Restriction map for
pFN21AB8158
(Circular) (Six-base) MAPSORT of: pFN21AB8158.seq Check: 6444 from: 1 to: 5836
pFN21AB8158 5836 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2932 3646 278
Size: 53 83 186 936 1396 714 2468
Fragments arranged by size:
2468 1396 936 714 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 2220 2640 2808 3179 1937
Size: 271 12 420 168 371 4594
Fragments arranged by size:
4594 420 371 271 168 12
Acc65I G'GTAC_C
Cuts at: 2430 3157 2430
Size: 727 5109
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 3899 5283 5551 1632
Size: 929 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 929 268
AfeI AGC'GCT
Cuts at: 2117 2117
Size: 5836
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2523 3122 3579 5151 2523
Size: 599 457 1572 3208
Fragments arranged by size:
3208 1572 599 457
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5836
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 3036 3161 1870
Size: 73 1093 125 4545
Fragments arranged by size:
4545 1093 125 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 2430 2849 3157 4612
Size: 325 217 627 292 351 419 308 1455
Cuts at: 4612 618
Size: 1842
Fragments arranged by size:
1842 1455 627 419 351 325 308 292
217
BanII G_rGCy'C
Cuts at: 1391 2036 3093 3155 1391
Size: 645 1057 62 4072
Fragments arranged by size:
4072 1057 645 62
BbeI G_GCGC'C
Cuts at: 2083 2083
Size: 5836
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 928
Size: 971 487 4378
Fragments arranged by size:
4378 971 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4027 4061 1903
Size: 34 50 34 2006 34 3678
Fragments arranged by size:
3678 2006 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3007 3728 4938 3007
Size: 721 1210 3905
Fragments arranged by size:
3905 1210 721
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4447 136
Size: 107 122 71 631 605 2775 1525
Fragments arranged by size:
2775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2854 3897 5053 1790
Size: 246 818 1043 1156 2573
Fragments arranged by size:
2573 1156 1043 818 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4496 1239
Size: 3257 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 2929 3643 4025
Size: 53 83 186 936 547 849 714 382
Cuts at: 4025 4660 275
Size: 635 1451
Fragments arranged by size:
1451 936 849 714 635 547 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2082 2112 1178
Size: 30 165 30 679 30 4902
Fragments arranged by size:
4902 679 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5836
BseYI C'CCAG_C
Cuts at: 2029 2068 3084 5039 5535 2029
Size: 39 1016 1955 496 2330
Fragments arranged by size:
2330 1955 1016 496 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2845 1163
Size: 573 1109 4154
Fragments arranged by size:
4154 1109 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3045 3897 3982 5053 3045
Size: 852 85 1071 3828
Fragments arranged by size:
3828 1071 852 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
BsmBI CGTCTCn'nnnn_
Cuts at: 2470 2920 2470
Size: 450 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2854 3045 3093 3155 3897 3982
Size: 399 246 818 191 48 62 742 85
Cuts at: 3982 5053 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 818 742 399 246 191 85
62 48
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 2622 3725 5455 2622
Size: 1103 1730 3003
Fragments arranged by size:
3003 1730 1103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2136 2523 794
Size: 1342 387 4107
Fragments arranged by size:
4107 1342 387
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831
Size: 1194 367 385 17 1452 86 12 346
Cuts at: 5831 1972
Size: 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3067 513
Size: 552 2002 3282
Fragments arranged by size:
3282 2002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 2512 2554 3138 3352 4146 4166 2179
Size: 333 42 584 214 794 20 3849
Fragments arranged by size:
3849 794 584 333 214 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3283 4172 5801
Size: 54 1425 50 275 18 1453 889 1629
Cuts at: 5801 8
Size: 43
Fragments arranged by size:
1629 1453 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3766 1353
Size: 2413 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2412 4423 4797 4943 387
Size: 1064 138 453 370 2011 374 146 1280
Fragments arranged by size:
2011 1280 1064 453 374 370 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2561 3899 4496 5283 5551 1239
Size: 393 929 1338 597 787 268 1524
Fragments arranged by size:
1524 1338 929 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2662 2662
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
EcoRV GAT'ATC
Cuts at: 2455 2455
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2083 2119 2125 4983 1678
Size: 405 36 6 2858 2531
Fragments arranged by size:
2858 2531 405 36 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373
Size: 32 135 32 2905 32 42 32 2626
Fragments arranged by size:
2905 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KasI G'GCGC_C
Cuts at: 2079 2079
Size: 5836
KpnI G_GTAC'C
Cuts at: 2434 3161 2434
Size: 727 5109
MfeI C'AATT_G
Cuts at: 2572 3435 2572
Size: 863 4973
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4014
Fragments arranged by size:
4014 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3072 3794 4153 4312 518
Size: 648 1906 722 359 159 2042
Fragments arranged by size:
2042 1906 722 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2085 2183 2235 3252 3929 5077 5322
Size: 411 68 98 52 1017 677 1148 245
Cuts at: 5322 1606
Size: 2120
Fragments arranged by size:
2120 1148 1017 677 411 245 98 68
52
NarI GG'CG_CC
Cuts at: 2080 2080
Size: 5836
NcoI C'CATG_G
Cuts at: 513 1065 3067 513
Size: 552 2002 3282
Fragments arranged by size:
3282 2002 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 2870 3194 4739 1132
Size: 97 1641 324 1545 2229
Fragments arranged by size:
2229 1641 1545 324 97
PasI CC'CwG_GG
Cuts at: 1348 2440 2845 3099 1348
Size: 1092 405 254 4085
Fragments arranged by size:
4085 1092 405 254
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2670 2670
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2662 2662
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2519 2648 3188 830
Size: 1689 129 540 3478
Fragments arranged by size:
3478 1689 540 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2365 2515 2644 2723 2936 3184 4319
Size: 219 1320 150 129 79 213 248 1135
Cuts at: 4319 5000 5191 826
Size: 681 191 1471
Fragments arranged by size:
1471 1320 1135 681 248 219 213 191
150 129 79
SfoI GGC'GCC
Cuts at: 2081 2081
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3940 4841 5103 820
Size: 197 926 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 2870 3194 1132
Size: 1738 324 3774
Fragments arranged by size:
3774 1738 324
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2943 3067 3275 513
Size: 552 1878 124 208 3074
Fragments arranged by size:
3074 1878 552 208 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 2837 2996 3091 3863 3880 4033
Size: 1255 1199 58 159 95 772 17 153
Cuts at: 4033 4218 4663 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1199 772 445 185 159 153
95 58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2110 4082 5794
Size: 274 80 33 51 1443 133 1972 1712
Cuts at: 5794 96
Size: 138
Fragments arranged by size:
1972 1712 1443 274 138 133 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2082 2114 5796 5828 2082
Size: 32 3682 32 2090
Fragments arranged by size:
3682 2090 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2349 2349
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 2999 3965 2999
Size: 966 4870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2930 3644 276
Size: 53 83 186 936 1396 714 2468
Fragments arranged by size:
2468 1396 936 714 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI