Restriction map for
pFN21AB8221
(Circular) (Six-base) MAPSORT of: pFN21AB8221.seq Check: 2136 from: 1 to: 5449
pFN21AB8221 5449 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 2617 4178 2617
Size: 1561 3888
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 2324 2630 3192 4764 2324
Size: 306 562 1572 3009
Fragments arranged by size:
3009 1572 562 306
ApaI G_GGCC'C
Cuts at: 2190 2190
Size: 5449
ApaLI G'TGCA_C
Cuts at: 2744 3506 4662 2744
Size: 762 1156 3531
Fragments arranged by size:
3531 1156 762
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 2774 1870
Size: 73 240 591 4545
Fragments arranged by size:
4545 591 240 73
BamHI G'GATC_C
Cuts at: 2017 2779 3146 2017
Size: 762 367 4320
Fragments arranged by size:
4320 762 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2039 2078 2177 2770
Size: 325 217 627 240 12 39 99 593
Cuts at: 2770 4225 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 593 325 240 217 99
39 12
BanII G_rGCy'C
Cuts at: 1391 2190 2768 1391
Size: 799 578 4072
Fragments arranged by size:
4072 799 578
BbeI G_GCGC'C
Cuts at: 2031 2043 2082 2181 2031
Size: 12 39 99 5299
Fragments arranged by size:
5299 99 39 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 928
Size: 971 198 4280
Fragments arranged by size:
4280 971 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2342 3341 4551 2342
Size: 999 1210 3240
Fragments arranged by size:
3240 1210 999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 4060 136
Size: 107 122 71 631 605 439 1949 1525
Fragments arranged by size:
1949 1525 631 605 439 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2010 2866 2010
Size: 856 4593
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2748 3510 4666 1790
Size: 400 558 762 1156 2573
Fragments arranged by size:
2573 1156 762 558 400
BmgBI CAC'GTC
Cuts at: 1318 2530 1318
Size: 1212 4237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4109 1239
Size: 2870 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 3574 4439 4737 2711
Size: 863 865 298 3423
Fragments arranged by size:
3423 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2040 2079 2178
Size: 53 83 186 936 495 12 39 99
Cuts at: 2178 3256 3638 4273 275
Size: 1078 382 635 1451
Fragments arranged by size:
1451 1078 936 635 495 382 186 99
83 53 39 12
BsaWI w'CCGG_w
Cuts at: 2218 3882 4280 4554 4701 5112 2218
Size: 1664 398 274 147 411 2555
Fragments arranged by size:
2555 1664 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5224
Fragments arranged by size:
5224 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5449
BseYI C'CCAG_C
Cuts at: 2059 2190 4652 5148 2059
Size: 131 2462 496 2360
Fragments arranged by size:
2462 2360 496 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4876
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2748 3510 3595 4666 2748
Size: 762 85 1071 3531
Fragments arranged by size:
3531 1071 762 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2748 2768 3510 3595 4666 1391
Size: 399 400 558 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 558 400 399 85 20
BspEI T'CCGG_A
Cuts at: 2218 5112 2218
Size: 2894 2555
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2075 2081 4093 4280 1700
Size: 110 226 39 6 2012 187 2869
Fragments arranged by size:
2869 2012 226 187 110 39 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2017 2779 3146 3531 3548 5000 5086 5098
Size: 45 762 367 385 17 1452 86 12
Cuts at: 5098 5444 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 762 385 367 346 86 45
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3958
Fragments arranged by size:
3958 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3759 3779 2965
Size: 794 20 4635
Fragments arranged by size:
4635 794 20
ClaI AT'CG_AT
Cuts at: 1554 2308 1554
Size: 754 4695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DraIII CAC_nnn'GTG
Cuts at: 2634 2634
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2896 3785
Size: 54 1425 50 275 18 243 823 889
Cuts at: 3785 5414 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 823 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3379 1353
Size: 2026 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3512 4109 4896 5164 1239
Size: 393 1880 597 787 268 1524
Fragments arranged by size:
1880 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 2729 2576
Size: 153 5296
EcoO109I rG'GnC_Cy
Cuts at: 2187 2734 2187
Size: 547 4902
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2031 2043 2082 2181 4596 1678
Size: 353 12 39 99 2415 2531
Fragments arranged by size:
2531 2415 353 99 39 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 4090 4122 4164
Size: 32 135 32 1031 32 1455 32 42
Cuts at: 4164 4196 1373
Size: 32 2626
Fragments arranged by size:
2626 1455 1031 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 2502 748
Size: 1754 3695
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KasI G'GCGC_C
Cuts at: 2027 2039 2078 2177 2027
Size: 12 39 99 5299
Fragments arranged by size:
5299 99 39 12
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2297 4563 4747 1305
Size: 992 2266 184 2007
Fragments arranged by size:
2266 2007 992 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3627
Fragments arranged by size:
3627 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2740 2865 3542 4690 4935 1606
Size: 1134 125 677 1148 245 2120
Fragments arranged by size:
2120 1148 1134 677 245 125
NaeI GCC'GGC
Cuts at: 2038 2077 2083 2038
Size: 39 6 5404
Fragments arranged by size:
5404 39 6
NarI GG'CG_CC
Cuts at: 2028 2040 2079 2178 2028
Size: 12 39 99 5299
Fragments arranged by size:
5299 99 39 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3958
Fragments arranged by size:
3958 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NgoMIV G'CCGG_C
Cuts at: 2036 2075 2081 2036
Size: 39 6 5404
Fragments arranged by size:
5404 39 6
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2707 2807 4352 1132
Size: 97 1478 100 1545 2229
Fragments arranged by size:
2229 1545 1478 100 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PfoI T'CCnGG_A
Cuts at: 2166 2166
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2186 2186
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2801 830
Size: 1971 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2465 2566 2797 3932 4613 4804 826
Size: 219 1420 101 231 1135 681 191 1471
Fragments arranged by size:
1471 1420 1135 681 231 219 191 101
SfoI GGC'GCC
Cuts at: 2029 2041 2080 2179 2029
Size: 12 39 99 5299
Fragments arranged by size:
5299 99 39 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 2081 1700
Size: 381 5068
SmaI CCC'GGG
Cuts at: 1872 2185 2776 1872
Size: 313 591 4545
Fragments arranged by size:
4545 591 313
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3553 4454 4716 820
Size: 197 926 783 827 901 262 1553
Fragments arranged by size:
1553 926 901 827 783 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2707 2807 1132
Size: 1575 100 3774
Fragments arranged by size:
3774 1575 100
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 2156 1517
Size: 639 4810
StyI C'CwwG_G
Cuts at: 513 1065 2004 2888 513
Size: 552 939 884 3074
Fragments arranged by size:
3074 939 884 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3695 5407 96
Size: 274 80 33 51 1443 1718 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2264 2631 2011
Size: 253 367 4829
Fragments arranged by size:
4829 367 253
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2183 2774 1870
Size: 313 591 4545
Fragments arranged by size:
4545 591 313
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI