Restriction map for
pFN21AB8195
(Circular) (Six-base) MAPSORT of: pFN21AB8195.seq Check: 3794 from: 1 to: 5644
pFN21AB8195 5644 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 3707 5091 5359 1632
Size: 1004 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1071 1004 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2231 1184
Size: 1047 4597
AlwNI CAG_nnn'CTG
Cuts at: 2047 2119 2176 2227 3387 4959 2047
Size: 72 57 51 1160 1572 2732
Fragments arranged by size:
2732 1572 1160 72 57 51
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2789 2965 4420 618
Size: 325 217 627 1002 176 1455 1842
Fragments arranged by size:
1842 1455 1002 627 325 217 176
BanII G_rGCy'C
Cuts at: 1391 2237 2364 2963 1391
Size: 846 127 599 4072
Fragments arranged by size:
4072 846 599 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 928
Size: 971 255 4418
Fragments arranged by size:
4418 971 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 3705 4861 1790
Size: 1915 1156 2573
Fragments arranged by size:
2573 1915 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2497 2582 4304 1239
Size: 902 356 85 1722 2579
Fragments arranged by size:
2579 1722 902 356 85
Bpu10I CC'TnA_GC
Cuts at: 2088 2888 2088
Size: 800 4844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 3769 4634 4932 2585
Size: 1184 865 298 3297
Fragments arranged by size:
3297 1184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2530 3435 493
Size: 711 1326 905 2702
Fragments arranged by size:
2702 1326 905 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2912 3451 3833 4468
Size: 53 83 186 936 1379 539 382 635
Cuts at: 4468 275
Size: 1451
Fragments arranged by size:
1451 1379 936 635 539 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2901 4077 4475 4749 4896 5307 2901
Size: 1176 398 274 147 411 3238
Fragments arranged by size:
3238 1176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 1178
Size: 30 165 30 711 30 4678
Fragments arranged by size:
4678 711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 2273 1186
Size: 1059 28 4557
Fragments arranged by size:
4557 1059 28
BseYI C'CCAG_C
Cuts at: 2368 2633 2847 2915 4847 5343 2368
Size: 265 214 68 1932 496 2669
Fragments arranged by size:
2669 1932 496 265 214 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 1163
Size: 573 723 4348
Fragments arranged by size:
4348 723 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 3705 3790 4861 2364
Size: 1341 85 1071 3147
Fragments arranged by size:
3147 1341 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2364 2963 3705 3790 4861 1391
Size: 399 447 127 599 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 599 447 399 127 85
BspEI T'CCGG_A
Cuts at: 2901 5307 2901
Size: 2406 3238
BspHI T'CATG_A
Cuts at: 2694 3533 5263 2694
Size: 839 1730 3075
Fragments arranged by size:
3075 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 3531 1394
Size: 429 629 1079 3507
Fragments arranged by size:
3507 1079 629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2669 4288 4475 1700
Size: 110 859 1619 187 2869
Fragments arranged by size:
2869 1619 859 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 2708 4448 2708
Size: 1740 3904
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2898 2974 3341 3726 3743 5195 5281 5293
Size: 926 76 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 926 385 367 346 86 76
17 12
Bsu36I CC'TnA_GG
Cuts at: 2228 2228
Size: 5644
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2538 2640 513
Size: 552 939 198 336 102 3517
Fragments arranged by size:
3517 939 552 336 198 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2650 487
Size: 683 56 1424 3481
Fragments arranged by size:
3481 1424 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2590 4651 809
Size: 507 1274 2061 1802
Fragments arranged by size:
2061 1802 1274 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2814 3091 3980
Size: 54 1425 50 275 18 984 277 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 984 889 277 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2497 2582 2636 3707 4304 5091
Size: 393 509 356 85 54 1071 597 787
Cuts at: 5091 5359 1239
Size: 268 1524
Fragments arranged by size:
1524 1071 787 597 509 393 356 268
85 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2491 2825 2491
Size: 334 5310
EcoO109I rG'GnC_Cy
Cuts at: 2071 2224 2629 2071
Size: 153 405 5086
Fragments arranged by size:
5086 405 153
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2023 2284 2510 2922 4791 1678
Size: 345 261 226 412 1869 2531
Fragments arranged by size:
2531 1869 412 345 261 226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2890 2922 4285 4317 4359
Size: 32 135 32 1318 32 1363 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1363 1318 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 2888 4758 4942 1305
Size: 1314 269 1870 184 2007
Fragments arranged by size:
2007 1870 1314 269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2816 10
Size: 54 1425 343 984 2838
Fragments arranged by size:
2838 1425 984 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 3602 3961 4120 518
Size: 648 1533 903 359 159 2042
Fragments arranged by size:
2042 1533 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4153
Fragments arranged by size:
4153 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 1108
Size: 1011 4633
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2530 2530
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2224
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2407 2476 2992 4127 4808 4999 826
Size: 219 1362 69 516 1135 681 191 1471
Fragments arranged by size:
1471 1362 1135 681 516 219 191 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 3748 4649 4911 820
Size: 197 926 657 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 901 657 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 2486 1517
Size: 969 4675
StyI C'CwwG_G
Cuts at: 513 1065 2004 2775 3083 513
Size: 552 939 771 308 3074
Fragments arranged by size:
3074 939 771 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 2647 2116
Size: 531 5113
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI