Restriction map for
pFN21AB8179
(Circular) (Six-base) MAPSORT of: pFN21AB8179.seq Check: 4432 from: 1 to: 6007
pFN21AB8179 6007 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2898 2898
Size: 6007
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3164 3817 278
Size: 53 83 186 936 1628 653 2468
Fragments arranged by size:
2468 1628 936 653 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 3120 3328 3120
Size: 208 5799
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 3269 4070 5454 5722 1632
Size: 720 917 801 1384 268 1917
Fragments arranged by size:
1917 1384 917 801 720 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 2256 1166
Size: 1090 4917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 2227 2392 2485 2680 2907 3750 5322 2227
Size: 165 93 195 227 843 1572 2912
Fragments arranged by size:
2912 1572 843 227 195 165 93
ApaLI G'TGCA_C
Cuts at: 3055 4064 5220 3055
Size: 1009 1156 3842
Fragments arranged by size:
3842 1156 1009
ApoI r'AATT_y
Cuts at: 1493 3316 3542 3694 5803 1493
Size: 1823 226 152 2109 1697
Fragments arranged by size:
2109 1823 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3234 4561 160
Size: 872 2202 1327 1606
Fragments arranged by size:
2202 1606 1327 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 2934 3210 3332 1870
Size: 73 322 669 276 122 4545
Fragments arranged by size:
4545 669 322 276 122 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2495 2528 2495
Size: 33 5974
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3120 3328 4783 618
Size: 325 217 627 1333 208 1455 1842
Fragments arranged by size:
1842 1455 1333 627 325 217 208
BanII G_rGCy'C
Cuts at: 1391 3326 1391
Size: 1935 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5036
BbvCI CC'TCA_GC
Cuts at: 2774 2774
Size: 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3286 3320 4198 4232 1903
Size: 34 1349 34 878 34 3678
Fragments arranged by size:
3678 1349 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 3899 5109 2272
Size: 1627 1210 3170
Fragments arranged by size:
3170 1627 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2427 2916 6002 2427
Size: 489 3086 2432
Fragments arranged by size:
3086 2432 489
BlpI GC'TnA_GC
Cuts at: 2127 2528 3424 2127
Size: 401 896 4710
Fragments arranged by size:
4710 896 401
Bme1580I G_kGCm'C
Cuts at: 1790 2378 3059 4068 5224 1790
Size: 588 681 1009 1156 2573
Fragments arranged by size:
2573 1156 1009 681 588
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2917 3069 3738
Size: 416 53 83 399 24 1500 152 669
Cuts at: 3738 4696 442
Size: 958 1753
Fragments arranged by size:
1753 1500 958 669 416 399 152 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3236
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2132 2292 2723 2786 4667 1239
Size: 872 21 160 431 63 1881 2579
Fragments arranged by size:
2579 1881 872 431 160 63 21
Bpu10I CC'TnA_GC
Cuts at: 2774 2978 2774
Size: 204 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2918 4670 882
Size: 617 1419 1752 2219
Fragments arranged by size:
2219 1752 1419 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 2298 3798 493
Size: 711 906 188 1500 2702
Fragments arranged by size:
2702 1500 906 711 188
BsaBI GATnn'nnATC
Cuts at: 2495 3026 6001 2495
Size: 531 2975 2501
Fragments arranged by size:
2975 2501 531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2940 3161 3814 4196
Size: 53 83 186 936 1407 221 653 382
Cuts at: 4196 4831 275
Size: 635 1451
Fragments arranged by size:
1451 1407 936 653 635 382 221 186
83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5782
Fragments arranged by size:
5782 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2390 2555 1186
Size: 922 282 165 4638
Fragments arranged by size:
4638 922 282 165
BseYI C'CCAG_C
Cuts at: 2336 2860 2953 5210 5706 2336
Size: 524 93 2257 496 2637
Fragments arranged by size:
2637 2257 524 496 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2240 2609 2699 2708 2795 1163
Size: 573 351 153 369 90 9 87 4375
Fragments arranged by size:
4375 573 369 351 153 90 87 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 2328 3059 4068 4153 5224 2297
Size: 31 731 1009 85 1071 3080
Fragments arranged by size:
3080 1071 1009 731 85 31
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
BsmBI CGTCTCn'nnnn_
Cuts at: 2668 2668
Size: 6007
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2328 2378 3059 3326 4068 4153
Size: 399 507 31 50 681 267 742 85
Cuts at: 4153 5224 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 681 507 399 267 85
50 31
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2862 2898 3362 844
Size: 2018 36 464 3489
Fragments arranged by size:
3489 2018 464 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 3894 1394
Size: 429 260 1811 3507
Fragments arranged by size:
3507 1811 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2302 4651 4838 1700
Size: 110 492 2349 187 2869
Fragments arranged by size:
2869 2349 492 187 110
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 3760
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2485 2524 2680 2722 3114 794
Size: 1691 39 156 42 392 3687
Fragments arranged by size:
3687 1691 392 156 42 39
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2427 2481 2823 2916 3337 3704 4089 4106
Size: 455 54 342 93 421 367 385 17
Cuts at: 4106 5558 5644 5656 6002 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 455 421 385 367 346 342
93 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3267 513
Size: 552 939 1263 3253
Fragments arranged by size:
3253 1263 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 2018 2263 3059 3523 4317 4337 2018
Size: 245 796 464 794 20 3688
Fragments arranged by size:
3688 796 794 464 245 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DraIII CAC_nnn'GTG
Cuts at: 2110 3130 2110
Size: 1020 4987
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2300 2493
Size: 54 1425 50 275 18 303 167 193
Cuts at: 2493 2535 3196 3454 4343 5972 8
Size: 42 661 258 889 1629 43
Fragments arranged by size:
1629 1425 889 661 303 275 258 193
167 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3937 1353
Size: 2584 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 3140 4594 4968 5114 387
Size: 1064 138 1134 417 1454 374 146 1280
Fragments arranged by size:
1454 1280 1134 1064 417 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2132 2292 2352 2723 2786 3269
Size: 393 479 21 160 60 371 63 483
Cuts at: 3269 4070 4667 5454 5722 1239
Size: 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 483 479 393 371
268 160 63 60 21
EcoO109I rG'GnC_Cy
Cuts at: 2044 2154 2389 2642 2750 2770 2044
Size: 110 235 253 108 20 5281
Fragments arranged by size:
5281 253 235 110 108 20
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2680 3011 5154 1678
Size: 1002 331 2143 2531
Fragments arranged by size:
2531 2143 1002 331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373
Size: 32 135 32 3076 32 42 32 2626
Fragments arranged by size:
3076 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3124 3332 3124
Size: 208 5799
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 3195 5121 5305 1305
Size: 1326 564 1926 184 2007
Fragments arranged by size:
2007 1926 1326 564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2495 3198 10
Size: 54 1425 343 303 360 703 2819
Fragments arranged by size:
2819 1425 703 360 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 3965 4324 4483 518
Size: 648 1090 1709 359 159 2042
Fragments arranged by size:
2042 1709 1090 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2764 2818 3049 3423 4100 5248 5493
Size: 513 645 54 231 374 677 1148 245
Cuts at: 5493 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 645 513 374 245 231
54
NaeI GCC'GGC
Cuts at: 2304 2304
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 2004 3267 513
Size: 552 939 1263 3253
Fragments arranged by size:
3253 1263 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NgoMIV G'CCGG_C
Cuts at: 2302 2302
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2527 3365 4910 1132
Size: 97 1298 838 1545 2229
Fragments arranged by size:
2229 1545 1298 838 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2893 1108
Size: 1785 4222
PfoI T'CCnGG_A
Cuts at: 2613 2613
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PmlI CAC'GTG
Cuts at: 2298 2298
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2437 4076 4108 5589 5621 2405
Size: 32 1639 32 1481 32 2791
Fragments arranged by size:
2791 1639 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2154 2642 2044
Size: 110 488 5409
Fragments arranged by size:
5409 488 110
PshAI GACnn'nnGTC
Cuts at: 1613 2640 1613
Size: 1027 4980
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 2052 2490 3359 830
Size: 1222 438 869 3478
Fragments arranged by size:
3478 1222 869 438
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2119 2764 2119
Size: 645 5362
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SanDI GG'GwC_CC
Cuts at: 2154 2154
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2048 2486 3355 4490 5171 5362 826
Size: 219 1003 438 869 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 869 681 438 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 2936 3334 1872
Size: 1064 398 4545
Fragments arranged by size:
4545 1064 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 2527 3365 1132
Size: 1395 838 3774
Fragments arranged by size:
3774 1395 838
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6007
StyI C'CwwG_G
Cuts at: 513 1065 2004 3267 3446 513
Size: 552 939 1263 179 3074
Fragments arranged by size:
3074 1263 939 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4034 4051 4204 4389 4834 325
Size: 1255 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 4253 5965
Size: 274 80 33 51 1443 366 1910 1712
Cuts at: 5965 96
Size: 138
Fragments arranged by size:
1910 1712 1443 366 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 2042 1316
Size: 726 5281
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2316 2176
Size: 140 5867
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 2934 3332 1870
Size: 1064 398 4545
Fragments arranged by size:
4545 1064 398
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3162 3815 276
Size: 53 83 186 936 1628 653 2468
Fragments arranged by size:
2468 1628 936 653 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI