Restriction map for
pFN21AB8205
(Circular) (Six-base) MAPSORT of: pFN21AB8205.seq Check: 7325 from: 1 to: 5758
pFN21AB8205 5758 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 2499 2865 3079 2499
Size: 366 214 5178
Fragments arranged by size:
5178 366 214
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3821 5205 5473 1632
Size: 2189 1384 268 1917
Fragments arranged by size:
2189 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2447 1166
Size: 1281 4477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 2845 3005 3501 5073 2845
Size: 160 496 1572 3530
Fragments arranged by size:
3530 1572 496 160
ApaI G_GGCC'C
Cuts at: 2309 2755 2309
Size: 446 5312
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2360 3083 1870
Size: 73 417 723 4545
Fragments arranged by size:
4545 723 417 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2483 2516 2483
Size: 33 5725
BamHI G'GATC_C
Cuts at: 2569 2598 3088 3455 2569
Size: 29 490 367 4872
Fragments arranged by size:
4872 490 367 29
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2124 2145 2153 2449
Size: 325 217 627 221 116 21 8 296
Cuts at: 2449 2499 2514 2760 2865 3079 4534 618
Size: 50 15 246 105 214 1455 1842
Fragments arranged by size:
1842 1455 627 325 296 246 221 217
214 116 105 50 21 15 8
BanII G_rGCy'C
Cuts at: 1391 2067 2309 2346 2755 3077 1391
Size: 676 242 37 409 322 4072
Fragments arranged by size:
4072 676 409 322 242 37
BbeI G_GCGC'C
Cuts at: 2012 2518 2012
Size: 506 5252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2258 4369 136
Size: 107 122 71 631 605 586 2111 1525
Fragments arranged by size:
2111 1525 631 605 586 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2231 2309 2460 2755 3819 4975 1790
Size: 337 104 78 151 295 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 337 295 151 104 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2816 2934 3489
Size: 416 53 83 399 24 1399 118 555
Cuts at: 3489 4447 442
Size: 958 1753
Fragments arranged by size:
1753 1399 958 555 416 399 118 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2369 1056
Size: 1313 4445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2798 2903 4418 1239
Size: 1184 375 105 1515 2579
Fragments arranged by size:
2579 1515 1184 375 105
Bpu10I CC'TnA_GC
Cuts at: 2141 2141
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 4421 882
Size: 617 1413 1509 2219
Fragments arranged by size:
2219 1509 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2103 2515 3565
Size: 53 83 186 936 476 94 412 1050
Cuts at: 3565 3947 4582 275
Size: 382 635 1451
Fragments arranged by size:
1451 1050 936 635 476 412 382 186
94 83 53
BsaWI w'CCGG_w
Cuts at: 2595 4191 4589 4863 5010 5421 2595
Size: 1596 398 274 147 411 2932
Fragments arranged by size:
2932 1596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2102 2420 2928 3023 1186
Size: 877 39 318 508 95 3921
Fragments arranged by size:
3921 877 508 318 95 39
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2784 2853 3819 3904 4975 2784
Size: 69 966 85 1071 3567
Fragments arranged by size:
3567 1071 966 85 69
BsmI GAATG_Cn'
Cuts at: 2275 3269 3362 2275
Size: 994 93 4671
Fragments arranged by size:
4671 994 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2127 2231 2309 2346 2460 2755
Size: 399 277 60 104 78 37 114 295
Cuts at: 2755 2784 2853 3077 3819 3904 4975 1391
Size: 29 69 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 277 224 114
104 85 78 69 60 37 29
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2877 3113 844
Size: 2033 236 3489
Fragments arranged by size:
3489 2033 236
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 4253 4427 65
Size: 1183 1366 1639 174 1396
Fragments arranged by size:
1639 1396 1366 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 4402 4589 1700
Size: 110 201 2391 187 2869
Fragments arranged by size:
2869 2391 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 2576 1184
Size: 1392 4366
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2569 2598 3043 3088 3455 3840 3857 5309
Size: 597 29 445 45 367 385 17 1452
Cuts at: 5309 5395 5407 5753 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 597 445 385 367 346 86
45 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 3006 3006
Size: 5758
BtgI C'CryG_G
Cuts at: 513 1065 2004 2518 513
Size: 552 939 514 3753
Fragments arranged by size:
3753 939 552 514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3274 4068 4088 2503
Size: 771 794 20 4173
Fragments arranged by size:
4173 794 771 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723
Size: 54 1425 50 275 18 1375 889 1629
Cuts at: 5723 8
Size: 43
Fragments arranged by size:
1629 1425 1375 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 1353
Size: 2335 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 4345 4719 4865 387
Size: 1064 138 450 2306 374 146 1280
Fragments arranged by size:
2306 1280 1064 450 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2798 2903 3821 4418 5205 5473
Size: 393 791 375 105 918 597 787 268
Cuts at: 5473 1239
Size: 1524
Fragments arranged by size:
1524 918 791 787 597 393 375 268
105
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2837 2192
Size: 645 5113
EcoO109I rG'GnC_Cy
Cuts at: 2299 2410 2638 3013 2299
Size: 111 228 375 5044
Fragments arranged by size:
5044 375 228 111
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2012 2093 2402 2518 4905 1678
Size: 334 81 309 116 2387 2531
Fragments arranged by size:
2531 2387 334 309 116 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3032 3064 4399 4431 4473
Size: 32 135 32 1460 32 1335 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 1460 1335 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KasI G'GCGC_C
Cuts at: 2008 2514 2008
Size: 506 5252
KpnI G_GTAC'C
Cuts at: 2503 2869 3083 2503
Size: 366 214 5178
Fragments arranged by size:
5178 366 214
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 3716 4075 4234 518
Size: 648 1281 1269 359 159 2042
Fragments arranged by size:
2042 1281 1269 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 2311 3174 3851 4999 5244 1606
Size: 604 101 863 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 604 245 101
NarI GG'CG_CC
Cuts at: 2009 2515 2009
Size: 506 5252
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 2365 1052
Size: 1313 4445
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2818 3116 4661 1132
Size: 97 1589 298 1545 2229
Fragments arranged by size:
2229 1589 1545 298 97
PasI CC'CwG_GG
Cuts at: 1348 2923 1348
Size: 1575 4183
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2638 2299
Size: 339 5419
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2305 2751 2305
Size: 446 5312
PstI C_TGCA'G
Cuts at: 830 2040 2841 2996 3110 830
Size: 1210 801 155 114 3478
Fragments arranged by size:
3478 1210 801 155 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SanDI GG'GwC_CC
Cuts at: 2638 2638
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 2996 3110 2996
Size: 114 5644
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2036 2116 2837 2975 2992 3106 4241
Size: 219 991 80 721 138 17 114 1135
Cuts at: 4241 4922 5113 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 991 721 681 219 191 138
114 80 17
SfoI GGC'GCC
Cuts at: 2010 2516 2010
Size: 506 5252
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 2011 1700
Size: 311 5447
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 2236 152
Size: 2084 3674
SphI G_CATG'C
Cuts at: 1132 2818 3116 1132
Size: 1686 298 3774
Fragments arranged by size:
3774 1686 298
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 2619 1517
Size: 1102 4656
StyI C'CwwG_G
Cuts at: 513 1065 2004 2149 2424 2755 2854 3036 3197
Size: 552 939 145 275 331 99 182 161
Cuts at: 3197 513
Size: 3074
Fragments arranged by size:
3074 939 552 331 275 182 161 145
99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 3785 3802 3955 4140 4585 325
Size: 1255 651 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI