Restriction map for
pFN21AB8184
(Circular) (Six-base) MAPSORT of: pFN21AB8184.seq Check: 5682 from: 1 to: 5671
pFN21AB8184 5671 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2585 2672 2585
Size: 87 5584
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2540 3734 5118 5386 1632
Size: 908 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 908 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2415 1166
Size: 1249 4422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2514 1184
Size: 1330 4341
AlwNI CAG_nnn'CTG
Cuts at: 2336 3414 4986 2336
Size: 1078 1572 3021
Fragments arranged by size:
3021 1572 1078
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2879 2980 3206 3358 5467 1493
Size: 1386 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2996 1870
Size: 73 228 825 4545
Fragments arranged by size:
4545 825 228 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 2992 4447 618
Size: 325 217 627 810 395 1455 1842
Fragments arranged by size:
1842 1455 810 627 395 325 217
BanII G_rGCy'C
Cuts at: 1391 2208 2990 1391
Size: 817 782 4072
Fragments arranged by size:
4072 817 782
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2546 2580 3862 3896 1903
Size: 34 609 34 1282 34 3678
Fragments arranged by size:
3678 1282 609 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 2324 2812
Size: 107 122 71 631 605 478 174 488
Cuts at: 2812 4282 136
Size: 1470 1525
Fragments arranged by size:
1525 1470 631 605 488 478 174 122
107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 2438 3088 2438
Size: 650 5021
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2945 4331 1239
Size: 1706 1386 2579
Fragments arranged by size:
2579 1706 1386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2958 4104 4502 4776 4923 5334 2958
Size: 1146 398 274 147 411 3295
Fragments arranged by size:
3295 1146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 4874 5370 4874
Size: 496 5175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 2552 2693 2857 1163
Size: 573 403 413 141 164 3977
Fragments arranged by size:
3977 573 413 403 164 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3732 3817 4888 3732
Size: 85 1071 4515
Fragments arranged by size:
4515 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2990 3732 3817 4888 1391
Size: 399 418 782 742 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 418 399 85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 2672 3026 844
Size: 1741 87 354 3489
Fragments arranged by size:
3489 1741 354 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2190 2229 2239 2452 3558 1394
Size: 429 367 39 10 213 1106 3507
Fragments arranged by size:
3507 1106 429 367 213 39 10
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2811 4315 4502 1700
Size: 110 226 775 1504 187 2869
Fragments arranged by size:
2869 1504 775 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 2703 2802 1925
Size: 778 99 4794
Fragments arranged by size:
4794 778 99
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2336 2826 794
Size: 1542 490 3639
Fragments arranged by size:
3639 1542 490
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2464 2383
Size: 81 5590
BstYI r'GATC_y
Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666
Size: 1029 367 385 17 1452 86 12 346
Cuts at: 5666 1972
Size: 1977
Fragments arranged by size:
1977 1452 1029 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2376 2457 513
Size: 552 1311 81 3727
Fragments arranged by size:
3727 1311 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2083 2092 2127 2272 2319 2840 3187 3981 4001
Size: 9 35 145 47 521 347 794 20
Cuts at: 4001 2083
Size: 3753
Fragments arranged by size:
3753 794 521 347 145 47 35 20
9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2373 2427
Size: 54 1425 50 275 18 519 24 54
Cuts at: 2427 2614 2694 2863 3118 4007 5636 8
Size: 187 80 169 255 889 1629 43
Fragments arranged by size:
1629 1425 889 519 275 255 187 169
80 54 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2257 2669 3601 1353
Size: 904 412 932 3423
Fragments arranged by size:
3423 932 904 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2313 4258 4632 4778 387
Size: 1064 138 724 1945 374 146 1280
Fragments arranged by size:
1945 1280 1064 724 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2540 2945 3734 4331 5118 5386 1239
Size: 393 908 405 789 597 787 268 1524
Fragments arranged by size:
1524 908 789 787 597 405 393 268
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 2502 4177 2502
Size: 1675 3996
HaeII r_GCGC'y
Cuts at: 1678 2643 4818 1678
Size: 965 2175 2531
Fragments arranged by size:
2531 2175 965
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2851 2883 4312 4344 4386
Size: 32 135 32 1279 32 1429 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 1429 1279 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2159 2646 2966 4785 4969 1305
Size: 854 487 320 1819 184 2007
Fragments arranged by size:
2007 1819 854 487 320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 2429 2616 2865 10
Size: 54 1425 343 519 78 187 249 2816
Fragments arranged by size:
2816 1425 519 343 249 187 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2415 2513 3629 3988 4147 518
Size: 648 1215 34 98 1116 359 159 2042
Fragments arranged by size:
2042 1215 1116 648 359 159 98 34
MspA1I CmG'CkG
Cuts at: 1606 2070 2336 2482 2548 2918 3087 3764 4912
Size: 464 266 146 66 370 169 677 1148
Cuts at: 4912 5157 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 464 370 266 245 169
146 66
NaeI GCC'GGC
Cuts at: 2038 2813 2038
Size: 775 4896
NcoI C'CATG_G
Cuts at: 513 1065 2376 513
Size: 552 1311 3808
Fragments arranged by size:
3808 1311 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NgoMIV G'CCGG_C
Cuts at: 2036 2811 2036
Size: 775 4896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2800 3029 4574 1132
Size: 97 1571 229 1545 2229
Fragments arranged by size:
2229 1571 1545 229 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2332 2493 3023 830
Size: 1502 161 530 3478
Fragments arranged by size:
3478 1502 530 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2482 2482
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2328 2489 3019 4154 4835 5026 826
Size: 219 1283 161 530 1135 681 191 1471
Fragments arranged by size:
1471 1283 1135 681 530 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3775 4676 4938 820
Size: 197 926 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 2661 1517
Size: 1144 4527
StyI C'CwwG_G
Cuts at: 513 1065 2376 3110 513
Size: 552 1311 734 3074
Fragments arranged by size:
3074 1311 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3698 3715 3868 4053 4498 325
Size: 1255 2118 17 153 185 445 1498
Fragments arranged by size:
2118 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI