Restriction map for
pFN21AB8186
(Circular) (Six-base) MAPSORT of: pFN21AB8186.seq Check: 279 from: 1 to: 5614
pFN21AB8186 5614 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 2087 4445 2087
Size: 2358 3256
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2768 1184
Size: 1584 4030
AlwNI CAG_nnn'CTG
Cuts at: 2776 3357 4929 2776
Size: 581 1572 3461
Fragments arranged by size:
3461 1572 581
ApaLI G'TGCA_C
Cuts at: 2641 3671 4827 2641
Size: 1030 1156 3428
Fragments arranged by size:
3428 1156 1030
ApoI r'AATT_y
Cuts at: 1493 2045 2192 2265 2817 2923 3149 3301 5410
Size: 552 147 73 552 106 226 152 2109
Cuts at: 5410 1493
Size: 1697
Fragments arranged by size:
2109 1697 552 552 226 152 147 106
73
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2935 4390 618
Size: 325 217 627 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2933 1391
Size: 1542 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2264 928
Size: 971 365 4278
Fragments arranged by size:
4278 971 365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2137 2171 2430 2464 3805 3839 1903
Size: 34 200 34 259 34 1341 34 3678
Fragments arranged by size:
3678 1341 259 200 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 4225 136
Size: 107 122 71 631 605 473 2080 1525
Fragments arranged by size:
2080 1525 631 605 473 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2394 2645 3675 4831 1790
Size: 604 251 1030 1156 2573
Fragments arranged by size:
2573 1156 1030 604 251
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2388 2597 2708
Size: 416 53 83 399 24 971 209 111
Cuts at: 2708 3345 4303 442
Size: 637 958 1753
Fragments arranged by size:
1753 971 958 637 416 399 209 111
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4274 1239
Size: 3035 2579
Bpu10I CC'TnA_GC
Cuts at: 2568 2568
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3739 4604 4902 3739
Size: 865 298 4451
Fragments arranged by size:
4451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4277 882
Size: 617 2778 2219
Fragments arranged by size:
2778 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3405 493
Size: 711 918 1283 2702
Fragments arranged by size:
2702 1283 918 711
BsaBI GATnn'nnATC
Cuts at: 5608 5608
Size: 5614
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3421 3803 4438 275
Size: 53 83 186 936 1888 382 635 1451
Fragments arranged by size:
1888 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2087 4047 4445 4719 4866 5277 2087
Size: 1960 398 274 147 411 2424
Fragments arranged by size:
2424 1960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5389
Fragments arranged by size:
5389 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2427 2448 1186
Size: 856 385 21 4352
Fragments arranged by size:
4352 856 385 21
BseYI C'CCAG_C
Cuts at: 2470 4817 5313 2470
Size: 2347 496 2771
Fragments arranged by size:
2771 2347 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5041
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3064 3825 4853 1540
Size: 275 186 1063 761 1028 2301
Fragments arranged by size:
2301 1063 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2645 3675 3760 4831 2645
Size: 1030 85 1071 3428
Fragments arranged by size:
3428 1071 1030 85
BsiWI C'GTAC_G
Cuts at: 2838 2838
Size: 5614
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2645 2933 3675 3760 4831 1391
Size: 399 604 251 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 288 251 85
BspEI T'CCGG_A
Cuts at: 5277 5277
Size: 5614
BspHI T'CATG_A
Cuts at: 2425 3503 5233 2425
Size: 1078 1730 2806
Fragments arranged by size:
2806 1730 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2775 2969 844
Size: 1931 194 3489
Fragments arranged by size:
3489 1931 194
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4109 4283 65
Size: 1183 2861 174 1396
Fragments arranged by size:
2861 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2087 4258 4445 1700
Size: 110 277 2171 187 2869
Fragments arranged by size:
2869 2171 277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 3674 4686 3674
Size: 1012 4602
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2526 2776 794
Size: 1732 250 3632
Fragments arranged by size:
3632 1732 250
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2106 2250 2944 3311 3696 3713 5165 5251
Size: 134 144 694 367 385 17 1452 86
Cuts at: 5251 5263 5609 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 694 385 367 346 144 134
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2011 2805 513
Size: 552 939 7 794 3322
Fragments arranged by size:
3322 939 794 552 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2400 487
Size: 683 56 1174 3701
Fragments arranged by size:
3701 1174 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2781 3061 3950
Size: 54 1425 50 275 18 951 280 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 951 889 280 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3061 1537
Size: 275 1249 4090
Fragments arranged by size:
4090 1249 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4201 4575 4721 387
Size: 1064 138 2612 374 146 1280
Fragments arranged by size:
2612 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3677 4274 5061 5329 1239
Size: 393 2045 597 787 268 1524
Fragments arranged by size:
2045 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2923 3301 2923
Size: 378 5236
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 4761 1678
Size: 3083 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373
Size: 32 135 32 2683 32 42 32 2626
Fragments arranged by size:
2683 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2958 3204 669
Size: 988 281 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2459 748
Size: 1711 3903
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2656 4728 4912 1305
Size: 1351 2072 184 2007
Fragments arranged by size:
2072 2007 1351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 3572 3931 4090 518
Size: 648 1002 1404 359 159 2042
Fragments arranged by size:
2042 1404 1002 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2037 3030 3707 4855 5100 1606
Size: 407 24 993 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 677 407 245 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 2805 513
Size: 552 939 801 3322
Fragments arranged by size:
3322 939 801 552
NdeI CA'TA_TG
Cuts at: 387 2305 387
Size: 1918 3696
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 3061 3061
Size: 5614
NspI r_CATG'y
Cuts at: 1132 1229 2972 4517 1132
Size: 97 1743 1545 2229
Fragments arranged by size:
2229 1743 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5614
PfoI T'CCnGG_A
Cuts at: 2328 2328
Size: 5614
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 2537 3184 2537
Size: 647 4967
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 5582
PstI C_TGCA'G
Cuts at: 830 2453 2727 2757 2772 2966 830
Size: 1623 274 30 15 194 3478
Fragments arranged by size:
3478 1623 274 194 30 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2037 2037
Size: 5614
SacII CC_GC'GG
Cuts at: 2014 2014
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 3862 5574 3862
Size: 1712 3902
SfcI C'TryA_G
Cuts at: 826 1045 2449 2723 2753 2768 2962 4097 4778
Size: 219 1404 274 30 15 194 1135 681
Cuts at: 4778 4969 826
Size: 191 1471
Fragments arranged by size:
1471 1404 1135 681 274 219 194 191
30 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3718 4619 4881 820
Size: 197 926 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2122 3405 493
Size: 1629 1283 2702
Fragments arranged by size:
2702 1629 1283
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 2127 3538 5
Size: 47 2075 1411 2081
Fragments arranged by size:
2081 2075 1411 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 2589 2805 3053 513
Size: 552 939 18 567 216 248 3074
Fragments arranged by size:
3074 939 567 552 248 216 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2229 3641 3658 3811 3996 4441 325
Size: 1255 649 1412 17 153 185 445 1498
Fragments arranged by size:
1498 1412 1255 649 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 2337 3860
Size: 274 80 33 51 1443 331 29 1523
Cuts at: 3860 5572 96
Size: 1712 138
Fragments arranged by size:
1712 1523 1443 331 274 138 80 51
33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5574 5606 5574
Size: 32 5582
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 2045 3743 2045
Size: 1698 3916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI