Restriction map for
pFN21AB8223
(Circular) (Six-base) MAPSORT of: pFN21AB8223.seq Check: 8387 from: 1 to: 5521
pFN21AB8223 5521 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2440 2440
Size: 5521
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3584 4968 5236 1632
Size: 1952 1384 268 1917
Fragments arranged by size:
1952 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 2505 4420 5397 1203
Size: 1302 1915 977 1327
Fragments arranged by size:
1915 1327 1302 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 3264 4836 3264
Size: 1572 3949
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 1870
Size: 73 903 4545
Fragments arranged by size:
4545 903 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2842 4297 618
Size: 325 217 627 1055 1455 1842
Fragments arranged by size:
1842 1455 1055 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2493 2840 1391
Size: 1102 347 4072
Fragments arranged by size:
4072 1102 347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BbvCI CC'TCA_GC
Cuts at: 2646 2646
Size: 5521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2791 4132 136
Size: 107 122 71 631 605 1119 1341 1525
Fragments arranged by size:
1525 1341 1119 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2571 2613 2667 2938 2571
Size: 42 54 271 5154
Fragments arranged by size:
5154 271 54 42
Bme1580I G_kGCm'C
Cuts at: 1790 2106 3582 4738 1790
Size: 316 1476 1156 2573
Fragments arranged by size:
2573 1476 1156 316
BmgBI CAC'GTC
Cuts at: 1318 2506 1318
Size: 1188 4333
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2671 2458
Size: 213 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2428 2433 2792 4181 1239
Size: 1189 5 359 1389 2579
Fragments arranged by size:
2579 1389 1189 359 5
Bpu10I CC'TnA_GC
Cuts at: 2050 2646 2050
Size: 596 4925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3646 4511 4809 2369
Size: 1277 865 298 3081
Fragments arranged by size:
3081 1277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2446 4184 882
Size: 617 947 1738 2219
Fragments arranged by size:
2219 1738 947 617
BsaAI yAC'GTr
Cuts at: 493 1204 2225 3312 493
Size: 711 1021 1087 2702
Fragments arranged by size:
2702 1087 1021 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3328 3710 4345 275
Size: 53 83 186 936 1795 382 635 1451
Fragments arranged by size:
1795 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2055 2085 1178
Size: 30 165 30 652 30 4614
Fragments arranged by size:
4614 652 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2795 1186
Size: 1609 3912
BseYI C'CCAG_C
Cuts at: 2099 4724 5220 2099
Size: 2625 496 2400
Fragments arranged by size:
2625 2400 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 1163
Size: 573 423 4525
Fragments arranged by size:
4525 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2971 3732 4760 1540
Size: 275 186 970 761 1028 2301
Fragments arranged by size:
2301 1028 970 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2805 3582 3667 4738 2805
Size: 777 85 1071 3588
Fragments arranged by size:
3588 1071 777 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2493 2805 2840 3582 3667 4738
Size: 399 316 387 312 35 742 85 1071
Cuts at: 4738 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 387 316 312 85
35
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 2790 2876 844
Size: 1596 350 86 3489
Fragments arranged by size:
3489 1596 350 86
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4165 4352 1700
Size: 110 2355 187 2869
Fragments arranged by size:
2869 2355 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2800 794
Size: 2006 3515
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 2599 2686 2566
Size: 33 87 5401
Fragments arranged by size:
5401 87 33
BstYI r'GATC_y
Cuts at: 1972 2379 2851 3218 3603 3620 5072 5158 5170
Size: 407 472 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 472 407 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2131 2180 513
Size: 552 939 127 49 3854
Fragments arranged by size:
3854 939 552 127 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2728 487
Size: 683 56 1502 3280
Fragments arranged by size:
3280 1502 683 56
BtsI GCAGTG_nn'
Cuts at: 2276 2691 3037 3831 3851 2276
Size: 415 346 794 20 3946
Fragments arranged by size:
3946 794 415 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2631 2688 2968
Size: 54 1425 50 275 18 801 57 280
Cuts at: 2968 3857 5486 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 801 280 275 57 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 1537
Size: 275 1156 4090
Fragments arranged by size:
4090 1156 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 3451 1353
Size: 941 1157 3423
Fragments arranged by size:
3423 1157 941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2428 2433 2792 3584 4181 4968 5236
Size: 393 796 5 359 792 597 787 268
Cuts at: 5236 1239
Size: 1524
Fragments arranged by size:
1524 796 792 787 597 393 359 268
5
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 2282 2720 4027 2282
Size: 438 1307 3776
Fragments arranged by size:
3776 1307 438
HaeII r_GCGC'y
Cuts at: 1678 4668 1678
Size: 2990 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 2406 2424 2438
Size: 32 135 32 528 32 274 18 14
Cuts at: 2438 2456 2492 2524 4162 4194 4236 4268 1373
Size: 18 36 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 528 274 135 42 36 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2865 3111 669
Size: 988 281 331 596 246 3079
Fragments arranged by size:
3079 988 596 331 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 10
Size: 54 1425 343 858 2841
Fragments arranged by size:
2841 1425 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 3838 3997 518
Size: 648 2313 359 159 2042
Fragments arranged by size:
2313 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2666 2789 2812 2937 3614 4762 5007 1606
Size: 1060 123 23 125 677 1148 245 2120
Fragments arranged by size:
2120 1148 1060 677 245 125 123 23
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PfoI T'CCnGG_A
Cuts at: 2496 2496
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 5489
PstI C_TGCA'G
Cuts at: 830 2698 2737 2873 830
Size: 1868 39 136 3478
Fragments arranged by size:
3478 1868 136 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
PvuII CAG'CTG
Cuts at: 2666 2812 2666
Size: 146 5375
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2694 2733 2869 4004 4685 4876 826
Size: 219 1649 39 136 1135 681 191 1471
Fragments arranged by size:
1649 1471 1135 681 219 191 136 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2848 1872
Size: 976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3625 4526 4788 820
Size: 197 926 441 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 441 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2658 2960 513
Size: 552 939 654 302 3074
Fragments arranged by size:
3074 939 654 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 2441 3548 3565 3718 3903 4348
Size: 1255 559 302 1107 17 153 185 445
Cuts at: 4348 325
Size: 1498
Fragments arranged by size:
1498 1255 1107 559 445 302 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 2268 2300 5481 5513 2055
Size: 32 181 32 3181 32 2063
Fragments arranged by size:
3181 2063 181 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2599
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2846 1870
Size: 976 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 3650 2537
Size: 1113 4408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI