Restriction map for
pFN21AB8217
(Circular) (Six-base) MAPSORT of: pFN21AB8217.seq Check: 8490 from: 1 to: 5584
pFN21AB8217 5584 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 5584
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2853 2927 1937
Size: 916 74 4594
Fragments arranged by size:
4594 916 74
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3647 5031 5299 1632
Size: 2015 1384 268 1917
Fragments arranged by size:
2015 1917 1384 268
AfeI AGC'GCT
Cuts at: 2168 2168
Size: 5584
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 2460 4483 5460 1203
Size: 1257 2023 977 1327
Fragments arranged by size:
2023 1327 1257 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 2568 4313 2568
Size: 1745 3839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AlwNI CAG_nnn'CTG
Cuts at: 2662 2838 3327 4899 2662
Size: 176 489 1572 3347
Fragments arranged by size:
3347 1572 489 176
ApaI G_GGCC'C
Cuts at: 2026 2198 2803 2026
Size: 172 605 4807
Fragments arranged by size:
4807 605 172
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2893 3119 3271 5380 1493
Size: 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 2769 2909 1870
Size: 73 343 483 140 4545
Fragments arranged by size:
4545 483 343 140 73
BamHI G'GATC_C
Cuts at: 2914 3281 2914
Size: 367 5217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2107 2149 2597 2905 4360
Size: 325 217 627 320 42 448 308 1455
Cuts at: 4360 618
Size: 1842
Fragments arranged by size:
1842 1455 627 448 325 320 308 217
42
BanII G_rGCy'C
Cuts at: 1391 2026 2198 2547 2631 2742 2803 2903 1391
Size: 635 172 349 84 111 61 100 4072
Fragments arranged by size:
4072 635 349 172 111 100 84 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3775 3809 1903
Size: 34 50 34 1754 34 3678
Fragments arranged by size:
3678 1754 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2229 4195 136
Size: 107 122 71 631 605 557 1966 1525
Fragments arranged by size:
1966 1525 631 605 557 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 2865 3001 2865
Size: 136 5448
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2198 2803 3645 4801 1790
Size: 236 172 605 842 1156 2573
Fragments arranged by size:
2573 1156 842 605 236 172
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2020 2255 2736
Size: 416 53 83 399 24 603 235 481
Cuts at: 2736 3315 4273 442
Size: 579 958 1753
Fragments arranged by size:
1753 958 603 579 481 416 399 235
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2485 2561 2579 2741 2756 4244 1239
Size: 1246 76 18 162 15 1488 2579
Fragments arranged by size:
2579 1488 1246 162 76 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2203 2383 2600 3709 4574 4872 2203
Size: 180 217 1109 865 298 2915
Fragments arranged by size:
2915 1109 865 298 217 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 2555 4247 882
Size: 617 852 204 1692 2219
Fragments arranged by size:
2219 1692 852 617 204
BsaAI yAC'GTr
Cuts at: 493 1204 2461 3375 493
Size: 711 1257 914 2702
Fragments arranged by size:
2702 1257 914 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2778 3391 3773 4408
Size: 53 83 186 936 1245 613 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1245 936 635 613 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2589 4017 4415 4689 4836 5247 2589
Size: 1428 398 274 147 411 2926
Fragments arranged by size:
2926 1428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5584
BseYI C'CCAG_C
Cuts at: 4787 5283 4787
Size: 496 5088
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5011
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3795 4823 1540
Size: 275 186 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2539 2631 3645 3730 4801 2539
Size: 92 1014 85 1071 3322
Fragments arranged by size:
3322 1071 1014 92 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2198 2539 2547 2631 2742 2803
Size: 399 236 172 341 8 84 111 61
Cuts at: 2803 2903 3645 3730 4801 1391
Size: 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 236 172 111
100 85 84 61 8
BspEI T'CCGG_A
Cuts at: 2589 5247 2589
Size: 2658 2926
BspHI T'CATG_A
Cuts at: 2439 3473 5203 2439
Size: 1034 1730 2820
Fragments arranged by size:
2820 1730 1034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 2939 844
Size: 1275 820 3489
Fragments arranged by size:
3489 1275 820
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2269 2409 2749 4228 4415 1700
Size: 110 459 140 340 1479 187 2869
Fragments arranged by size:
2869 1479 459 340 187 140 110
BsrGI T'GTAC_A
Cuts at: 96 2463 96
Size: 2367 3217
BssHII G'CGCG_C
Cuts at: 1925 2012 1925
Size: 87 5497
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579
Size: 942 367 385 17 1452 86 12 346
Cuts at: 5579 1972
Size: 1977
Fragments arranged by size:
1977 1452 942 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5032
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3100 3894 3914 2641
Size: 459 794 20 4311
Fragments arranged by size:
4311 794 459 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2407 2664 2782
Size: 54 1425 50 275 18 577 257 118
Cuts at: 2782 3031 3920 5549 8
Size: 249 889 1629 43
Fragments arranged by size:
1629 1425 889 577 275 257 249 118
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3514 1353
Size: 2161 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 5584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2485 2561 2579 2741 2756 3647 4244
Size: 393 853 76 18 162 15 891 597
Cuts at: 4244 5031 5299 1239
Size: 787 268 1524
Fragments arranged by size:
1524 891 853 787 597 393 268 162
76 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2699 2699
Size: 5584
EcoO109I rG'GnC_Cy
Cuts at: 2022 2305 2451 2659 2692 2022
Size: 283 146 208 33 4914
Fragments arranged by size:
4914 283 208 146 33
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 4090 4090
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 2170 4731 1678
Size: 492 2561 2531
Fragments arranged by size:
2561 2531 492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2334 2366 2554 2586 4225
Size: 32 135 32 762 32 188 32 1639
Cuts at: 4225 4257 4299 4331 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1639 762 188 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2854 2928 3174 669
Size: 988 281 916 74 246 3079
Fragments arranged by size:
3079 988 916 281 246 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2232 4698 4882 1305
Size: 813 114 2466 184 2007
Fragments arranged by size:
2466 2007 813 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 10
Size: 54 1425 343 834 2928
Fragments arranged by size:
2928 1425 834 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 3542 3901 4060 518
Size: 648 1272 1104 359 159 2042
Fragments arranged by size:
2042 1272 1104 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 3000 3677 4825 5070 1606
Size: 413 981 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 677 413 245
NaeI GCC'GGC
Cuts at: 2271 2411 2751 2271
Size: 140 340 5104
Fragments arranged by size:
5104 340 140
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5032
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NgoMIV G'CCGG_C
Cuts at: 2269 2409 2749 2269
Size: 140 340 5104
Fragments arranged by size:
5104 340 140
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2437 2482 2942 4487 1132
Size: 97 1208 45 460 1545 2229
Fragments arranged by size:
2229 1545 1208 460 97 45
PasI CC'CwG_GG
Cuts at: 1348 2160 2838 1348
Size: 812 678 4094
Fragments arranged by size:
4094 812 678
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2662 1108
Size: 1554 4030
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 5166 5198 3653
Size: 32 1481 32 4039
Fragments arranged by size:
4039 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2451 2692 2305
Size: 146 241 5197
Fragments arranged by size:
5197 241 146
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PspOMI G'GGCC_C
Cuts at: 2022 2194 2799 2022
Size: 172 605 4807
Fragments arranged by size:
4807 605 172
PstI C_TGCA'G
Cuts at: 830 2116 2636 2936 830
Size: 1286 520 300 3478
Fragments arranged by size:
3478 1286 520 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2852 2926 1936
Size: 916 74 4594
Fragments arranged by size:
4594 916 74
SanDI GG'GwC_CC
Cuts at: 2451 2451
Size: 5584
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2112 2632 2754 2932 4067 4748 4939
Size: 219 1067 520 122 178 1135 681 191
Cuts at: 4939 826
Size: 1471
Fragments arranged by size:
1471 1135 1067 681 520 219 191 178
122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2288 2771 2911 1872
Size: 416 483 140 4545
Fragments arranged by size:
4545 483 416 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2218 2398 2615 3688 4589 4851
Size: 197 926 275 180 217 1073 901 262
Cuts at: 4851 820
Size: 1553
Fragments arranged by size:
1553 1073 926 901 275 262 217 197
180
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SrfI GCCC'GGGC
Cuts at: 2288 2288
Size: 5584
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 3023 513
Size: 552 1958 3074
Fragments arranged by size:
3074 1958 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2375 3611 3628 3781 3966 4411 325
Size: 1255 795 1236 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1236 795 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2463 2481 3830
Size: 274 80 33 51 1443 486 18 1349
Cuts at: 3830 5542 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1349 486 274 138 80 51
33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 2117 2149 5544 5576 2075
Size: 32 10 32 3395 32 2083
Fragments arranged by size:
3395 2083 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2402
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2286 2769 2909 1870
Size: 416 483 140 4545
Fragments arranged by size:
4545 483 416 140
XmnI GAAnn'nnTTC
Cuts at: 3713 3713
Size: 5584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SwaI