Restriction map for
pFN21AB8162
(Circular) (Six-base) MAPSORT of: pFN21AB8162.seq Check: 535 from: 1 to: 5782
pFN21AB8162 5782 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2841 3845 5229 5497 1632
Size: 1209 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1209 1004 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2245 4681 5658 1203
Size: 1042 2436 977 1327
Fragments arranged by size:
2436 1327 1042 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2777 1166
Size: 1611 4171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2441 1184
Size: 1257 4525
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 2123 3839 4995 2123
Size: 1716 1156 2910
Fragments arranged by size:
2910 1716 1156
ApoI r'AATT_y
Cuts at: 1493 3013 3091 3317 3469 5578 1493
Size: 1520 78 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152 78
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 3107 1870
Size: 73 91 1073 4545
Fragments arranged by size:
4545 1073 91 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2918 3067 3101 1391
Size: 1527 149 34 4072
Fragments arranged by size:
4072 1527 149 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 928
Size: 971 240 4571
Fragments arranged by size:
4571 971 240
BbvCI CC'TCA_GC
Cuts at: 2148 2148
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2039 2073 3973 4007 1903
Size: 34 102 34 1900 34 3678
Fragments arranged by size:
3678 1900 102 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BfrBI ATG'CAT
Cuts at: 2428 2645 2428
Size: 217 5565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2514 2531 3843 4999 1790
Size: 337 387 17 1312 1156 2573
Fragments arranged by size:
2573 1312 1156 387 337 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2525 3513 4471
Size: 416 53 83 399 24 1108 988 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1108 988 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4442 1239
Size: 3203 2579
Bpu10I CC'TnA_GC
Cuts at: 2148 2148
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2745 3907 4772 5070 2745
Size: 1162 865 298 3457
Fragments arranged by size:
3457 1162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2469 4445 882
Size: 617 970 1976 2219
Fragments arranged by size:
2219 1976 970 617
BsaAI yAC'GTr
Cuts at: 493 1204 2248 3573 493
Size: 711 1044 1325 2702
Fragments arranged by size:
2702 1325 1044 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2311 4215 4613 4887 5034 5445 2311
Size: 1904 398 274 147 411 2648
Fragments arranged by size:
2648 1904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 1186
Size: 1129 4653
BseYI C'CCAG_C
Cuts at: 4985 5481 4985
Size: 496 5286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2453 1163
Size: 573 417 300 4492
Fragments arranged by size:
4492 573 417 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2656 3843 3928 4999 2127
Size: 529 1187 85 1071 2910
Fragments arranged by size:
2910 1187 1071 529 85
BsiWI C'GTAC_G
Cuts at: 2540 2540
Size: 5782
BsmI GAATG_Cn'
Cuts at: 2430 3293 3386 2430
Size: 863 93 4826
Fragments arranged by size:
4826 863 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2514 2531 2656 2918 3067 3101
Size: 399 337 387 17 125 262 149 34
Cuts at: 3101 3843 3928 4999 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 337 262 149
125 85 34 17
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 3137 844
Size: 1331 962 3489
Fragments arranged by size:
3489 1331 962
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 2685 3669 1394
Size: 429 539 323 984 3507
Fragments arranged by size:
3507 984 539 429 323
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2448 4426 4613 1700
Size: 110 638 1978 187 2869
Fragments arranged by size:
2869 1978 638 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2372 794
Size: 1578 4204
BstBI TT'CG_AA
Cuts at: 2596 4586 2596
Size: 1990 3792
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777
Size: 1140 367 385 17 1452 86 12 346
Cuts at: 5777 1972
Size: 1977
Fragments arranged by size:
1977 1452 1140 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 513
Size: 552 939 606 3685
Fragments arranged by size:
3685 939 606 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 2918 3298 4092 4112 2382
Size: 536 380 794 20 4052
Fragments arranged by size:
4052 794 536 380 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3712 1353
Size: 719 1640 3423
Fragments arranged by size:
3423 1640 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2841 3845 4442 5229 5497 1239
Size: 393 1209 1004 597 787 268 1524
Fragments arranged by size:
1524 1209 1004 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 5782
EcoRI G'AATT_C
Cuts at: 3013 3091 3469 3013
Size: 78 378 5326
Fragments arranged by size:
5326 378 78
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2847 4929 1678
Size: 1169 2082 2531
Fragments arranged by size:
2531 2082 1169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2664 2696 4423 4455 4497
Size: 32 135 32 1092 32 1727 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1727 1092 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2180 2229 748
Size: 1432 49 4301
Fragments arranged by size:
4301 1432 49
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2932 4896 5080 1305
Size: 1627 1964 184 2007
Fragments arranged by size:
2007 1964 1627 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2777 3740 4099 4258 518
Size: 648 1035 576 963 359 159 2042
Fragments arranged by size:
2042 1035 963 648 576 359 159
MspA1I CmG'CkG
Cuts at: 1606 2147 2705 2714 2861 3198 3875 5023 5268
Size: 541 558 9 147 337 677 1148 245
Cuts at: 5268 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 558 541 337 245 147
9
NaeI GCC'GGC
Cuts at: 2450 2450
Size: 5782
NcoI C'CATG_G
Cuts at: 513 1065 2004 2610 513
Size: 552 939 606 3685
Fragments arranged by size:
3685 939 606 552
NdeI CA'TA_TG
Cuts at: 387 2647 387
Size: 2260 3522
NgoMIV G'CCGG_C
Cuts at: 2448 2448
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NsiI A_TGCA'T
Cuts at: 2430 2647 2430
Size: 217 5565
NspI r_CATG'y
Cuts at: 1132 1229 2432 3140 4685 1132
Size: 97 1203 708 1545 2229
Fragments arranged by size:
2229 1545 1203 708 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2840 1108
Size: 1732 4050
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2248 2248
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2881 2935 3134 830
Size: 2051 54 199 3478
Fragments arranged by size:
3478 2051 199 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2147 2861 2147
Size: 714 5068
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2043 2877 2931 3130 4265 4946 5137
Size: 219 998 834 54 199 1135 681 191
Cuts at: 5137 826
Size: 1471
Fragments arranged by size:
1471 1135 998 834 681 219 199 191
54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 2036 3109 1872
Size: 164 1073 4545
Fragments arranged by size:
4545 1073 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2760 3886 4787 5049 820
Size: 197 926 817 1126 901 262 1553
Fragments arranged by size:
1553 1126 926 901 817 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2610 3221 513
Size: 552 939 606 611 3074
Fragments arranged by size:
3074 939 611 606 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3075 3809 3826 3979 4164 4609 325
Size: 1255 1495 734 17 153 185 445 1498
Fragments arranged by size:
1498 1495 1255 734 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4028 5740 96
Size: 274 80 33 51 1443 2051 1712 138
Fragments arranged by size:
2051 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 2746 1316
Size: 1430 4352
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2245 2245
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 2034 3107 1870
Size: 164 1073 4545
Fragments arranged by size:
4545 1073 164
XmnI GAAnn'nnTTC
Cuts at: 2015 3911 2015
Size: 1896 3886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI