Restriction map for
pFN21AB8220
(Circular) (Six-base) MAPSORT of: pFN21AB8220.seq Check: 6356 from: 1 to: 6001
pFN21AB8220 6001 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2349 3811 278
Size: 53 83 186 936 813 1462 2468
Fragments arranged by size:
2468 1462 936 813 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2349 3344 1937
Size: 412 995 4594
Fragments arranged by size:
4594 995 412
Acc65I G'GTAC_C
Cuts at: 2772 3322 2772
Size: 550 5451
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 2660 2893 3193 4730 2660
Size: 233 300 1537 3931
Fragments arranged by size:
3931 1537 300 233
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2582 2614 2582
Size: 32 5969
AlwNI CAG_nnn'CTG
Cuts at: 2446 2804 3058 3744 5316 2446
Size: 358 254 686 1572 3131
Fragments arranged by size:
3131 1572 686 358 254
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2199 3326 1870
Size: 73 205 51 1127 4545
Fragments arranged by size:
4545 1127 205 73 51
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2772 2862 3322 4777
Size: 325 217 627 318 667 90 460 1455
Cuts at: 4777 618
Size: 1842
Fragments arranged by size:
1842 1455 667 627 460 325 318 217
90
BanII G_rGCy'C
Cuts at: 1391 2232 2331 3320 1391
Size: 841 99 989 4072
Fragments arranged by size:
4072 989 841 99
BbeI G_GCGC'C
Cuts at: 2866 2866
Size: 6001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3242 928
Size: 971 1343 3687
Fragments arranged by size:
3687 1343 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BfrBI ATG'CAT
Cuts at: 2755 2755
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 4612 136
Size: 107 122 71 631 605 874 2066 1525
Fragments arranged by size:
2066 1525 874 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 2301 2358 3418 2301
Size: 57 1060 4884
Fragments arranged by size:
4884 1060 57
Bme1580I G_kGCm'C
Cuts at: 1790 2176 4062 5218 1790
Size: 386 1886 1156 2573
Fragments arranged by size:
2573 1886 1156 386
BmgBI CAC'GTC
Cuts at: 1318 2680 1318
Size: 1362 4639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 4690 442
Size: 416 53 83 399 24 2315 958 1753
Fragments arranged by size:
2315 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 2453 2582 2603 2621 2912 2942 3017
Size: 1196 18 129 21 18 291 30 75
Cuts at: 3017 4661 1239
Size: 1644 2579
Fragments arranged by size:
2579 1644 1196 291 129 75 30 21
18 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2623 3002 3200 4126 4991 5289 2623
Size: 379 198 926 865 298 3335
Fragments arranged by size:
3335 926 865 379 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2189 4664 882
Size: 617 690 2475 2219
Fragments arranged by size:
2475 2219 690 617
BsaAI yAC'GTr
Cuts at: 493 1204 2956 3792 493
Size: 711 1752 836 2702
Fragments arranged by size:
2702 1752 836 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 2741 2863 3808
Size: 53 83 186 936 813 395 122 945
Cuts at: 3808 4190 4825 275
Size: 382 635 1451
Fragments arranged by size:
1451 945 936 813 635 395 382 186
122 83 53
BsaWI w'CCGG_w
Cuts at: 2595 3129 4434 4832 5106 5253 5664 2595
Size: 534 1305 398 274 147 411 2932
Fragments arranged by size:
2932 1305 534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2582 2612 1178
Size: 30 165 30 1179 30 4567
Fragments arranged by size:
4567 1179 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2041 2600 2603 2633 2636 2960 3137
Size: 840 15 559 3 30 3 324 177
Cuts at: 3137 1186
Size: 4050
Fragments arranged by size:
4050 840 559 324 177 30 15 3
3
BseYI C'CCAG_C
Cuts at: 2168 5204 5700 2168
Size: 3036 496 2469
Fragments arranged by size:
3036 2469 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3167 1163
Size: 573 1431 3997
Fragments arranged by size:
3997 1431 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 3288 4062 4147 5218 2232
Size: 1056 774 85 1071 3015
Fragments arranged by size:
3015 1071 1056 774 85
BsmI GAATG_Cn'
Cuts at: 2133 3512 3605 2133
Size: 1379 93 4529
Fragments arranged by size:
4529 1379 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2008 2053 2008
Size: 45 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2232 2331 3288 3320 4062 4147
Size: 399 386 56 99 957 32 742 85
Cuts at: 4147 5218 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 957 742 399 386 99 85
56 32
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2650 2891 3066 3171 3356 844
Size: 1806 241 175 105 185 3489
Fragments arranged by size:
3489 1806 241 185 175 105
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 2212 2778 2950 3888 1394
Size: 429 217 172 566 172 938 3507
Fragments arranged by size:
3507 938 566 429 217 172 172
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 4496 4670 65
Size: 1183 1494 1754 174 1396
Fragments arranged by size:
1754 1494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2859 4645 4832 1700
Size: 110 1049 1786 187 2869
Fragments arranged by size:
2869 1786 1049 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 2615 4061 5073 2615
Size: 1446 1012 3543
Fragments arranged by size:
3543 1446 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2180 794
Size: 1386 4615
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2338
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 2557 2592 2610 2715 3331 3698 4083 4100
Size: 585 35 18 105 616 367 385 17
Cuts at: 4100 5552 5638 5650 5996 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 616 585 385 367 346 105
86 35 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2053 2486 2053
Size: 433 5568
BtgI C'CryG_G
Cuts at: 513 1065 2004 3240 513
Size: 552 939 1236 3274
Fragments arranged by size:
3274 1236 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 3517 4311 4331 3517
Size: 794 20 5187
Fragments arranged by size:
5187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2448 2682
Size: 54 1425 50 275 18 256 362 234
Cuts at: 2682 2967 3448 4337 5966 8
Size: 285 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 362 285 275 256
234 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 2997 3931 1353
Size: 1407 237 934 3423
Fragments arranged by size:
3423 1407 934 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2819 4588 4962 5108 387
Size: 1064 138 1230 1769 374 146 1280
Fragments arranged by size:
1769 1280 1230 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2230 2230
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2435 2453 2582 2603 2621 2912 2942
Size: 393 803 18 129 21 18 291 30
Cuts at: 2942 3017 4064 4661 5448 5716 1239
Size: 75 1047 597 787 268 1524
Fragments arranged by size:
1524 1047 803 787 597 393 291 268
129 75 30 21 18 18
EcoO109I rG'GnC_Cy
Cuts at: 2074 2099 2443 3055 2074
Size: 25 344 612 5020
Fragments arranged by size:
5020 612 344 25
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 6001
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2294 2409 2866 2987 5148 1678
Size: 616 115 457 121 2161 2531
Fragments arranged by size:
2531 2161 616 457 121 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2416 2448 2646 2678 2879
Size: 32 135 32 844 32 198 32 201
Cuts at: 2879 2911 3179 3211 4642 4674 4716 4748 1373
Size: 32 268 32 1431 32 42 32 2626
Fragments arranged by size:
2626 1431 844 268 201 198 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2862 2862
Size: 6001
KpnI G_GTAC'C
Cuts at: 2776 3326 2776
Size: 550 5451
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3183 5115 5299 1305
Size: 1878 1932 184 2007
Fragments arranged by size:
2007 1932 1878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2450 2684 10
Size: 54 1425 343 618 234 3327
Fragments arranged by size:
3327 1425 618 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3143 3230 3291 3959 4318 4477 518
Size: 648 1977 87 61 668 359 159 2042
Fragments arranged by size:
2042 1977 668 648 359 159 87 61
MspA1I CmG'CkG
Cuts at: 1606 2030 2314 2647 3417 4094 5242 5487 1606
Size: 424 284 333 770 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 677 424 333 284 245
NarI GG'CG_CC
Cuts at: 2863 2863
Size: 6001
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4510
Fragments arranged by size:
4510 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NsiI A_TGCA'T
Cuts at: 2757 2757
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2755 3359 4904 1132
Size: 97 1526 604 1545 2229
Fragments arranged by size:
2229 1545 1526 604 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6001
PfoI T'CCnGG_A
Cuts at: 2271 2469 2637 2271
Size: 198 168 5635
Fragments arranged by size:
5635 198 168
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PmlI CAC'GTG
Cuts at: 2956 2956
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2614 4070 4102 5583 5615 2582
Size: 32 1456 32 1481 32 2968
Fragments arranged by size:
2968 1481 1456 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 2443 3055 2099
Size: 344 612 5045
Fragments arranged by size:
5045 612 344
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 3168 3353 830
Size: 2338 185 3478
Fragments arranged by size:
3478 2338 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2314 2647 2314
Size: 333 5668
SacI G_AGCT'C
Cuts at: 2232 2232
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SfcI C'TryA_G
Cuts at: 826 1045 2518 3100 3164 3349 4484 5165 5356
Size: 219 1473 582 64 185 1135 681 191
Cuts at: 5356 826
Size: 1471
Fragments arranged by size:
1473 1471 1135 681 582 219 191 185
64
SfoI GGC'GCC
Cuts at: 2864 2864
Size: 6001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 2150 2201 3328 1872
Size: 278 51 1127 4545
Fragments arranged by size:
4545 1127 278 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2602 3017 3215 4105 5006 5268
Size: 197 926 659 415 198 890 901 262
Cuts at: 5268 820
Size: 1553
Fragments arranged by size:
1553 926 901 890 659 415 262 198
197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 3440 513
Size: 552 939 318 1118 3074
Fragments arranged by size:
3074 1118 939 552 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4028 4045 4198 4383 4828 325
Size: 1255 2448 17 153 185 445 1498
Fragments arranged by size:
2448 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4247 5959 96
Size: 274 80 33 51 1443 2270 1712 138
Fragments arranged by size:
2270 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 2488
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 2148 2199 3326 1870
Size: 278 51 1127 4545
Fragments arranged by size:
4545 1127 278 51
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2347 3809 276
Size: 53 83 186 936 813 1462 2468
Fragments arranged by size:
2468 1462 936 813 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI