Restriction map for
pFN21AB8210
(Circular) (Six-base) MAPSORT of: pFN21AB8210.seq Check: 9457 from: 1 to: 5833
pFN21AB8210 5833 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2708 2852 2972 3142 3368 3520 5629 1493
Size: 1215 144 120 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 1215 226 170 152 144 120
AseI AT'TA_AT
Cuts at: 160 1032 2228 4387 160
Size: 872 1196 2159 1606
Fragments arranged by size:
2159 1606 1196 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4609 618
Size: 325 217 627 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2587 3152 1391
Size: 1196 565 4072
Fragments arranged by size:
4072 1196 565
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BfrBI ATG'CAT
Cuts at: 2116 2116
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2265 4444 136
Size: 107 122 71 631 605 593 2179 1525
Fragments arranged by size:
2179 1525 631 605 593 122 107 71
BglII A'GATC_T
Cuts at: 3120 5828 3120
Size: 2708 3125
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 3894 5050 1790
Size: 2104 1156 2573
Fragments arranged by size:
2573 2104 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2291 1056
Size: 1235 4598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 2568 4493 1239
Size: 1104 225 1925 2579
Fragments arranged by size:
2579 1925 1104 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 2783 5827 2783
Size: 3044 2789
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5833
BseYI C'CCAG_C
Cuts at: 2263 2530 5036 5532 2263
Size: 267 2506 496 2564
Fragments arranged by size:
2564 2506 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 1163
Size: 573 417 4843
Fragments arranged by size:
4843 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 3064 3344 3437 3064
Size: 280 93 5460
Fragments arranged by size:
5460 280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2587 3152 3894 3979 5050 1391
Size: 399 797 565 742 85 1071 2174
Fragments arranged by size:
2174 1071 797 742 565 399 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 2890 3722 5452 2890
Size: 832 1730 3271
Fragments arranged by size:
3271 1730 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2545 2786 2992 3188 844
Size: 1331 370 241 206 196 3489
Fragments arranged by size:
3489 1331 370 241 206 196
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2031 2937 4328 4502 65
Size: 1183 783 906 1391 174 1396
Fragments arranged by size:
1396 1391 1183 906 783 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2692 4477 4664 1700
Size: 110 882 1785 187 2869
Fragments arranged by size:
2869 1785 882 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2702 794
Size: 1908 3925
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2102 2772 3051 3120 3163 3530 3915 3932
Size: 130 670 279 69 43 367 385 17
Cuts at: 3932 5384 5470 5482 5828 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 670 385 367 346 279 130
86 69 43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 3862
Fragments arranged by size:
3862 1419 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2117 487
Size: 683 56 891 4203
Fragments arranged by size:
4203 891 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 3349 4143 4163 2700
Size: 649 794 20 4370
Fragments arranged by size:
4370 794 649 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 2145 3137 2145
Size: 992 4841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 3280 4169
Size: 54 1425 50 275 18 816 634 889
Cuts at: 4169 5798 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 816 634 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3763 1353
Size: 2410 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2568 3896 4493 5280 5548 1239
Size: 393 711 225 1328 597 787 268 1524
Fragments arranged by size:
1524 1328 787 711 597 393 268 225
EcoNI CCTnn'n_nnAGG
Cuts at: 3098 3098
Size: 5833
EcoO109I rG'GnC_Cy
Cuts at: 2534 2534
Size: 5833
EcoRI G'AATT_C
Cuts at: 2852 3142 3520 2852
Size: 290 378 5165
Fragments arranged by size:
5165 378 290
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2411 2443 2872
Size: 32 135 32 725 32 82 32 429
Cuts at: 2872 2904 4474 4506 4548 4580 1373
Size: 32 1570 32 42 32 2626
Fragments arranged by size:
2626 1570 725 429 135 82 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4947 5131 1305
Size: 3642 184 2007
Fragments arranged by size:
3642 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3791 4150 4309 518
Size: 648 2625 359 159 2042
Fragments arranged by size:
2625 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 3249 3926 5074 5319 1606
Size: 657 986 677 1148 245 2120
Fragments arranged by size:
2120 1148 986 677 657 245
NaeI GCC'GGC
Cuts at: 2694 2694
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 3862
Fragments arranged by size:
3862 1419 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NgoMIV G'CCGG_C
Cuts at: 2692 2692
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 2287 1052
Size: 1235 4598
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NsiI A_TGCA'T
Cuts at: 2118 2118
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2093 1348
Size: 745 5088
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2099 3089 1108
Size: 991 990 3852
Fragments arranged by size:
3852 991 990
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2534 2534
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 2868 3403 2868
Size: 535 5298
PstI C_TGCA'G
Cuts at: 830 3185 830
Size: 2355 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2263 2263
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 3181 4316 4997 5188 826
Size: 219 2136 1135 681 191 1471
Fragments arranged by size:
2136 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2484 3272 513
Size: 552 1419 788 3074
Fragments arranged by size:
3074 1419 788 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2897 3860 3877 4030 4215 4660 325
Size: 1255 1317 963 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 963 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3071 4079 5791
Size: 274 80 33 51 1443 1094 1008 1712
Cuts at: 5791 96
Size: 138
Fragments arranged by size:
1712 1443 1094 1008 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI