Restriction map for
pFN21AB8206
(Circular) (Six-base) MAPSORT of: pFN21AB8206.seq Check: 5996 from: 1 to: 5512
pFN21AB8206 5512 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3575 4959 5227 1632
Size: 1943 1384 268 1917
Fragments arranged by size:
1943 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 2333 4343 2333
Size: 2010 3502
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 2544 1166
Size: 1378 4134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 2759 3255 4827 2759
Size: 496 1572 3444
Fragments arranged by size:
3444 1572 496
ApaLI G'TGCA_C
Cuts at: 2742 3569 4725 2742
Size: 827 1156 3529
Fragments arranged by size:
3529 1156 827
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2573 2837 1870
Size: 73 67 563 264 4545
Fragments arranged by size:
4545 563 264 73 67
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2833 4288 618
Size: 325 217 627 272 774 1455 1842
Fragments arranged by size:
1842 1455 774 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2172 2574 2652 2831 1391
Size: 781 402 78 179 4072
Fragments arranged by size:
4072 781 402 179 78
BbeI G_GCGC'C
Cuts at: 2063 2063
Size: 5512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2781 928
Size: 971 882 3659
Fragments arranged by size:
3659 971 882
BbvCI CC'TCA_GC
Cuts at: 2360 2360
Size: 5512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2067 2101 3703 3737 1903
Size: 34 130 34 1602 34 3678
Fragments arranged by size:
3678 1602 130 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 4123 136
Size: 107 122 71 631 605 376 2075 1525
Fragments arranged by size:
2075 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 2719 2769 5507 2719
Size: 50 2738 2724
Fragments arranged by size:
2738 2724 50
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2090 2551 2746 3573 4729 1790
Size: 263 37 461 195 827 1156 2573
Fragments arranged by size:
2573 1156 827 461 263 195 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2804 3243 4201
Size: 416 53 83 399 24 1387 439 958
Cuts at: 4201 442
Size: 1753
Fragments arranged by size:
1753 1387 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2749 2264
Size: 485 5027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2744 4172 1239
Size: 1505 1428 2579
Fragments arranged by size:
2579 1505 1428
Bpu10I CC'TnA_GC
Cuts at: 2360 2360
Size: 5512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 3319 3701 4336
Size: 53 83 186 936 527 1259 382 635
Cuts at: 4336 275
Size: 1451
Fragments arranged by size:
1451 1259 936 635 527 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2333 2639 2669 3945 4343 4617 4764 5175 2333
Size: 306 30 1276 398 274 147 411 2670
Fragments arranged by size:
2670 1276 411 398 306 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5287
Fragments arranged by size:
5287 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2473 1186
Size: 865 422 4225
Fragments arranged by size:
4225 865 422
BseYI C'CCAG_C
Cuts at: 2377 4715 5211 2377
Size: 2338 496 2678
Fragments arranged by size:
2678 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 1163
Size: 573 401 4538
Fragments arranged by size:
4538 573 401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2652 2746 3573 3658 4729 2172
Size: 480 94 827 85 1071 2955
Fragments arranged by size:
2955 1071 827 480 94 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2090 2172 2551 2574 2652 2746
Size: 399 263 37 82 379 23 78 94
Cuts at: 2746 2831 3573 3658 4729 1391
Size: 85 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 263 94 85
85 82 78 37 23
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2480 2867 844
Size: 1636 387 3489
Fragments arranged by size:
3489 1636 387
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2333 4156 4343 1700
Size: 110 523 1823 187 2869
Fragments arranged by size:
2869 1823 523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 2584 1184
Size: 1400 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2318
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2719 2769 2842 3209 3594 3611 5063 5149
Size: 747 50 73 367 385 17 1452 86
Cuts at: 5149 5161 5507 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 747 385 367 346 86 73
50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2311 2778 513
Size: 552 939 307 467 3247
Fragments arranged by size:
3247 939 552 467 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 2339 3028 3822 3842 2128
Size: 211 689 794 20 3798
Fragments arranged by size:
3798 794 689 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2959 3848 5477
Size: 54 1425 50 275 18 1129 889 1629
Cuts at: 5477 8
Size: 43
Fragments arranged by size:
1629 1425 1129 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3442 1353
Size: 2089 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2219 4099 4473 4619 387
Size: 1064 138 630 1880 374 146 1280
Fragments arranged by size:
1880 1280 1064 630 374 146 138
EcoICRI GAG'CTC
Cuts at: 2170 2650 2170
Size: 480 5032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2744 3575 4172 4959 5227 1239
Size: 393 1112 831 597 787 268 1524
Fragments arranged by size:
1524 1112 831 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 2063 4659 1678
Size: 385 2596 2531
Fragments arranged by size:
2596 2531 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4153 4185 4227 4259 1373
Size: 32 135 32 2581 32 42 32 2626
Fragments arranged by size:
2626 2581 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KasI G'GCGC_C
Cuts at: 2059 2059
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2544 3470 3829 3988 518
Size: 648 1378 926 359 159 2042
Fragments arranged by size:
2042 1378 926 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2269 2493 2928 3605 4753 4998 1606
Size: 416 247 224 435 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 435 416 247 245 224
NarI GG'CG_CC
Cuts at: 2060 2060
Size: 5512
NcoI C'CATG_G
Cuts at: 513 1065 2004 2311 2778 513
Size: 552 939 307 467 3247
Fragments arranged by size:
3247 939 552 467 307
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2310 2870 4415 1132
Size: 97 1081 560 1545 2229
Fragments arranged by size:
2229 1545 1081 560 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
PvuII CAG'CTG
Cuts at: 2493 2493
Size: 5512
SacI G_AGCT'C
Cuts at: 2172 2652 2172
Size: 480 5032
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2860 3995 4676 4867 826
Size: 219 1815 1135 681 191 1471
Fragments arranged by size:
1815 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2061 2061
Size: 5512
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2575 2839 1872
Size: 703 264 4545
Fragments arranged by size:
4545 703 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2310 2870 1132
Size: 1178 560 3774
Fragments arranged by size:
3774 1178 560
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 2042 1517
Size: 525 4987
StyI C'CwwG_G
Cuts at: 513 1065 2004 2311 2778 2951 513
Size: 552 939 307 467 173 3074
Fragments arranged by size:
3074 939 552 467 307 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3758 5470 96
Size: 274 80 33 51 1443 1781 1712 138
Fragments arranged by size:
1781 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2505 2537 5472 5504 2505
Size: 32 2935 32 2513
Fragments arranged by size:
2935 2513 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2573 2837 1870
Size: 703 264 4545
Fragments arranged by size:
4545 703 264
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI