2112
Size: 63 5401
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2299
Size: 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PspOMI G'GGCC_C
Cuts at: 2561 2561
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2346 2816 830
Size: 1516 470 3478
Fragments arranged by size:
3478 1516 470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2342 2812 3947 4628 4819 826
Size: 219 1297 470 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 470 219 191
SfoI GGC'GCC
Cuts at: 2222 2222
Size: 5464
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2207 2791 1872
Size: 335 584 4545
Fragments arranged by size:
4545 584 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2903 513
Size: 552 939 27 872 3074
Fragments arranged by size:
3074 939 872 552 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3508 3661 3846 4291 325
Size: 1255 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3710 5422 96
Size: 274 80 33 51 1443 1733 1712 138
Fragments arranged by size:
1733 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 2500 1316
Size: 1184 4280
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2205 2789 1870
Size: 335 584 4545
Fragments arranged by size:
4545 584 335
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI