Restriction map for
pFN21AB8216
(Circular) (Six-base) MAPSORT of: pFN21AB8216.seq Check: 1348 from: 1 to: 5350
pFN21AB8216 5350 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3160 278
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2337 2693 1937
Size: 400 356 4594
Fragments arranged by size:
4594 400 356
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 2621 3413 4797 5065 1632
Size: 705 284 792 1384 268 1917
Fragments arranged by size:
1917 1384 792 705 284 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5153
AflIII A'CryG_T
Cuts at: 1203 4249 5226 1203
Size: 3046 977 1327
Fragments arranged by size:
3046 1327 977
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5350
AlwNI CAG_nnn'CTG
Cuts at: 2078 2090 2174 2423 3093 4665 2078
Size: 12 84 249 670 1572 2763
Fragments arranged by size:
2763 1572 670 249 84 12
ApaLI G'TGCA_C
Cuts at: 3407 4563 3407
Size: 1156 4194
ApoI r'AATT_y
Cuts at: 1493 2429 2659 2885 3037 5146 1493
Size: 936 230 226 152 2109 1697
Fragments arranged by size:
2109 1697 936 230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3904 160
Size: 872 2872 1606
Fragments arranged by size:
2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 1870
Size: 73 732 4545
Fragments arranged by size:
4545 732 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 4126 618
Size: 325 217 627 884 1455 1842
Fragments arranged by size:
1842 1455 884 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2348 2599 2669 1391
Size: 957 251 70 4072
Fragments arranged by size:
4072 957 251 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2249 928
Size: 971 350 4029
Fragments arranged by size:
4029 971 350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2556 2590 3541 3575 1903
Size: 34 50 34 535 34 951 34 3678
Fragments arranged by size:
3678 951 535 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 4452 3242
Size: 1210 4140
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 3961 136
Size: 107 122 71 631 605 490 1799 1525
Fragments arranged by size:
1799 1525 631 605 490 122 107 71
BglII A'GATC_T
Cuts at: 5345 5345
Size: 5350
BlpI GC'TnA_GC
Cuts at: 2767 2767
Size: 5350
Bme1580I G_kGCm'C
Cuts at: 1790 3411 4567 1790
Size: 1621 1156 2573
Fragments arranged by size:
2573 1621 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2261 3081 4039
Size: 416 53 83 399 24 844 820 958
Cuts at: 4039 442
Size: 1753
Fragments arranged by size:
1753 958 844 820 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4010 1239
Size: 2771 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3475 4340 4638 3475
Size: 865 298 4187
Fragments arranged by size:
4187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4013 882
Size: 617 2514 2219
Fragments arranged by size:
2514 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3141 493
Size: 711 1937 2702
Fragments arranged by size:
2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 5344 5344
Size: 5350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3157 3539 4174 275
Size: 53 83 186 936 1624 382 635 1451
Fragments arranged by size:
1624 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2292 3783 4181 4455 4602 5013 2292
Size: 1491 398 274 147 411 2629
Fragments arranged by size:
2629 1491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2464 2494 1178
Size: 30 165 30 1061 30 4034
Fragments arranged by size:
4034 1061 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 1186
Size: 1324 4026
BseYI C'CCAG_C
Cuts at: 2157 2400 4553 5049 2157
Size: 243 2153 496 2458
Fragments arranged by size:
2458 2153 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4777
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3561 4589 1540
Size: 275 186 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3411 3496 4567 3411
Size: 85 1071 4194
Fragments arranged by size:
4194 1071 85
BsmI GAATG_Cn'
Cuts at: 2861 2954 2861
Size: 93 5257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 2599 2669 3411 3496 4567 1391
Size: 399 558 251 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 558 399 251 85 70
BspEI T'CCGG_A
Cuts at: 2292 5013 2292
Size: 2721 2629
BspHI T'CATG_A
Cuts at: 3239 4969 3239
Size: 1730 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 2705 844
Size: 1410 451 3489
Fragments arranged by size:
3489 1410 451
BsrBI CCG'CTC
Cuts at: 1394 1823 3237 1394
Size: 429 1414 3507
Fragments arranged by size:
3507 1414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3845 4019 65
Size: 1183 2597 174 1396
Fragments arranged by size:
2597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3994 4181 1700
Size: 110 2184 187 2869
Fragments arranged by size:
2869 2184 187 110
BsrGI T'GTAC_A
Cuts at: 96 2488 96
Size: 2392 2958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 3410 4422 3410
Size: 1012 4338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 4094
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstYI r'GATC_y
Cuts at: 1972 2680 3047 3432 3449 4901 4987 4999 5345
Size: 708 367 385 17 1452 86 12 346
Cuts at: 5345 1972
Size: 1977
Fragments arranged by size:
1977 1452 708 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4798
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4611
Fragments arranged by size:
4611 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 3660 3680 2866
Size: 794 20 4536
Fragments arranged by size:
4536 794 20
ClaI AT'CG_AT
Cuts at: 1554 2181 1554
Size: 627 4723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2654 2654
Size: 5350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2797 3686 5315
Size: 54 1425 50 275 18 967 889 1629
Cuts at: 5315 8
Size: 43
Fragments arranged by size:
1629 1425 967 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 1537
Size: 275 985 4090
Fragments arranged by size:
4090 985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 2505 3280 1353
Size: 1146 6 775 3423
Fragments arranged by size:
3423 1146 775 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3937 4311 4457 387
Size: 1064 138 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2621 3413 4010 4797 5065 1239
Size: 393 705 284 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 705 597 393 284 268
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
FspI TGC'GCA
Cuts at: 3856 3856
Size: 5350
HaeII r_GCGC'y
Cuts at: 1678 4497 1678
Size: 2819 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2274 2306 3991
Size: 32 135 32 488 32 182 32 1685
Cuts at: 3991 4023 4065 4097 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1685 488 182 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2694 2940 669
Size: 988 281 756 246 3079
Fragments arranged by size:
3079 988 756 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5350
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2949 2949
Size: 5350
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2195 2507 4464 4648 1305
Size: 890 312 1957 184 2007
Fragments arranged by size:
2007 1957 890 312 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3528
Fragments arranged by size:
3528 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3308 3667 3826 518
Size: 648 2142 359 159 2042
Fragments arranged by size:
2142 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2174 2420 2766 3443 4591 4836 1606
Size: 555 13 246 346 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 346 246 245 13
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4798
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2797 2797
Size: 5350
NspI r_CATG'y
Cuts at: 1132 1229 2708 4253 1132
Size: 97 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5350
PciI A'CATG_T
Cuts at: 4249 4249
Size: 5350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5350
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 4932 4964 3419
Size: 32 1481 32 3805
Fragments arranged by size:
3805 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5350
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PstI C_TGCA'G
Cuts at: 830 2702 830
Size: 1872 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
PvuII CAG'CTG
Cuts at: 2161 2174 2420 2161
Size: 13 246 5091
Fragments arranged by size:
5091 246 13
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SfcI C'TryA_G
Cuts at: 826 1045 2037 2698 3833 4514 4705 826
Size: 219 992 661 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 681 661 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2677 1872
Size: 805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3454 4355 4617 820
Size: 197 926 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5350
StyI C'CwwG_G
Cuts at: 513 1065 2789 513
Size: 552 1724 3074
Fragments arranged by size:
3074 1724 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3377 3394 3547 3732 4177 325
Size: 1255 570 1227 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1227 570 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2488 3596 5308
Size: 274 80 33 51 1443 511 1108 1712
Cuts at: 5308 96
Size: 138
Fragments arranged by size:
1712 1443 1108 511 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2464 2496 5310 5342 2464
Size: 32 2814 32 2472
Fragments arranged by size:
2814 2472 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2074 1316
Size: 758 4592
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5350
XmaI C'CCGG_G
Cuts at: 1870 2675 1870
Size: 805 4545
XmnI GAAnn'nnTTC
Cuts at: 3479 3479
Size: 5350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3158 276
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI