Restriction map for
pFN21AB8180
(Circular) (Six-base) MAPSORT of: pFN21AB8180.seq Check: 2525 from: 1 to: 5980
pFN21AB8180 5980 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3012 3323 1937
Size: 1075 311 4594
Fragments arranged by size:
4594 1075 311
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2880 3182 4043 5427 5695 1632
Size: 1248 302 861 1384 268 1917
Fragments arranged by size:
1917 1384 1248 861 302 268
AfeI AGC'GCT
Cuts at: 3058 3058
Size: 5980
AflII C'TTAA_G
Cuts at: 820 1017 2963 820
Size: 197 1946 3837
Fragments arranged by size:
3837 1946 197
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 3269 1166
Size: 2103 3877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2582 2585 2892 1184
Size: 1398 3 307 4272
Fragments arranged by size:
4272 1398 307 3
AlwNI CAG_nnn'CTG
Cuts at: 2818 3723 5295 2818
Size: 905 1572 3503
Fragments arranged by size:
3503 1572 905
ApaI G_GGCC'C
Cuts at: 2488 2845 2488
Size: 357 5623
ApaLI G'TGCA_C
Cuts at: 2877 4037 5193 2877
Size: 1160 1156 3664
Fragments arranged by size:
3664 1160 1156
ApoI r'AATT_y
Cuts at: 1493 3095 3289 3515 3667 5776 1493
Size: 1602 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 1602 226 194 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2838 3305 1870
Size: 73 895 467 4545
Fragments arranged by size:
4545 895 467 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2533 2750 3301 4756 618
Size: 325 217 627 746 217 551 1455 1842
Fragments arranged by size:
1842 1455 746 627 551 325 217 217
BanII G_rGCy'C
Cuts at: 1391 2013 2032 2488 2832 2845 3299 1391
Size: 622 19 456 344 13 454 4072
Fragments arranged by size:
4072 622 456 454 344 19 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2894 2914 928
Size: 971 995 20 3994
Fragments arranged by size:
3994 995 971 20
BbvCI CC'TCA_GC
Cuts at: 2014 2758 2014
Size: 744 5236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 2483 2855
Size: 107 122 71 631 605 493 318 372
Cuts at: 2855 3138 4591 136
Size: 283 1453 1525
Fragments arranged by size:
1525 1453 631 605 493 372 318 283
122 107 71
BglII A'GATC_T
Cuts at: 2643 5975 2643
Size: 3332 2648
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2488 2538 2845 2881 4041 5197 1790
Size: 698 50 307 36 1160 1156 2573
Fragments arranged by size:
2573 1160 1156 698 307 50 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2696 3711 4669
Size: 416 53 83 399 24 1279 1015 958
Cuts at: 4669 442
Size: 1753
Fragments arranged by size:
1753 1279 1015 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2582 1056
Size: 1526 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2828 4640 1239
Size: 822 767 1812 2579
Fragments arranged by size:
2579 1812 822 767
Bpu10I CC'TnA_GC
Cuts at: 2014 2574 2758 2014
Size: 560 184 5236
Fragments arranged by size:
5236 560 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3172 1186
Size: 1986 3994
BseYI C'CCAG_C
Cuts at: 2619 2797 2832 5183 5679 2619
Size: 178 35 2351 496 2920
Fragments arranged by size:
2920 2351 496 178 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2474 2881 3255 4041 4126 5197 2474
Size: 407 374 786 85 1071 3257
Fragments arranged by size:
3257 1071 786 407 374 85
BsmI GAATG_Cn'
Cuts at: 2561 3491 3584 2561
Size: 930 93 4957
Fragments arranged by size:
4957 930 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2032 2474 2488 2538 2832 2845
Size: 399 223 19 442 14 50 294 13
Cuts at: 2845 2881 3255 3299 4041 4126 5197 1391
Size: 36 374 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 374 294 223
85 50 44 36 19 14 13
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2946 3335 844
Size: 2102 389 3489
Fragments arranged by size:
3489 2102 389
BsrBI CCG'CTC
Cuts at: 1394 1823 3180 3867 1394
Size: 429 1357 687 3507
Fragments arranged by size:
3507 1357 687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2305 4624 4811 1700
Size: 110 495 2319 187 2869
Fragments arranged by size:
2869 2319 495 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3250 794
Size: 2456 3524
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 2317
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2643 2742 3310 3677 4062 4079 5531 5617
Size: 671 99 568 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 671 568 385 367 346 99
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2488 2700 513
Size: 552 939 484 212 3793
Fragments arranged by size:
3793 939 552 484 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2137 2407 487
Size: 683 56 911 270 4060
Fragments arranged by size:
4060 911 683 270 56
BtsI GCAGTG_nn'
Cuts at: 2409 2569 3496 4290 4310 2409
Size: 160 927 794 20 4079
Fragments arranged by size:
4079 927 794 160 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2307 2347
Size: 54 1425 50 275 18 381 96 40
Cuts at: 2347 2523 2697 3427 4316 5945 8
Size: 176 174 730 889 1629 43
Fragments arranged by size:
1629 1425 889 730 381 275 176 174
96 54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 3910 1353
Size: 1250 1307 3423
Fragments arranged by size:
3423 1307 1250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2236 2975 4567 4941 5087 387
Size: 1064 138 647 739 1592 374 146 1280
Fragments arranged by size:
1592 1280 1064 739 647 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2828 2880 3182 4043 4640 5427
Size: 393 429 767 52 302 861 597 787
Cuts at: 5427 5695 1239
Size: 268 1524
Fragments arranged by size:
1524 861 787 767 597 429 393 302
268 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2810 2360
Size: 450 5530
EcoO109I rG'GnC_Cy
Cuts at: 2041 2146 2803 2815 3146 3166 2041
Size: 105 657 12 331 20 4855
Fragments arranged by size:
4855 657 331 105 20 12
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2785 3060 5127 1678
Size: 1107 275 2067 2531
Fragments arranged by size:
2531 2067 1107 275
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3210 3242 4621 4653 4695
Size: 32 135 32 1638 32 1379 32 42
Cuts at: 4695 4727 1373
Size: 32 2626
Fragments arranged by size:
2626 1638 1379 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2263 3324 3570 669
Size: 988 281 325 1061 246 3079
Fragments arranged by size:
3079 1061 988 325 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 2699 10
Size: 54 1425 343 693 174 3291
Fragments arranged by size:
3291 1425 693 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2699 3269 3938 4297 4456 518
Size: 648 1365 168 570 669 359 159 2042
Fragments arranged by size:
2042 1365 669 648 570 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2436 2797 3396 4073 5221 5466 1606
Size: 830 361 599 677 1148 245 2120
Fragments arranged by size:
2120 1148 830 677 599 361 245
NaeI GCC'GGC
Cuts at: 2307 2307
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2004 2700 513
Size: 552 939 696 3793
Fragments arranged by size:
3793 939 696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 2305 2305
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 2578 1052
Size: 1526 4454
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3248 3338 4883 1132
Size: 97 2019 90 1545 2229
Fragments arranged by size:
2229 2019 1545 97 90
PasI CC'CwG_GG
Cuts at: 1348 2024 1348
Size: 676 5304
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 1108
Size: 1932 4048
PfoI T'CCnGG_A
Cuts at: 3099 3099
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2146 2803 3146 2041
Size: 105 657 343 4875
Fragments arranged by size:
4875 657 343 105
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspOMI G'GGCC_C
Cuts at: 2484 2841 2484
Size: 357 5623
PstI C_TGCA'G
Cuts at: 830 2548 3332 830
Size: 1718 784 3478
Fragments arranged by size:
3478 1718 784
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2797 2797
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SanDI GG'GwC_CC
Cuts at: 2041 3146 2041
Size: 1105 4875
SapI GCTCTTCn'nnn_
Cuts at: 2603 2603
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2378 2544 3328 4463 5144 5335 826
Size: 219 1333 166 784 1135 681 191 1471
Fragments arranged by size:
1471 1333 1135 784 681 219 191 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2840 3307 1872
Size: 968 467 4545
Fragments arranged by size:
4545 968 467
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2963 4084 4985 5247 820
Size: 197 926 1020 1121 901 262 1553
Fragments arranged by size:
1553 1121 1020 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2700 3419 513
Size: 552 939 696 719 3074
Fragments arranged by size:
3074 939 719 696 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2469 4007 4024 4177 4362 4807 325
Size: 1255 889 1538 17 153 185 445 1498
Fragments arranged by size:
1538 1498 1255 889 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2761 2902 2317
Size: 444 141 5395
Fragments arranged by size:
5395 444 141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 2838 3305 1870
Size: 968 467 4545
Fragments arranged by size:
4545 968 467
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI