Restriction map for
pFN21AB8155
(Circular) (Six-base) MAPSORT of: pFN21AB8155.seq Check: 9422 from: 1 to: 6085
pFN21AB8155 6085 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3028 3028
Size: 6085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3378 3895 278
Size: 53 83 186 936 1842 517 2468
Fragments arranged by size:
2468 1842 936 517 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 2933 4148 5532 5800 1632
Size: 876 425 1215 1384 268 1917
Fragments arranged by size:
1917 1384 1215 876 425 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 2776 4814 2776
Size: 2038 4047
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2820 1184
Size: 1636 4449
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 2667
Size: 32 6053
AlwNI CAG_nnn'CTG
Cuts at: 2150 2588 3828 5400 2150
Size: 438 1240 1572 2835
Fragments arranged by size:
2835 1572 1240 438
ApaI G_GGCC'C
Cuts at: 2234 2234
Size: 6085
ApaLI G'TGCA_C
Cuts at: 3302 4142 5298 3302
Size: 840 1156 4089
Fragments arranged by size:
4089 1156 840
ApoI r'AATT_y
Cuts at: 1493 3394 3620 3772 5881 1493
Size: 1901 226 152 2109 1697
Fragments arranged by size:
2109 1901 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2781 3410 1870
Size: 73 177 661 629 4545
Fragments arranged by size:
4545 661 629 177 73
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3406 4861 618
Size: 325 217 627 282 1337 1455 1842
Fragments arranged by size:
1842 1455 1337 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2234 2636 3299 3404 1391
Size: 843 402 663 105 4072
Fragments arranged by size:
4072 843 663 402 105
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 6085
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3310 928
Size: 971 1411 3703
Fragments arranged by size:
3703 1411 971
BbvCI CC'TCA_GC
Cuts at: 2061 2061
Size: 6085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2026 3977 5187 2026
Size: 1951 1210 2924
Fragments arranged by size:
2924 1951 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2385 4696 136
Size: 107 122 71 631 605 713 2311 1525
Fragments arranged by size:
2311 1525 713 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3366 6080 3366
Size: 2714 3371
BlpI GC'TnA_GC
Cuts at: 2164 2309 2581 2859 3293 3502 2164
Size: 145 272 278 434 209 4747
Fragments arranged by size:
4747 434 278 272 209 145
Bme1580I G_kGCm'C
Cuts at: 1790 2234 3306 4146 5302 1790
Size: 444 1072 840 1156 2573
Fragments arranged by size:
2573 1156 1072 840 444
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2770 3816
Size: 416 53 83 399 24 774 579 1046
Cuts at: 3816 4774 442
Size: 958 1753
Fragments arranged by size:
1753 1046 958 774 579 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3317 4745 1239
Size: 2078 1428 2579
Fragments arranged by size:
2579 2078 1428
Bpu10I CC'TnA_GC
Cuts at: 2061 2061
Size: 6085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2655 4210 5075 5373 2655
Size: 1555 865 298 3367
Fragments arranged by size:
3367 1555 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 882
Size: 617 3249 2219
Fragments arranged by size:
3249 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3199 3876 493
Size: 711 1995 677 2702
Fragments arranged by size:
2702 1995 711 677
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 3375 3892 4274
Size: 53 83 186 936 537 1305 517 382
Cuts at: 4274 4909 275
Size: 635 1451
Fragments arranged by size:
1451 1305 936 635 537 517 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2107 2128 2499 4518 4916 5190 5337 5748 2107
Size: 21 371 2019 398 274 147 411 2444
Fragments arranged by size:
2444 2019 411 398 371 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2522 2552 2708
Size: 30 165 30 867 30 222 30 156
Cuts at: 2708 2738 1178
Size: 30 4525
Fragments arranged by size:
4525 867 222 165 156 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 1186
Size: 928 5157
BseYI C'CCAG_C
Cuts at: 2233 2248 3088 5288 5784 2233
Size: 15 840 2200 496 2534
Fragments arranged by size:
2534 2200 840 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3048 3179 1163
Size: 573 1312 131 4069
Fragments arranged by size:
4069 1312 573 131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3144 3148 3535 4296 5324 1540
Size: 275 186 1143 4 387 761 1028 2301
Fragments arranged by size:
2301 1143 1028 761 387 275 186 4
BsiHKAI G_wGCw'C
Cuts at: 2310 3299 3306 4146 4231 5302 2310
Size: 989 7 840 85 1071 3093
Fragments arranged by size:
3093 1071 989 840 85 7
BsmI GAATG_Cn'
Cuts at: 3596 3689 3596
Size: 93 5992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2310 2636 3299 3306 3404 4146
Size: 399 444 76 326 663 7 98 742
Cuts at: 4146 4231 5302 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 444 399 326 98
85 76 7
BspEI T'CCGG_A
Cuts at: 2107 2128 2499 5748 2107
Size: 21 371 3249 2444
Fragments arranged by size:
3249 2444 371 21
BspHI T'CATG_A
Cuts at: 3267 3974 5704 3267
Size: 707 1730 3648
Fragments arranged by size:
3648 1730 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2402 3028 3304 3440 844
Size: 1558 626 276 136 3489
Fragments arranged by size:
3489 1558 626 276 136
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 1394
Size: 429 2149 3507
Fragments arranged by size:
3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 4754 65
Size: 1183 3332 174 1396
Fragments arranged by size:
3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2436 3147 4729 4916 1700
Size: 110 626 711 1582 187 2869
Fragments arranged by size:
2869 1582 711 626 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 2020 2255 2966 3380 4145 5157 2020
Size: 235 711 414 765 1012 2948
Fragments arranged by size:
2948 1012 765 711 414 235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2194 2496 2612 3366 3415 3782 4167 4184
Size: 222 302 116 754 49 367 385 17
Cuts at: 4184 5636 5722 5734 6080 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 754 385 367 346 302 222
116 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2114 2353 513
Size: 552 1049 239 4245
Fragments arranged by size:
4245 1049 552 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3106 487
Size: 683 56 1880 3466
Fragments arranged by size:
3466 1880 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 2438 2480 2717 3601 4395 4415 2350
Size: 88 42 237 884 794 20 4020
Fragments arranged by size:
4020 884 794 237 88 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2243 2278 529
Size: 35 1679 35 4336
Fragments arranged by size:
4336 1679 35 35
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2138 5092 809
Size: 507 822 2954 1802
Fragments arranged by size:
2954 1802 822 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2823 3141 3532
Size: 54 1425 50 275 18 993 318 391
Cuts at: 3532 4421 6050 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 993 889 391 318 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3141 3532 1537
Size: 275 1329 391 4090
Fragments arranged by size:
4090 1329 391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 4015 1353
Size: 843 1819 3423
Fragments arranged by size:
3423 1819 843
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2403 4672 5046 5192 387
Size: 1064 138 814 2269 374 146 1280
Fragments arranged by size:
2269 1280 1064 814 374 146 138
EcoICRI GAG'CTC
Cuts at: 3297 3297
Size: 6085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2933 3317 4148 4745 5532 5800
Size: 393 876 425 384 831 597 787 268
Cuts at: 5800 1239
Size: 1524
Fragments arranged by size:
1524 876 831 787 597 425 393 384
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 2912
Size: 6085
EcoO109I rG'GnC_Cy
Cuts at: 2230 2230
Size: 6085
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 3034 2350
Size: 684 5401
FspI TGC'GCA
Cuts at: 2444 3287 4591 2444
Size: 843 1304 3938
Fragments arranged by size:
3938 1304 843
HaeII r_GCGC'y
Cuts at: 1678 2073 2371 2959 3209 5232 1678
Size: 395 298 588 250 2023 2531
Fragments arranged by size:
2531 2023 588 395 298 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2522 2554 2762
Size: 32 135 32 698 32 220 32 208
Cuts at: 2762 2794 4726 4758 4800 4832 1373
Size: 32 1932 32 42 32 2626
Fragments arranged by size:
2626 1932 698 220 208 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3675 669
Size: 988 281 1491 246 3079
Fragments arranged by size:
3079 1491 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2757 3342 5199 5383 1305
Size: 1452 585 1857 184 2007
Fragments arranged by size:
2007 1857 1452 585 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2825 10
Size: 54 1425 343 993 3270
Fragments arranged by size:
3270 1425 993 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3266 4043 4402 4561 518
Size: 648 2100 777 359 159 2042
Fragments arranged by size:
2100 2042 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2313 2666 3247 3501 4178 5326 5571 1606
Size: 707 353 581 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 707 677 581 353 254 245
NaeI GCC'GGC
Cuts at: 2438 2438
Size: 6085
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 6085
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5533
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NgoMIV G'CCGG_C
Cuts at: 2436 2436
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 3265 3443 4988 1132
Size: 97 2036 178 1545 2229
Fragments arranged by size:
2229 2036 1545 178 97
PasI CC'CwG_GG
Cuts at: 1348 2761 1348
Size: 1413 4672
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2692 1108
Size: 1584 4501
PfoI T'CCnGG_A
Cuts at: 2838 3333 2838
Size: 495 5590
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 2230 2230
Size: 6085
PstI C_TGCA'G
Cuts at: 830 2174 2712 2883 3039 3437 830
Size: 1344 538 171 156 398 3478
Fragments arranged by size:
3478 1344 538 398 171 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2313 3247 2313
Size: 934 5151
SacI G_AGCT'C
Cuts at: 3299 3299
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SbfI CC_TGCA'GG
Cuts at: 3039 3437 3039
Size: 398 5687
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2170 2708 2879 3004 3035 3433 4568
Size: 219 1125 538 171 125 31 398 1135
Cuts at: 4568 5249 5440 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1125 681 538 398 219 191
171 125 31
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 6085
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 2122 3412 1872
Size: 250 1290 4545
Fragments arranged by size:
4545 1290 250
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2670 4189 5090 5352 820
Size: 197 926 727 1519 901 262 1553
Fragments arranged by size:
1553 1519 926 901 727 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3265 3443 1132
Size: 2133 178 3774
Fragments arranged by size:
3774 2133 178
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 3033 1517
Size: 768 748 4569
Fragments arranged by size:
4569 768 748
StyI C'CwwG_G
Cuts at: 513 1065 3524 513
Size: 552 2459 3074
Fragments arranged by size:
3074 2459 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 4112 4129 4282 4467 4912 325
Size: 1255 521 2011 17 153 185 445 1498
Fragments arranged by size:
2011 1498 1255 521 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4331 6043 96
Size: 274 80 33 51 1443 2354 1712 138
Fragments arranged by size:
2354 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6045 6077 6045
Size: 32 6053
Tth111I GACn'n_nGTC
Cuts at: 1316 2183 1316
Size: 867 5218
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 3085 2026
Size: 1059 5026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 2120 3410 1870
Size: 250 1290 4545
Fragments arranged by size:
4545 1290 250
XmnI GAAnn'nnTTC
Cuts at: 2996 4214 2996
Size: 1218 4867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3376 3893 276
Size: 53 83 186 936 1842 517 2468
Fragments arranged by size:
2468 1842 936 517 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI