Restriction map for
pFN21AB8177
(Circular) (Six-base) MAPSORT of: pFN21AB8177.seq Check: 9413 from: 1 to: 6307
pFN21AB8177 6307 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2539 2539
Size: 6307
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3382 3650 1937
Size: 1445 268 4594
Fragments arranged by size:
4594 1445 268
Acc65I G'GTAC_C
Cuts at: 2491 3628 2491
Size: 1137 5170
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 2869 4370 5754 6022 1632
Size: 819 418 1501 1384 268 1917
Fragments arranged by size:
1917 1501 1384 819 418 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 2562 5206 6183 1203
Size: 1359 2644 977 1327
Fragments arranged by size:
2644 1359 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 2211 2447 2742 1166
Size: 1045 236 295 4731
Fragments arranged by size:
4731 1045 295 236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6307
AlwNI CAG_nnn'CTG
Cuts at: 2516 3485 4050 5622 2516
Size: 969 565 1572 3201
Fragments arranged by size:
3201 1572 969 565
ApaLI G'TGCA_C
Cuts at: 2204 3589 4364 5520 2204
Size: 1385 775 1156 2991
Fragments arranged by size:
2991 1385 1156 775
ApoI r'AATT_y
Cuts at: 1493 2506 3208 3228 3616 3842 3994 6103 1493
Size: 1013 702 20 388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 702 388 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 2550 4861 160
Size: 872 1518 2311 1606
Fragments arranged by size:
2311 1606 1518 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 3632 1870
Size: 73 1689 4545
Fragments arranged by size:
4545 1689 73
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 3628 5083 618
Size: 325 217 627 704 1137 1455 1842
Fragments arranged by size:
1842 1455 1137 704 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2643 2685 3626 1391
Size: 1252 42 941 4072
Fragments arranged by size:
4072 1252 941 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 3153 928
Size: 971 963 291 4082
Fragments arranged by size:
4082 971 963 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 1903
Size: 34 2561 34 3678
Fragments arranged by size:
3678 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 2929 3474 1548
Size: 1381 545 4381
Fragments arranged by size:
4381 1381 545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4918 136
Size: 107 122 71 631 605 3246 1525
Fragments arranged by size:
3246 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2880 6302 2880
Size: 3422 2885
BlpI GC'TnA_GC
Cuts at: 3724 3724
Size: 6307
Bme1580I G_kGCm'C
Cuts at: 1790 2208 3593 4368 5524 1790
Size: 418 1385 775 1156 2573
Fragments arranged by size:
2573 1385 1156 775 418
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2230 4038 4996
Size: 416 53 83 399 24 813 1808 958
Cuts at: 4996 442
Size: 1753
Fragments arranged by size:
1808 1753 958 813 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 3074 3206 3593 4967 1239
Size: 1092 743 132 387 1374 2579
Fragments arranged by size:
2579 1374 1092 743 387 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1995 4432 5297 5595 1995
Size: 2437 865 298 2707
Fragments arranged by size:
2707 2437 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2390 2647 3087 3497 4970 882
Size: 617 891 257 440 410 1473 2219
Fragments arranged by size:
2219 1473 891 617 440 410 257
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 2934 6301 2934
Size: 3367 2940
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4114 4496 5131 275
Size: 53 83 186 936 2581 382 635 1451
Fragments arranged by size:
2581 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4740 5138 5412 5559 5970 4740
Size: 398 274 147 411 5077
Fragments arranged by size:
5077 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2049 2079 2321 2351 1178
Size: 30 165 30 646 30 242 30 5134
Fragments arranged by size:
5134 646 242 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2623 3482 1186
Size: 1156 281 859 4011
Fragments arranged by size:
4011 1156 859 281
BseYI C'CCAG_C
Cuts at: 2633 2859 5510 6006 2633
Size: 226 2651 496 2934
Fragments arranged by size:
2934 2651 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 3459 1163
Size: 573 524 1199 4011
Fragments arranged by size:
4011 1199 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2851 3593 4368 4453 5524 2208
Size: 643 742 775 85 1071 2991
Fragments arranged by size:
2991 1071 775 742 643 85
BsmI GAATG_Cn'
Cuts at: 2262 3818 3911 2262
Size: 1556 93 4658
Fragments arranged by size:
4658 1556 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2643 2685 2851 3593 3626 4368
Size: 399 418 435 42 166 742 33 742
Cuts at: 4368 4453 5524 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 742 435 418 399 166
85 42 33
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2539 3662 844
Size: 1695 1123 3489
Fragments arranged by size:
3489 1695 1123
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3162 3414 4802 4976 65
Size: 1183 1914 252 1388 174 1396
Fragments arranged by size:
1914 1396 1388 1183 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 2385 4951 5138 1700
Size: 110 286 289 2566 187 2869
Fragments arranged by size:
2869 2566 289 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 2473 96
Size: 2202 175 3930
Fragments arranged by size:
3930 2202 175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 4367 5379 4367
Size: 1012 5295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6307
BstBI TT'CG_AA
Cuts at: 5111 5111
Size: 6307
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 2880 2888 3543 3637 4004 4389 4406 5858
Size: 908 8 655 94 367 385 17 1452
Cuts at: 5858 5944 5956 6302 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 908 655 385 367 346 94
86 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2400 2418 513
Size: 552 1335 18 4402
Fragments arranged by size:
4402 1335 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5568
Fragments arranged by size:
5568 683 56
BtsI GCAGTG_nn'
Cuts at: 3823 4617 4637 3823
Size: 794 20 5493
Fragments arranged by size:
5493 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3754 4643 6272
Size: 54 1425 50 275 18 1924 889 1629
Cuts at: 6272 8
Size: 43
Fragments arranged by size:
1924 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3593 4237 1353
Size: 2240 644 3423
Fragments arranged by size:
3423 2240 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4894 5268 5414 387
Size: 1064 138 3305 374 146 1280
Fragments arranged by size:
3305 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2451 2869 3074 3206 3593 4370
Size: 393 699 120 418 205 132 387 777
Cuts at: 4370 4967 5754 6022 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 777 699 597 418 393 387
268 205 132 120
EcoNI CCTnn'n_nnAGG
Cuts at: 3020 3020
Size: 6307
EcoO109I rG'GnC_Cy
Cuts at: 3261 3261
Size: 6307
EcoRI G'AATT_C
Cuts at: 2506 3616 3994 2506
Size: 1110 378 4819
Fragments arranged by size:
4819 1110 378
EcoRV GAT'ATC
Cuts at: 2267 2267
Size: 6307
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 5454 1678
Size: 3776 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3466 3498 4948 4980 5022
Size: 32 135 32 1894 32 1450 32 42
Cuts at: 5022 5054 1373
Size: 32 2626
Fragments arranged by size:
2626 1894 1450 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3523 3651 3897 669
Size: 988 281 1585 128 246 3079
Fragments arranged by size:
3079 1585 988 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6307
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KpnI G_GTAC'C
Cuts at: 2495 3632 2495
Size: 1137 5170
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2203 2953 3510 5421 5605 1305
Size: 898 750 557 1911 184 2007
Fragments arranged by size:
2007 1911 898 750 557 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4485
Fragments arranged by size:
4485 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2447 2742 4265 4624 4783 518
Size: 648 1045 236 295 1523 359 159 2042
Fragments arranged by size:
2042 1523 1045 648 359 295 236 159
MspA1I CmG'CkG
Cuts at: 1606 2363 3296 3723 4400 5548 5793 1606
Size: 757 933 427 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 757 677 427 245
NcoI C'CATG_G
Cuts at: 513 1065 2400 2418 513
Size: 552 1335 18 4402
Fragments arranged by size:
4402 1335 552 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 2566 3665 5210 1132
Size: 97 1337 1099 1545 2229
Fragments arranged by size:
2229 1545 1337 1099 97
PasI CC'CwG_GG
Cuts at: 1348 2516 1348
Size: 1168 5139
PciI A'CATG_T
Cuts at: 2562 5206 2562
Size: 2644 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2696 1108
Size: 1588 4719
PfoI T'CCnGG_A
Cuts at: 3222 3257 3222
Size: 35 6272
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3261 3261
Size: 6307
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PstI C_TGCA'G
Cuts at: 830 3659 830
Size: 2829 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2363 3296 2363
Size: 933 5374
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SexAI A'CCwGG_T
Cuts at: 2187 2568 2187
Size: 381 5926
SfcI C'TryA_G
Cuts at: 826 1045 2667 3655 4790 5471 5662 826
Size: 219 1622 988 1135 681 191 1471
Fragments arranged by size:
1622 1471 1135 988 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 3634 1872
Size: 1762 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 4411 5312 5574 820
Size: 197 926 67 2401 901 262 1553
Fragments arranged by size:
2401 1553 926 901 262 197 67
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 3665 1132
Size: 2533 3774
SspI AAT'ATT
Cuts at: 5 52 3099 4231 5
Size: 47 3047 1132 2081
Fragments arranged by size:
3047 2081 1132 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6307
StyI C'CwwG_G
Cuts at: 513 1065 2400 2418 2643 3746 513
Size: 552 1335 18 225 1103 3074
Fragments arranged by size:
3074 1335 1103 552 225 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 4334 4351 4504 4689 5134 325
Size: 1255 1293 1461 17 153 185 445 1498
Fragments arranged by size:
1498 1461 1293 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 2473 4553
Size: 274 80 33 51 1443 321 175 2080
Cuts at: 4553 6265 96
Size: 1712 138
Fragments arranged by size:
2080 1712 1443 321 274 175 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6267 6299 6267
Size: 32 6275
Tth111I GACn'n_nGTC
Cuts at: 1316 2356 1316
Size: 1040 5267
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3049 3568 3049
Size: 519 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 3632 1870
Size: 1762 4545
XmnI GAAnn'nnTTC
Cuts at: 4436 4436
Size: 6307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI