Restriction map for
pFN21AB8167
(Circular) (Six-base) MAPSORT of: pFN21AB8167.seq Check: 5947 from: 1 to: 6022
pFN21AB8167 6022 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2048 3069 3365 1937
Size: 111 1021 296 4594
Fragments arranged by size:
4594 1021 296 111
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 4085 5469 5737 1632
Size: 723 1730 1384 268 1917
Fragments arranged by size:
1917 1730 1384 723 268
AfeI AGC'GCT
Cuts at: 2020 2892 3088 2020
Size: 872 196 4954
Fragments arranged by size:
4954 872 196
AflII C'TTAA_G
Cuts at: 820 1017 2600 820
Size: 197 1583 4242
Fragments arranged by size:
4242 1583 197
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 3036 1166
Size: 1870 4152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 5990
AlwNI CAG_nnn'CTG
Cuts at: 2222 2552 3765 5337 2222
Size: 330 1213 1572 2907
Fragments arranged by size:
2907 1572 1213 330
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 2180 3331 3557 3709 5818 1493
Size: 687 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 687 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 3347 1870
Size: 73 1404 4545
Fragments arranged by size:
4545 1404 73
BamHI G'GATC_C
Cuts at: 3352 3719 3352
Size: 367 5655
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3038 3343 4798 618
Size: 325 217 627 1251 305 1455 1842
Fragments arranged by size:
1842 1455 1251 627 325 305 217
BanII G_rGCy'C
Cuts at: 1391 3341 1391
Size: 1950 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 2602 928
Size: 971 501 202 4348
Fragments arranged by size:
4348 971 501 202
BbvCI CC'TCA_GC
Cuts at: 2703 2703
Size: 6022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 3439 3439
Size: 6022
Bme1580I G_kGCm'C
Cuts at: 1790 3238 4083 5239 1790
Size: 1448 845 1156 2573
Fragments arranged by size:
2573 1448 1156 845
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3753 4711
Size: 416 53 83 399 24 643 1693 958
Cuts at: 4711 442
Size: 1753
Fragments arranged by size:
1753 1693 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2325 2371 2325
Size: 46 5976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2550 2699 3119 4682 1239
Size: 1311 149 420 1563 2579
Fragments arranged by size:
2579 1563 1311 420 149
Bpu10I CC'TnA_GC
Cuts at: 2703 3160 2703
Size: 457 5565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2915 4147 5012 5310 2711
Size: 204 1232 865 298 3423
Fragments arranged by size:
3423 1232 865 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4685 882
Size: 617 3186 2219
Fragments arranged by size:
3186 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4211 4846 275
Size: 53 83 186 936 2296 382 635 1451
Fragments arranged by size:
2296 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2126 4455 4853 5127 5274 5685 2126
Size: 2329 398 274 147 411 2463
Fragments arranged by size:
2463 2329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5797
Fragments arranged by size:
5797 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2249 2426 2429 2549 2837 2962 1186
Size: 997 66 177 3 120 288 125 4246
Fragments arranged by size:
4246 997 288 177 125 120 66 3
BseYI C'CCAG_C
Cuts at: 2054 3269 5225 5721 2054
Size: 1215 1956 496 2355
Fragments arranged by size:
2355 1956 1215 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3472 4233 5261 1540
Size: 275 186 1471 761 1028 2301
Fragments arranged by size:
2301 1471 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2473 4083 4168 5239 2473
Size: 1610 85 1071 3256
Fragments arranged by size:
3256 1610 1071 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 3238 3341 4083 4168 5239 1391
Size: 399 683 765 103 742 85 1071 2174
Fragments arranged by size:
2174 1071 765 742 683 399 103 85
BspEI T'CCGG_A
Cuts at: 2126 5685 2126
Size: 3559 2463
BspHI T'CATG_A
Cuts at: 2025 3911 5641 2025
Size: 1886 1730 2406
Fragments arranged by size:
2406 1886 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3130 3377 844
Size: 2286 247 3489
Fragments arranged by size:
3489 2286 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2443 3292 3909 1394
Size: 429 620 849 617 3507
Fragments arranged by size:
3507 849 620 617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2080 4517 4691 65
Size: 1183 832 2437 174 1396
Fragments arranged by size:
2437 1396 1183 832 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4666 4853 1700
Size: 110 2856 187 2869
Fragments arranged by size:
2869 2856 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 4082 5094 4082
Size: 1012 5010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2792 794
Size: 1998 4024
BstBI TT'CG_AA
Cuts at: 2617 4826 2617
Size: 2209 3813
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 3352 3719 4104 4121 5573 5659 5671 6017
Size: 1380 367 385 17 1452 86 12 346
Cuts at: 6017 1972
Size: 1977
Fragments arranged by size:
1977 1452 1380 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3070 3070
Size: 6022
Bsu36I CC'TnA_GG
Cuts at: 2223 2223
Size: 6022
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5283
Fragments arranged by size:
5283 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 3538 4332 4352 2407
Size: 1131 794 20 4077
Fragments arranged by size:
4077 1131 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3190 3225 529
Size: 35 2626 35 3326
Fragments arranged by size:
3326 2626 35 35
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DraIII CAC_nnn'GTG
Cuts at: 3187 3187
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3162 5029 809
Size: 507 1846 1867 1802
Fragments arranged by size:
1867 1846 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3293 3469 4358
Size: 54 1425 50 275 18 1463 176 889
Cuts at: 4358 5987 8
Size: 1629 43
Fragments arranged by size:
1629 1463 1425 889 275 176 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2292 2403 2484 2556 2733 2757 2937 3952
Size: 939 111 81 72 177 24 180 1015
Cuts at: 3952 1353
Size: 3423
Fragments arranged by size:
3423 1015 939 180 177 111 81 72
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2550 2699 3119 4085 4682 5469
Size: 393 723 195 149 420 966 597 787
Cuts at: 5469 5737 1239
Size: 268 1524
Fragments arranged by size:
1524 966 787 723 597 420 393 268
195 149
EcoNI CCTnn'n_nnAGG
Cuts at: 2936 2936
Size: 6022
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 6022
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 2022 2894 3090 5169 1678
Size: 344 872 196 2079 2531
Fragments arranged by size:
2531 2079 872 344 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2528 2560 2578
Size: 32 135 32 656 32 268 32 18
Cuts at: 2578 2610 3188 3220 3281 3313 4663 4695 4737
Size: 32 578 32 61 32 1350 32 42
Cuts at: 4737 4769 1373
Size: 32 2626
Fragments arranged by size:
2626 1350 656 578 268 135 61 42
32 32 32 32 32 32 32 32
32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5136 5320 1305
Size: 3831 184 2007
Fragments arranged by size:
3831 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4200
Fragments arranged by size:
4200 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 3036 3980 4339 4498 518
Size: 648 1341 529 944 359 159 2042
Fragments arranged by size:
2042 1341 944 648 529 359 159
MspA1I CmG'CkG
Cuts at: 1606 3269 3438 4115 5263 5508 1606
Size: 1663 169 677 1148 245 2120
Fragments arranged by size:
2120 1663 1148 677 245 169
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5470
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 2926 3380 4925 1132
Size: 97 1697 454 1545 2229
Fragments arranged by size:
2229 1697 1545 454 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6022
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2622 2654 4091 4123 5604 5636 2622
Size: 32 1437 32 1481 32 3008
Fragments arranged by size:
3008 1481 1437 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PstI C_TGCA'G
Cuts at: 830 3374 830
Size: 2544 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
PvuII CAG'CTG
Cuts at: 3269 3269
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 3370 4505 5186 5377 826
Size: 219 2325 1135 681 191 1471
Fragments arranged by size:
2325 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 3349 1872
Size: 1477 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 2726 2894 4126 5027 5289
Size: 197 926 657 126 168 1232 901 262
Cuts at: 5289 820
Size: 1553
Fragments arranged by size:
1553 1232 926 901 657 262 197 168
126
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 2926 3380 1132
Size: 1794 454 3774
Fragments arranged by size:
3774 1794 454
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 3461 513
Size: 552 2396 3074
Fragments arranged by size:
3074 2396 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1999 2643 4049 4066 4219 4404 4849
Size: 1255 419 644 1406 17 153 185 445
Cuts at: 4849 325
Size: 1498
Fragments arranged by size:
1498 1406 1255 644 445 419 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2565 2718 4268
Size: 274 80 33 51 1443 588 153 1550
Cuts at: 4268 5980 96
Size: 1712 138
Fragments arranged by size:
1712 1550 1443 588 274 153 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5982 6014 5982
Size: 32 5990
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 2755
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 3347 1870
Size: 1477 4545
XmnI GAAnn'nnTTC
Cuts at: 2181 4151 2181
Size: 1970 4052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI EcoICRI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI