Restriction map for
pFN21AB8187
(Circular) (Six-base) MAPSORT of: pFN21AB8187.seq Check: 2680 from: 1 to: 6061
pFN21AB8187 6061 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2794 3070 2794
Size: 276 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3172 3382 3172
Size: 210 5851
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 3120 4124 5508 5776 1632
Size: 1058 430 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1058 1004 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 3126 4960 5937 1203
Size: 1923 1834 977 1327
Fragments arranged by size:
1923 1834 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2388 3804 5376 2388
Size: 1416 1572 3073
Fragments arranged by size:
3073 1572 1416
ApaI G_GGCC'C
Cuts at: 2377 2377
Size: 6061
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 3317 3370 3596 3748 5857 1493
Size: 1824 53 226 152 2109 1697
Fragments arranged by size:
2109 1824 1697 226 152 53
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 2828 3391 3758 2828
Size: 563 367 5131
Fragments arranged by size:
5131 563 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2428 2768 2855 3172 3382
Size: 325 217 627 641 340 87 317 210
Cuts at: 3382 4837 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 641 627 340 325 317 217
210 87
BanII G_rGCy'C
Cuts at: 1391 2377 2943 3380 1391
Size: 986 566 437 4072
Fragments arranged by size:
4072 986 566 437
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3108 3303 928
Size: 971 1209 195 3686
Fragments arranged by size:
3686 1209 971 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2026 2060 2845 2879 4252 4286 1903
Size: 34 89 34 785 34 1373 34 3678
Fragments arranged by size:
3678 1373 785 89 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 2163 1548
Size: 615 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 2136 4672
Size: 107 122 71 631 605 362 102 2536
Cuts at: 4672 136
Size: 1525
Fragments arranged by size:
2536 1525 631 605 362 122 107 102
71
BglII A'GATC_T
Cuts at: 3282 6056 3282
Size: 2774 3287
BlpI GC'TnA_GC
Cuts at: 3118 3478 3118
Size: 360 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2179 2313 2377 4122 5278 1790
Size: 389 134 64 1745 1156 2573
Fragments arranged by size:
2573 1745 1156 389 134 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2131 2753 3792
Size: 416 53 83 399 24 714 622 1039
Cuts at: 3792 4750 442
Size: 958 1753
Fragments arranged by size:
1753 1039 958 714 622 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2462 2629 2158
Size: 304 167 5590
Fragments arranged by size:
5590 304 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2637 2704 4721 1239
Size: 1398 67 2017 2579
Fragments arranged by size:
2579 2017 1398 67
Bpu10I CC'TnA_GC
Cuts at: 2729 2729
Size: 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3362 4186 5051 5349 3362
Size: 824 865 298 4074
Fragments arranged by size:
4074 865 824 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 2162 6055 2162
Size: 3893 2168
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2781 1186
Size: 1595 4466
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 4924
Fragments arranged by size:
4924 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2757 4122 4207 5278 2757
Size: 1365 85 1071 3540
Fragments arranged by size:
3540 1365 1071 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2313 2377 2757 2943 3380 4122
Size: 399 389 134 64 380 186 437 742
Cuts at: 4122 4207 5278 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 399 389 380 186
134 85 64
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3276 3950 5680 3276
Size: 674 1730 3657
Fragments arranged by size:
3657 1730 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2794 3070 3416 844
Size: 1950 276 346 3489
Fragments arranged by size:
3489 1950 346 276
BsrBI CCG'CTC
Cuts at: 1394 1823 2142 2455 3948 1394
Size: 429 319 313 1493 3507
Fragments arranged by size:
3507 1493 429 319 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 2653 4556 4730 65
Size: 1183 1405 1903 174 1396
Fragments arranged by size:
1903 1405 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2251 96
Size: 2155 3906
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 3044 3044
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2828 3282 3391 3758 4143 4160 5612 5698
Size: 856 454 109 367 385 17 1452 86
Cuts at: 5698 5710 6056 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 856 454 385 367 346 109
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 513
Size: 552 939 193 4377
Fragments arranged by size:
4377 939 552 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 3147 3577 4371 4391 2295
Size: 852 430 794 20 3965
Fragments arranged by size:
3965 852 794 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 2025 2025
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026
Size: 54 1425 50 275 18 1678 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1678 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2690 2709 3991 1353
Size: 1337 19 1282 3423
Fragments arranged by size:
3423 1337 1282 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2637 2690 2704 3120 4124 4721 5508
Size: 393 1005 53 14 416 1004 597 787
Cuts at: 5508 5776 1239
Size: 268 1524
Fragments arranged by size:
1524 1005 1004 787 597 416 393 268
53 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 6061
EcoO109I rG'GnC_Cy
Cuts at: 2356 2373 2374 2356
Size: 17 1 6043
Fragments arranged by size:
6043 17 1
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2946 2978 4702 4734 4776
Size: 32 135 32 1374 32 1724 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 1724 1374 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3176 3386 3176
Size: 210 5851
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 2657 2748 3329 4019 4378 4537
Size: 648 1138 353 91 581 690 359 159
Cuts at: 4537 518
Size: 2042
Fragments arranged by size:
2042 1138 690 648 581 359 353 159
91
MspA1I CmG'CkG
Cuts at: 1606 2637 3477 4154 5302 5547 1606
Size: 1031 840 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 840 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
NdeI CA'TA_TG
Cuts at: 387 2824 387
Size: 2437 3624
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2960 3055 3130 3419 4964 1132
Size: 97 1731 95 75 289 1545 2229
Fragments arranged by size:
2229 1731 1545 289 97 95 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 3126 4960 3126
Size: 1834 4227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 4130 4162 5643 5675 2543
Size: 32 1555 32 1481 32 2929
Fragments arranged by size:
2929 1555 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2356 2356
Size: 6061
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2373 2373
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3090 3413 830
Size: 2260 323 3478
Fragments arranged by size:
3478 2260 323
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SapI GCTCTTCn'nnn_
Cuts at: 2690 2690
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SexAI A'CCwGG_T
Cuts at: 2358 3247 2358
Size: 889 5172
SfcI C'TryA_G
Cuts at: 826 1045 3086 3409 4544 5225 5416 826
Size: 219 2041 323 1135 681 191 1471
Fragments arranged by size:
2041 1471 1135 681 323 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3341 4165 5066 5328 820
Size: 197 926 1398 824 901 262 1553
Fragments arranged by size:
1553 1398 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 3228 1517
Size: 1711 4350
StyI C'CwwG_G
Cuts at: 513 1065 2004 2424 3500 513
Size: 552 939 420 1076 3074
Fragments arranged by size:
3074 1076 939 552 420
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 2268 2687
Size: 274 80 33 51 1443 274 17 419
Cuts at: 2687 2842 4307 6019 96
Size: 155 1465 1712 138
Fragments arranged by size:
1712 1465 1443 419 274 274 155 138
80 51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3198 3230 6021 6053 3198
Size: 32 2791 32 3206
Fragments arranged by size:
3206 2791 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2654 1316
Size: 1338 4723
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 2866
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 3228 4190 3228
Size: 962 5099
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI