Restriction map for
pFN21AB8191
(Circular) (Six-base) MAPSORT of: pFN21AB8191.seq Check: 4218 from: 1 to: 5980
pFN21AB8191 5980 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2221 2221
Size: 5980
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3093 3323 1937
Size: 1156 230 4594
Fragments arranged by size:
4594 1156 230
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3044 4043 5427 5695 1632
Size: 1412 999 1384 268 1917
Fragments arranged by size:
1917 1412 1384 999 268
AfeI AGC'GCT
Cuts at: 2807 2938 2972 2807
Size: 131 34 5815
Fragments arranged by size:
5815 131 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 3723 5295 3723
Size: 1572 4408
ApaLI G'TGCA_C
Cuts at: 3112 4037 5193 3112
Size: 925 1156 3899
Fragments arranged by size:
3899 1156 925
ApoI r'AATT_y
Cuts at: 1493 3289 3515 3667 5776 1493
Size: 1796 226 152 2109 1697
Fragments arranged by size:
2109 1796 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3305 1870
Size: 73 1362 4545
Fragments arranged by size:
4545 1362 73
AvrII C'CTAG_G
Cuts at: 2815 2815
Size: 5980
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2422 2982 3301 4756 618
Size: 325 217 627 635 560 319 1455 1842
Fragments arranged by size:
1842 1455 635 627 560 325 319 217
BanII G_rGCy'C
Cuts at: 1391 2253 2520 3251 3299 1391
Size: 862 267 731 48 4072
Fragments arranged by size:
4072 862 731 267 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2141 2905 928
Size: 971 242 764 4003
Fragments arranged by size:
4003 971 764 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3112 3146 4171 4205 1903
Size: 34 1175 34 1025 34 3678
Fragments arranged by size:
3678 1175 1025 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 3059 4591
Size: 107 122 71 631 605 380 1007 1532
Cuts at: 4591 136
Size: 1525
Fragments arranged by size:
1532 1525 1007 631 605 380 122 107
71
BglII A'GATC_T
Cuts at: 3027 5975 3027
Size: 2948 3032
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 3116 4041 5197 1790
Size: 1326 925 1156 2573
Fragments arranged by size:
2573 1326 1156 925
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3058 3711 4669
Size: 416 53 83 399 24 1641 653 958
Cuts at: 4669 442
Size: 1753
Fragments arranged by size:
1753 1641 958 653 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 4640 1239
Size: 938 2463 2579
Fragments arranged by size:
2579 2463 938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 4105 4970 5268 2114
Size: 1991 865 298 2826
Fragments arranged by size:
2826 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 4643 882
Size: 617 1032 2112 2219
Fragments arranged by size:
2219 2112 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 2895 2925 1178
Size: 30 165 30 675 30 787 30 4233
Fragments arranged by size:
4233 787 675 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 5067
BseYI C'CCAG_C
Cuts at: 2050 2644 3141 5183 5679 2050
Size: 594 497 2042 496 2351
Fragments arranged by size:
2351 2042 594 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 3182 1163
Size: 573 543 903 3961
Fragments arranged by size:
3961 903 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2448 3430 4191 5219 1540
Size: 275 186 36 411 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 411 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2705 3116 4041 4126 5197 2705
Size: 411 925 85 1071 3488
Fragments arranged by size:
3488 1071 925 411 85
BsmI GAATG_Cn'
Cuts at: 2848 3491 3584 2848
Size: 643 93 5244
Fragments arranged by size:
5244 643 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2520 2705 3116 3251 3299 4041
Size: 399 463 267 185 411 135 48 742
Cuts at: 4041 4126 5197 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 411 399 267 185
135 85 48
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2221 2301 2600 2673 3335 844
Size: 1377 80 299 73 662 3489
Fragments arranged by size:
3489 1377 662 299 80 73
BsrBI CCG'CTC
Cuts at: 1394 1823 3149 3867 1394
Size: 429 1326 718 3507
Fragments arranged by size:
3507 1326 718 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2223 2689 4624 4811 1700
Size: 110 413 466 1935 187 2869
Fragments arranged by size:
2869 1935 466 413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 2306 4040 5052 2306
Size: 1734 1012 3234
Fragments arranged by size:
3234 1734 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2773 2975 2215
Size: 558 202 5220
Fragments arranged by size:
5220 558 202
BstYI r'GATC_y
Cuts at: 1972 2439 3003 3027 3310 3677 4062 4079 5531
Size: 467 564 24 283 367 385 17 1452
Cuts at: 5531 5617 5629 5975 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 564 467 385 367 346 283
86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2319 513
Size: 552 1254 4174
Fragments arranged by size:
4174 1254 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2296 487
Size: 683 56 1070 4171
Fragments arranged by size:
4171 1070 683 56
BtsI GCAGTG_nn'
Cuts at: 2856 3224 3496 4290 4310 2856
Size: 368 272 794 20 4526
Fragments arranged by size:
4526 794 368 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 2193 2193
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2285 2445
Size: 54 1425 50 275 18 391 64 160
Cuts at: 2445 2988 3427 4316 5945 8
Size: 543 439 889 1629 43
Fragments arranged by size:
1629 1425 889 543 439 391 275 160
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2445 3427 1537
Size: 275 633 982 4090
Fragments arranged by size:
4090 982 633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2872 3910 1353
Size: 1519 1038 3423
Fragments arranged by size:
3423 1519 1038
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 3044 4043 4640 5427 5695 1239
Size: 393 545 867 999 597 787 268 1524
Fragments arranged by size:
1524 999 867 787 597 545 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2211 3232 2211
Size: 1021 4959
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2047 2809 2940 2974 5127 1678
Size: 369 762 131 34 2153 2531
Fragments arranged by size:
2531 2153 762 369 131 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2895 2927 4621 4653 4695
Size: 32 135 32 1323 32 1694 32 42
Cuts at: 4695 4727 1373
Size: 32 2626
Fragments arranged by size:
2626 1694 1323 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2185 2836 3073 3102 3324 3570
Size: 988 281 247 651 237 29 222 246
Cuts at: 3570 669
Size: 3079
Fragments arranged by size:
3079 988 651 281 247 246 237 222
29
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2287 10
Size: 54 1425 343 455 3703
Fragments arranged by size:
3703 1425 455 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2771 3013 3122 3204 3242 3938 4297
Size: 648 1605 242 109 82 38 696 359
Cuts at: 4297 4456 518
Size: 159 2042
Fragments arranged by size:
2042 1605 696 648 359 242 159 109
82 38
MspA1I CmG'CkG
Cuts at: 1606 2146 3264 3396 4073 5221 5466 1606
Size: 540 1118 132 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 677 540 245 132
NaeI GCC'GGC
Cuts at: 2691 2691
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2319 513
Size: 552 1254 4174
Fragments arranged by size:
4174 1254 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 2689 2689
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2549 2602 1108
Size: 1441 53 4486
Fragments arranged by size:
4486 1441 53
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2211 3232 2211
Size: 1021 4959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 2670 3332 830
Size: 1840 662 3478
Fragments arranged by size:
3478 1840 662
PvuI CG_AT'CG
Cuts at: 2001 2037 2001
Size: 36 5944
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2470 2666 3328 4463 5144 5335 826
Size: 219 1425 196 662 1135 681 191 1471
Fragments arranged by size:
1471 1425 1135 681 662 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 4084 4985 5247 820
Size: 197 926 186 1955 901 262 1553
Fragments arranged by size:
1955 1553 926 901 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 2530 3904 5
Size: 47 2478 1374 2081
Fragments arranged by size:
2478 2081 1374 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2319 2815 3419 513
Size: 552 1254 496 604 3074
Fragments arranged by size:
3074 1254 604 552 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3058 3254 4007 4024 4177 4362 4807
Size: 1255 1478 196 753 17 153 185 445
Cuts at: 4807 325
Size: 1498
Fragments arranged by size:
1498 1478 1255 753 445 196 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3153 4226 5938
Size: 274 80 33 51 1443 1176 1073 1712
Cuts at: 5938 96
Size: 138
Fragments arranged by size:
1712 1443 1176 1073 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2868 2900 5940 5972 2078
Size: 32 758 32 3040 32 2086
Fragments arranged by size:
3040 2086 758 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2360 3230 1316
Size: 1044 870 4066
Fragments arranged by size:
4066 1044 870
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 3029 4109 3029
Size: 1080 4900
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI