Restriction map for
pFN21AB8203
(Circular) (Six-base) MAPSORT of: pFN21AB8203.seq Check: 837 from: 1 to: 5614
pFN21AB8203 5614 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2793 2793
Size: 5614
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 3677 5061 5329 1632
Size: 605 1440 1384 268 1917
Fragments arranged by size:
1917 1440 1384 605 268
AfeI AGC'GCT
Cuts at: 2416 2441 2416
Size: 25 5589
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 2278 4343 2278
Size: 2065 3549
AleI CACnn'nnGTG
Cuts at: 1166 2559 1166
Size: 1393 4221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 2829
Size: 32 5582
AlwNI CAG_nnn'CTG
Cuts at: 2242 2389 3357 4929 2242
Size: 147 968 1572 2927
Fragments arranged by size:
2927 1572 968 147
ApaI G_GGCC'C
Cuts at: 2590 2590
Size: 5614
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2923 3149 3301 5410 1493
Size: 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 226 152
AscI GG'CGCG_CC
Cuts at: 2257 2257
Size: 5614
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2074 2939 1870
Size: 73 120 11 865 4545
Fragments arranged by size:
4545 865 120 73 11
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 2091 2590 2825 2933 1391
Size: 684 16 499 235 108 4072
Fragments arranged by size:
4072 684 499 235 108 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2087 2121 2755 2789 3805 3839 1903
Size: 34 150 34 634 34 1016 34 3678
Fragments arranged by size:
3678 1016 634 150 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2594 4225 136
Size: 107 122 71 631 605 922 1631 1525
Fragments arranged by size:
1631 1525 922 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2590 3675 4831 1790
Size: 800 1085 1156 2573
Fragments arranged by size:
2573 1156 1085 800
BmgBI CAC'GTC
Cuts at: 1318 2581 1318
Size: 1263 4351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2739 3345 4303
Size: 416 53 83 399 24 1322 606 958
Cuts at: 4303 442
Size: 1753
Fragments arranged by size:
1753 1322 958 606 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2339 2485 4274 1239
Size: 1046 54 146 1789 2579
Fragments arranged by size:
2579 1789 1046 146 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 3739 4604 4902 2075
Size: 1664 865 298 2787
Fragments arranged by size:
2787 1664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4277 882
Size: 617 2778 2219
Fragments arranged by size:
2778 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4047 4445 4719 4866 5277 4047
Size: 398 274 147 411 4384
Fragments arranged by size:
4384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2471 2792 1186
Size: 847 438 321 4008
Fragments arranged by size:
4008 847 438 321
BseYI C'CCAG_C
Cuts at: 2041 2411 2589 2898 4817 5313 2041
Size: 370 178 309 1919 496 2342
Fragments arranged by size:
2342 1919 496 370 309 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2471 1163
Size: 573 735 4306
Fragments arranged by size:
4306 735 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2155 2764 3064 3825 4853 1540
Size: 275 186 154 609 300 761 1028 2301
Fragments arranged by size:
2301 1028 761 609 300 275 186 154
BsiHKAI G_wGCw'C
Cuts at: 2091 2784 3675 3760 4831 2091
Size: 693 891 85 1071 2874
Fragments arranged by size:
2874 1071 891 693 85
BsmI GAATG_Cn'
Cuts at: 2879 3125 3218 2879
Size: 246 93 5275
Fragments arranged by size:
5275 246 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2091 2590 2784 2825 2933 3675
Size: 399 285 16 499 194 41 108 742
Cuts at: 3675 3760 4831 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 285 194 108
85 41 16
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 3503 5233 3503
Size: 1730 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2793 2969 844
Size: 1949 176 3489
Fragments arranged by size:
3489 1949 176
BsrBI CCG'CTC
Cuts at: 1394 1823 2461 3501 1394
Size: 429 638 1040 3507
Fragments arranged by size:
3507 1040 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2508 4109 4283 65
Size: 1183 1260 1601 174 1396
Fragments arranged by size:
1601 1396 1260 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2253 4258 4445 1700
Size: 110 443 2005 187 2869
Fragments arranged by size:
2869 2005 443 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 2079 2257 1925
Size: 154 178 5282
Fragments arranged by size:
5282 178 154
BssSI C'ACGA_G
Cuts at: 2231 2555 3674 4686 2231
Size: 324 1119 1012 3159
Fragments arranged by size:
3159 1119 1012 324
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 2422 4418 2422
Size: 1996 3618
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2944 3311 3696 3713 5165 5251 5263 5609
Size: 972 367 385 17 1452 86 12 346
Cuts at: 5609 1972
Size: 1977
Fragments arranged by size:
1977 1452 972 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 3613
Fragments arranged by size:
3613 1449 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2329 487
Size: 683 56 1103 3772
Fragments arranged by size:
3772 1103 683 56
BtsI GCAGTG_nn'
Cuts at: 2315 3130 3924 3944 2315
Size: 815 794 20 3985
Fragments arranged by size:
3985 815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2579 4621 809
Size: 507 1263 2042 1802
Fragments arranged by size:
2042 1802 1263 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 2181 2244
Size: 54 1425 50 275 18 322 29 63
Cuts at: 2244 2373 3061 3950 5579 8
Size: 129 688 889 1629 43
Fragments arranged by size:
1629 1425 889 688 322 275 129 63
54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2152 3061 1537
Size: 275 340 909 4090
Fragments arranged by size:
4090 909 340 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2424 2532 2660 3544 1353
Size: 710 361 108 128 884 3423
Fragments arranged by size:
3423 884 710 361 128 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2070 2645 4201 4575 4721 387
Size: 1064 138 481 575 1556 374 146 1280
Fragments arranged by size:
1556 1280 1064 575 481 374 146 138
EcoICRI GAG'CTC
Cuts at: 2089 2089
Size: 5614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2285 2339 2485 3677 4274 5061
Size: 393 605 48 54 146 1192 597 787
Cuts at: 5061 5329 1239
Size: 268 1524
Fragments arranged by size:
1524 1192 787 605 597 393 268 146
54 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 2831 2375
Size: 456 5158
EcoO109I rG'GnC_Cy
Cuts at: 2239 2239
Size: 5614
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2728 2311
Size: 417 5197
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 2049 2418 2443 4761 1678
Size: 371 369 25 2318 2531
Fragments arranged by size:
2531 2318 371 369 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 4255 4287 4329
Size: 32 135 32 692 32 1959 32 42
Cuts at: 4329 4361 1373
Size: 32 2626
Fragments arranged by size:
2626 1959 692 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3249
Fragments arranged by size:
3249 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 3572 3931 4090 518
Size: 648 1393 1013 359 159 2042
Fragments arranged by size:
2042 1393 1013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2317 2386 2516 3030 3707 4855 5100
Size: 570 141 69 130 514 677 1148 245
Cuts at: 5100 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 570 514 245 141 130
69
NaeI GCC'GGC
Cuts at: 2255 2255
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5062
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NgoMIV G'CCGG_C
Cuts at: 2253 2253
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NruI TCG'CGA
Cuts at: 2429 2429
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2972 4517 1132
Size: 97 1743 1545 2229
Fragments arranged by size:
2229 1743 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5614
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PspOMI G'GGCC_C
Cuts at: 2586 2586
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2358 2790 2966 830
Size: 1528 432 176 3478
Fragments arranged by size:
3478 1528 432 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2317 2317
Size: 5614
SacI G_AGCT'C
Cuts at: 2091 2091
Size: 5614
SacII CC_GC'GG
Cuts at: 2517 2517
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SapI GCTCTTCn'nnn_
Cuts at: 2063 2424 2063
Size: 361 5253
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2354 2786 2962 4097 4778 4969 826
Size: 219 1309 432 176 1135 681 191 1471
Fragments arranged by size:
1471 1309 1135 681 432 219 191 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 2594 2594
Size: 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 3718 4619 4881 820
Size: 197 926 147 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 4534
StyI C'CwwG_G
Cuts at: 513 1065 2376 3053 513
Size: 552 1311 677 3074
Fragments arranged by size:
3074 1311 677 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2890 3641 3658 3811 3996 4441 325
Size: 1255 1310 751 17 153 185 445 1498
Fragments arranged by size:
1498 1310 1255 751 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 3860 5572
Size: 274 80 33 51 1443 645 1238 1712
Cuts at: 5572 96
Size: 138
Fragments arranged by size:
1712 1443 1238 645 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NarI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SanDI SexAI SfoI SrfI SwaI XcmI