Restriction map for
pFN21AB8219
(Circular) (Six-base) MAPSORT of: pFN21AB8219.seq Check: 1913 from: 1 to: 5656
pFN21AB8219 5656 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2566 2566
Size: 5656
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 3719 5103 5371 1632
Size: 996 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 996 268
AflII C'TTAA_G
Cuts at: 820 1017 2048 820
Size: 197 1031 4428
Fragments arranged by size:
4428 1031 197
AflIII A'CryG_T
Cuts at: 1203 2304 4555 5532 1203
Size: 1101 2251 977 1327
Fragments arranged by size:
2251 1327 1101 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2333 2679 3399 4971 2333
Size: 346 720 1572 3018
Fragments arranged by size:
3018 1572 720 346
ApaI G_GGCC'C
Cuts at: 2400 2400
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 2399 2981 1870
Size: 73 450 6 582 4545
Fragments arranged by size:
4545 582 450 73 6
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 2323 2345 2453 2977
Size: 325 217 627 418 118 22 108 524
Cuts at: 2977 4432 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 524 418 325 217 118
108 22
BanII G_rGCy'C
Cuts at: 1391 2114 2400 2975 1391
Size: 723 286 575 4072
Fragments arranged by size:
4072 723 575 286
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 2411 928
Size: 971 123 389 4173
Fragments arranged by size:
4173 971 389 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3847 3881 1903
Size: 34 50 34 1826 34 3678
Fragments arranged by size:
3678 1826 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 2484 1548
Size: 936 4720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2810 2831 2846
Size: 107 122 71 631 605 1138 21 15
Cuts at: 2846 4267 136
Size: 1421 1525
Fragments arranged by size:
1525 1421 1138 631 605 122 107 71
21 15
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2400 2456 3717 4873 1790
Size: 418 192 56 1261 1156 2573
Fragments arranged by size:
2573 1261 1156 418 192 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2431 2514 2672 4316 1239
Size: 1010 182 83 158 1644 2579
Fragments arranged by size:
2579 1644 1010 182 158 83
Bpu10I CC'TnA_GC
Cuts at: 2892 2892
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 2951 4319 882
Size: 617 897 555 1368 2219
Fragments arranged by size:
2219 1368 897 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 2073 5650 2073
Size: 3577 2079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2487 2517 2851 2881 1178
Size: 30 165 30 1084 30 334 30 3953
Fragments arranged by size:
3953 1084 334 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2933 1186
Size: 1747 3909
BseYI C'CCAG_C
Cuts at: 2221 2887 4859 5355 2221
Size: 666 1972 496 2522
Fragments arranged by size:
2522 1972 666 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 1163
Size: 573 762 4321
Fragments arranged by size:
4321 762 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 2638 3167 3260 2638
Size: 529 93 5034
Fragments arranged by size:
5034 529 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2759 2759
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2208 2400 2456 2975 3717 3802
Size: 399 324 94 192 56 519 742 85
Cuts at: 3802 4873 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 519 399 324 192 94
85 56
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2566 3011 844
Size: 1722 445 3489
Fragments arranged by size:
3489 1722 445
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2349 2671 4151 4325 65
Size: 1183 1101 322 1480 174 1396
Fragments arranged by size:
1480 1396 1183 1101 322 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 2387
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2130 2986 3353 3738 3755 5207 5293 5305
Size: 158 856 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 856 385 367 346 158 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3966 3986 3172
Size: 794 20 4842
Fragments arranged by size:
4842 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2032 4663 809
Size: 507 716 2631 1802
Fragments arranged by size:
2631 1802 716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 3103 3992
Size: 54 1425 50 275 18 483 790 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 790 483 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2904 3586 1353
Size: 1551 682 3423
Fragments arranged by size:
3423 1551 682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2431 2514 2628 2672 3719 4316
Size: 393 617 182 83 114 44 1047 597
Cuts at: 4316 5103 5371 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 787 617 597 393 268 182
114 83 44
EcoO109I rG'GnC_Cy
Cuts at: 2284 2284
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
EcoRV GAT'ATC
Cuts at: 2620 2620
Size: 5656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2824 2428
Size: 396 5260
FspI TGC'GCA
Cuts at: 2189 4162 2189
Size: 1973 3683
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2931 2963 4297 4329 4371
Size: 32 135 32 1359 32 1334 32 42
Cuts at: 4371 4403 1373
Size: 32 2626
Fragments arranged by size:
2626 1359 1334 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2083 2895 2904 4770 4954 1305
Size: 756 22 812 9 1866 184 2007
Fragments arranged by size:
2007 1866 812 756 184 22 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 10
Size: 54 1425 343 483 3351
Fragments arranged by size:
3351 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3614 3973 4132 518
Size: 648 1017 1431 359 159 2042
Fragments arranged by size:
2042 1431 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 2482 2864 3072 3749 4897 5142 1606
Size: 724 152 382 208 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 382 245 208 152
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2140 2308 2536 3014 4559 1132
Size: 97 911 168 228 478 1545 2229
Fragments arranged by size:
2229 1545 911 478 228 168 97
PasI CC'CwG_GG
Cuts at: 1348 2010 1348
Size: 662 4994
PciI A'CATG_T
Cuts at: 2304 4555 2304
Size: 2251 3405
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 1108
Size: 957 4699
PfoI T'CCnGG_A
Cuts at: 2649 2649
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2284
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2396 2396
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2688 2884 3008 830
Size: 1858 196 124 3478
Fragments arranged by size:
3478 1858 196 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2330 2482 2864 2330
Size: 152 382 5122
Fragments arranged by size:
5122 382 152
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2684 2880 3004 4139 4820 5011 826
Size: 219 1639 196 124 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 681 219 196 191 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 3760 4661 4923 820
Size: 197 926 105 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197 105
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2804 1517
Size: 1287 4369
StyI C'CwwG_G
Cuts at: 513 1065 2277 2904 3095 513
Size: 552 1212 627 191 3074
Fragments arranged by size:
3074 1212 627 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2487 2519 2851 2883 5616 5648 2487
Size: 32 332 32 2733 32 2495
Fragments arranged by size:
2733 2495 332 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 3785 2084
Size: 1701 3955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI