Restriction map for
pFN21AB8169
(Circular) (Six-base) MAPSORT of: pFN21AB8169.seq Check: 9734 from: 1 to: 5701
pFN21AB8169 5701 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2056 2056
Size: 5701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 3044 1937
Size: 922 185 4594
Fragments arranged by size:
4594 922 185
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 2593 4430 2593
Size: 1837 3864
AleI CACnn'nnGTG
Cuts at: 1166 2511 1166
Size: 1345 4356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2426 2458 2426
Size: 32 5669
AlwNI CAG_nnn'CTG
Cuts at: 2122 3444 5016 2122
Size: 1322 1572 2807
Fragments arranged by size:
2807 1572 1322
ApaI G_GGCC'C
Cuts at: 2196 2916 2196
Size: 720 4981
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 2030 3010 3236 3388 5497 1493
Size: 537 980 226 152 2109 1697
Fragments arranged by size:
2109 1697 980 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2491 3026 1870
Size: 73 548 535 4545
Fragments arranged by size:
4545 548 535 73
BamHI G'GATC_C
Cuts at: 2303 2730 3031 3398 2303
Size: 427 301 367 4606
Fragments arranged by size:
4606 427 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2946 3022 4477 618
Size: 325 217 627 1159 76 1455 1842
Fragments arranged by size:
1842 1455 1159 627 325 217 76
BanII G_rGCy'C
Cuts at: 1391 2196 2280 2325 2561 2916 3020 1391
Size: 805 84 45 236 355 104 4072
Fragments arranged by size:
4072 805 355 236 104 84 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2297 928
Size: 971 398 4332
Fragments arranged by size:
4332 971 398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3892 3926 1903
Size: 34 50 34 1871 34 3678
Fragments arranged by size:
3678 1871 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2014 2284 2883
Size: 107 122 71 631 605 342 270 599
Cuts at: 2883 4312 136
Size: 1429 1525
Fragments arranged by size:
1525 1429 631 605 599 342 270 122
107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 2643 3118 2643
Size: 475 5226
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2879 2916 2951 3762 4918 1790
Size: 406 683 37 35 811 1156 2573
Fragments arranged by size:
2573 1156 811 683 406 37 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2561 2959 3432
Size: 416 53 83 399 24 1144 398 473
Cuts at: 3432 4390 442
Size: 958 1753
Fragments arranged by size:
1753 1144 958 473 416 399 398 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2428 2497 4361 1239
Size: 875 314 69 1864 2579
Fragments arranged by size:
2579 1864 875 314 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2050 4364 882
Size: 617 551 2314 2219
Fragments arranged by size:
2314 2219 617 551
BsaAI yAC'GTr
Cuts at: 493 1204 2794 3492 493
Size: 711 1590 698 2702
Fragments arranged by size:
2702 1590 711 698
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3508 3890 4525
Size: 53 83 186 936 834 1141 382 635
Cuts at: 4525 275
Size: 1451
Fragments arranged by size:
1451 1141 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 1178
Size: 30 165 30 1023 30 4423
Fragments arranged by size:
4423 1023 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2990 1186
Size: 1804 3897
BseYI C'CCAG_C
Cuts at: 2279 2384 2726 4904 5400 2279
Size: 105 342 2178 496 2580
Fragments arranged by size:
2580 2178 496 342 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2489 1163
Size: 573 522 231 4375
Fragments arranged by size:
4375 573 522 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2561 3762 3847 4918 2325
Size: 236 1201 85 1071 3108
Fragments arranged by size:
3108 1201 1071 236 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
BsmBI CGTCTCn'nnnn_
Cuts at: 2358 2358
Size: 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2280 2325 2561 2879 2916 2951
Size: 399 406 84 45 236 318 37 35
Cuts at: 2951 3020 3762 3847 4918 1391
Size: 69 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 318 236 85
84 69 45 37 35
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 2895 3590 5320 2895
Size: 695 1730 3276
Fragments arranged by size:
3276 1730 695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 2257 3056 844
Size: 1212 201 799 3489
Fragments arranged by size:
3489 1212 799 201
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4196 4370 65
Size: 1183 2948 174 1396
Fragments arranged by size:
2948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4345 4532 1700
Size: 110 2535 187 2869
Fragments arranged by size:
2869 2535 187 110
BsrGI T'GTAC_A
Cuts at: 96 2718 96
Size: 2622 3079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2381 794
Size: 1587 4114
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 2147 2147
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 2303 2730 3031 3398 3783 3800 5252 5338
Size: 331 427 301 367 385 17 1452 86
Cuts at: 5338 5350 5696 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 427 385 367 346 331 301
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2146 513
Size: 552 1081 4068
Fragments arranged by size:
4068 1081 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 2960 2993 3217 4011 4031 2660
Size: 300 33 224 794 20 4330
Fragments arranged by size:
4330 794 300 224 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DraIII CAC_nnn'GTG
Cuts at: 2797 2943 2797
Size: 146 5555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2141 4708 809
Size: 507 825 2567 1802
Fragments arranged by size:
2567 1802 825 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 3148 4037
Size: 54 1425 50 275 18 1054 264 889
Cuts at: 4037 5666 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1054 889 275 264 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2566 3631 1353
Size: 1213 1065 3423
Fragments arranged by size:
3423 1213 1065
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 2559 2323
Size: 236 5465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2428 2497 3764 4361 5148 5416
Size: 393 482 314 69 1267 597 787 268
Cuts at: 5416 1239
Size: 1524
Fragments arranged by size:
1524 1267 787 597 482 393 314 268
69
EcoO109I rG'GnC_Cy
Cuts at: 2192 2193 2482 2779 2192
Size: 1 289 297 5114
Fragments arranged by size:
5114 297 289 1
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 4848 1678
Size: 3170 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 2579 2611 2715
Size: 32 135 32 719 32 256 32 104
Cuts at: 2715 2747 4342 4374 4416 4448 1373
Size: 32 1595 32 42 32 2626
Fragments arranged by size:
2626 1595 719 256 135 104 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2886 10
Size: 54 1425 343 1054 2825
Fragments arranged by size:
2825 1425 1054 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 3659 4018 4177 518
Size: 648 1345 1148 359 159 2042
Fragments arranged by size:
2042 1345 1148 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2283 3117 3794 4942 5187 1606
Size: 677 834 677 1148 245 2120
Fragments arranged by size:
2120 1148 834 677 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2146 513
Size: 552 1081 4068
Fragments arranged by size:
4068 1081 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 3059 4604 1132
Size: 97 1830 1545 2229
Fragments arranged by size:
2229 1830 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2332 1348
Size: 984 4717
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PfoI T'CCnGG_A
Cuts at: 2130 2130
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PmlI CAC'GTG
Cuts at: 2794 2794
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 3770 3802 5283 5315 2426
Size: 32 1312 32 1481 32 2812
Fragments arranged by size:
2812 1481 1312 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 2779 2482
Size: 297 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 2538 3271 2538
Size: 733 4968
PspOMI G'GGCC_C
Cuts at: 2192 2912 2192
Size: 720 4981
PstI C_TGCA'G
Cuts at: 830 2118 2320 2377 2823 3053 830
Size: 1288 202 57 446 230 3478
Fragments arranged by size:
3478 1288 446 230 202 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SacI G_AGCT'C
Cuts at: 2325 2561 2325
Size: 236 5465
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SanDI GG'GwC_CC
Cuts at: 2482 2482
Size: 5701
SapI GCTCTTCn'nnn_
Cuts at: 2566 2566
Size: 5701
SbfI CC_TGCA'GG
Cuts at: 2377 3053 2377
Size: 676 5025
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SexAI A'CCwGG_T
Cuts at: 2889 2939 2889
Size: 50 5651
SfcI C'TryA_G
Cuts at: 826 1045 2099 2114 2316 2373 2819 3049 4184
Size: 219 1054 15 202 57 446 230 1135
Cuts at: 4184 4865 5056 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1054 681 446 230 219 202
191 57 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 2146 2833 3140 513
Size: 552 1081 687 307 3074
Fragments arranged by size:
3074 1081 687 552 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 2419 2593 3728 3745 3898 4083
Size: 1255 573 266 174 1135 17 153 185
Cuts at: 4083 4528 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1135 573 445 266 185 174
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2718 2807 3947
Size: 274 80 33 51 1443 741 89 1140
Cuts at: 3947 5659 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1140 741 274 138 89 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 2176 3037 2176
Size: 861 4840
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 2159 2438 3830 2159
Size: 279 1392 4030
Fragments arranged by size:
4030 1392 279
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI
SwaI XcmI