Restriction map for
pFN21AB8154
(Circular) (Six-base) MAPSORT of: pFN21AB8154.seq Check: 111 from: 1 to: 6412
pFN21AB8154 6412 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4222 278
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3755 1937
Size: 1818 4594
Acc65I G'GTAC_C
Cuts at: 3733 3733
Size: 6412
AclI AA'CG_TT
Cuts at: 1563 2747 4539 4912 1563
Size: 1184 1792 373 3063
Fragments arranged by size:
3063 1792 1184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2818 2902 3154 3238 4475 5859 6127 1632
Size: 1186 84 252 84 1237 1384 268 1917
Fragments arranged by size:
1917 1384 1237 1186 268 252 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 5311 6288 1203
Size: 4108 977 1327
Fragments arranged by size:
4108 1327 977
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6412
AlwNI CAG_nnn'CTG
Cuts at: 4155 5727 4155
Size: 1572 4840
ApaLI G'TGCA_C
Cuts at: 3600 4469 5625 3600
Size: 869 1156 4387
Fragments arranged by size:
4387 1156 869
ApoI r'AATT_y
Cuts at: 1493 2044 2089 2264 2700 3036 3120 3456 3540
Size: 551 45 175 436 336 84 336 84
Cuts at: 3540 3624 3721 3947 4099 6208 1493
Size: 84 97 226 152 2109 1697
Fragments arranged by size:
2109 1697 551 436 336 336 226 175
152 97 84 84 84 45
AseI AT'TA_AT
Cuts at: 160 1032 2429 4966 160
Size: 872 1397 2537 1606
Fragments arranged by size:
2537 1606 1397 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 3737 1870
Size: 73 1794 4545
Fragments arranged by size:
4545 1794 73
BamHI G'GATC_C
Cuts at: 3742 4109 3742
Size: 367 6045
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3733 5188 618
Size: 325 217 627 1946 1455 1842
Fragments arranged by size:
1946 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2196 2678 3731 1391
Size: 805 482 1053 4072
Fragments arranged by size:
4072 1053 805 482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 2656 928
Size: 971 471 286 4684
Fragments arranged by size:
4684 971 471 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4603 4637 1903
Size: 34 2666 34 3678
Fragments arranged by size:
3678 2666 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2500 3092 4304 5514 2500
Size: 592 1212 1210 3398
Fragments arranged by size:
3398 1212 1210 592
BclI T'GATC_A
Cuts at: 1548 3274 1548
Size: 1726 4686
BfrBI ATG'CAT
Cuts at: 2726 2726
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5023 136
Size: 107 122 71 631 605 3351 1525
Fragments arranged by size:
3351 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 3024 3829 3024
Size: 805 5607
Bme1580I G_kGCm'C
Cuts at: 1790 3604 4473 5629 1790
Size: 1814 869 1156 2573
Fragments arranged by size:
2573 1814 1156 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2346 4143 5101
Size: 416 53 83 399 24 929 1797 958
Cuts at: 5101 442
Size: 1753
Fragments arranged by size:
1797 1753 958 929 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2730 2814 2898 2982 3150 3234 3318 3402
Size: 1491 84 84 84 168 84 84 84
Cuts at: 3402 3486 3570 5072 1239
Size: 84 84 1502 2579
Fragments arranged by size:
2579 1502 1491 168 84 84 84 84
84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2037 4537 5402 5700 2037
Size: 2500 865 298 2749
Fragments arranged by size:
2749 2500 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2962 3130 5075 882
Size: 617 1463 168 1945 2219
Fragments arranged by size:
2219 1945 1463 617 168
BsaAI yAC'GTr
Cuts at: 493 1204 4203 493
Size: 711 2999 2702
Fragments arranged by size:
2999 2702 711
BsaBI GATnn'nnATC
Cuts at: 3279 6406 3279
Size: 3127 3285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4219 4601 5236 275
Size: 53 83 186 936 2686 382 635 1451
Fragments arranged by size:
2686 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4845 5243 5517 5664 6075 4845
Size: 398 274 147 411 5182
Fragments arranged by size:
5182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2133 2163 1178
Size: 30 165 30 730 30 5427
Fragments arranged by size:
5427 730 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6412
BseYI C'CCAG_C
Cuts at: 2398 5615 6111 2398
Size: 3217 496 2699
Fragments arranged by size:
3217 2699 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5839
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3862 4623 5651 1540
Size: 275 186 1861 761 1028 2301
Fragments arranged by size:
2301 1861 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2678 3604 4473 4558 5629 2678
Size: 926 869 85 1071 3461
Fragments arranged by size:
3461 1071 926 869 85
BsmI GAATG_Cn'
Cuts at: 2848 3436 3923 4016 2848
Size: 588 487 93 5244
Fragments arranged by size:
5244 588 487 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2678 3604 3731 4473 4558 5629
Size: 399 406 482 926 127 742 85 1071
Cuts at: 5629 1391
Size: 2174
Fragments arranged by size:
2174 1071 926 742 482 406 399 127
85
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 3616 4301 6031 3616
Size: 685 1730 3997
Fragments arranged by size:
3997 1730 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3767 844
Size: 2923 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4299 1394
Size: 429 2476 3507
Fragments arranged by size:
3507 2476 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4907 5081 65
Size: 1183 3659 174 1396
Fragments arranged by size:
3659 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5056 5243 1700
Size: 110 3246 187 2869
Fragments arranged by size:
3246 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2094 3109 96
Size: 1998 1015 3399
Fragments arranged by size:
3399 1998 1015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 4472 5484 4472
Size: 1012 5400
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3188 3440 3608 794
Size: 2394 252 168 3598
Fragments arranged by size:
3598 2394 252 168
BstBI TT'CG_AA
Cuts at: 2976 5216 2976
Size: 2240 4172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6412
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 6412
BstYI r'GATC_y
Cuts at: 1972 3518 3742 4109 4494 4511 5963 6049 6061
Size: 1546 224 367 385 17 1452 86 12
Cuts at: 6061 6407 1972
Size: 346 1977
Fragments arranged by size:
1977 1546 1452 385 367 346 224 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5673
Fragments arranged by size:
5673 683 56
BtsI GCAGTG_nn'
Cuts at: 3928 4722 4742 3928
Size: 794 20 5598
Fragments arranged by size:
5598 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6377
DraI TTT'AAA
Cuts at: 2254 3716 2254
Size: 1462 4950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3859 4748 6377
Size: 54 1425 50 275 18 2029 889 1629
Cuts at: 6377 8
Size: 43
Fragments arranged by size:
2029 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3859 1537
Size: 275 2047 4090
Fragments arranged by size:
4090 2047 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2694 4342 1353
Size: 1341 1648 3423
Fragments arranged by size:
3423 1648 1341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4999 5373 5519 387
Size: 1064 138 3410 374 146 1280
Fragments arranged by size:
3410 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2676 2676
Size: 6412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2730 2814 2818 2898 2902 2982 3150
Size: 393 1098 84 4 80 4 80 168
Cuts at: 3150 3154 3234 3238 3318 3402 3486 3570 4475
Size: 4 80 4 80 84 84 84 905
Cuts at: 4475 5072 5859 6127 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1098 905 787 597 393 268 168
84 84 84 84 80 80 80 80
4 4 4 4
EcoRI G'AATT_C
Cuts at: 2700 3036 3120 3456 3540 3624 3721 4099 2700
Size: 336 84 336 84 84 97 378 5013
Fragments arranged by size:
5013 378 336 336 97 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2468 2468
Size: 6412
FspI TGC'GCA
Cuts at: 4918 4918
Size: 6412
HaeII r_GCGC'y
Cuts at: 1678 5559 1678
Size: 3881 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2929 2961 5053
Size: 32 135 32 581 32 744 32 2092
Cuts at: 5053 5085 5127 5159 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2092 744 581 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4002 669
Size: 988 281 1818 246 3079
Fragments arranged by size:
3079 1818 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KpnI G_GTAC'C
Cuts at: 3737 3737
Size: 6412
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5526 5710 1305
Size: 786 3435 184 2007
Fragments arranged by size:
3435 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4590
Fragments arranged by size:
4590 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 4370 4729 4888 518
Size: 648 1393 1811 359 159 2042
Fragments arranged by size:
2042 1811 1393 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2398 3828 4505 5653 5898 1606
Size: 792 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 792 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5860
NdeI CA'TA_TG
Cuts at: 387 2287 387
Size: 1900 4512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NsiI A_TGCA'T
Cuts at: 2728 2728
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 2730 3770 5315 1132
Size: 97 1501 1040 1545 2229
Fragments arranged by size:
2229 1545 1501 1040 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6412
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 3982 3982
Size: 6412
PstI C_TGCA'G
Cuts at: 830 3764 830
Size: 2934 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
PvuII CAG'CTG
Cuts at: 2398 2398
Size: 6412
SacI G_AGCT'C
Cuts at: 2678 2678
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 4660 6372 4660
Size: 1712 4700
SfcI C'TryA_G
Cuts at: 826 1045 2119 3511 3696 3760 4895 5576 5767
Size: 219 1074 1392 185 64 1135 681 191
Cuts at: 5767 826
Size: 1471
Fragments arranged by size:
1471 1392 1135 1074 681 219 191 185
64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6412
SmaI CCC'GGG
Cuts at: 1872 3739 1872
Size: 1867 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2052 4516 5417 5679 820
Size: 197 926 109 2464 901 262 1553
Fragments arranged by size:
2464 1553 926 901 262 197 109
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 3770 1132
Size: 2638 3774
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 2999 3335 3438 1517
Size: 1482 336 103 4491
Fragments arranged by size:
4491 1482 336 103
StyI C'CwwG_G
Cuts at: 513 1065 2010 3851 513
Size: 552 945 1841 3074
Fragments arranged by size:
3074 1841 945 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4439 4456 4609 4794 5239 325
Size: 1255 2859 17 153 185 445 1498
Fragments arranged by size:
2859 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3109 4658
Size: 274 80 33 51 1443 117 1015 1549
Cuts at: 4658 6370 96
Size: 1712 138
Fragments arranged by size:
1712 1549 1443 1015 274 138 117 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6372 6404 6372
Size: 32 6380
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 3748 3748
Size: 6412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2235 2235
Size: 6412
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6412
XmaI C'CCGG_G
Cuts at: 1870 3737 1870
Size: 1867 4545
XmnI GAAnn'nnTTC
Cuts at: 2451 2663 2747 2831 3167 3251 3587 4541 2451
Size: 212 84 84 336 84 336 954 4322
Fragments arranged by size:
4322 954 336 336 212 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4220 276
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI