Restriction map for
pFN21AB8153
(Circular) (Six-base) MAPSORT of: pFN21AB8153.seq Check: 5244 from: 1 to: 5944
pFN21AB8153 5944 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3287 1937
Size: 1350 4594
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2712 4007 5391 5659 1632
Size: 1080 1295 1384 268 1917
Fragments arranged by size:
1917 1384 1295 1080 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 2107 4673 2107
Size: 2566 3378
AleI CACnn'nnGTG
Cuts at: 1166 2786 1166
Size: 1620 4324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3216 1184
Size: 2032 3912
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 2700
Size: 32 5912
AlwNI CAG_nnn'CTG
Cuts at: 2098 2161 3687 5259 2098
Size: 63 1526 1572 2783
Fragments arranged by size:
2783 1572 1526 63
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 2648 3253 3479 3631 5740 1493
Size: 1155 605 226 152 2109 1697
Fragments arranged by size:
2109 1697 1155 605 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3081 4498 160
Size: 872 2049 1417 1606
Fragments arranged by size:
2049 1606 1417 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 2239 2330 2566 3129 3269 1870
Size: 73 296 91 236 563 140 4545
Fragments arranged by size:
4545 563 296 236 140 91 73
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2148 2302 2347 2804
Size: 325 217 627 255 106 154 45 457
Cuts at: 2804 3125 3265 4720 618
Size: 321 140 1455 1842
Fragments arranged by size:
1842 1455 627 457 325 321 255 217
154 140 106 45
BanII G_rGCy'C
Cuts at: 1391 2228 3263 1391
Size: 837 1035 4072
Fragments arranged by size:
4072 1035 837
BbeI G_GCGC'C
Cuts at: 2046 2152 2306 2351 2046
Size: 106 154 45 5639
Fragments arranged by size:
5639 154 106 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 928
Size: 971 801 4172
Fragments arranged by size:
4172 971 801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2334 4555 136
Size: 107 122 71 631 605 662 2221 1525
Fragments arranged by size:
2221 1525 662 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 2313 2433 3361 2313
Size: 120 928 4896
Fragments arranged by size:
4896 928 120
Bme1580I G_kGCm'C
Cuts at: 1790 2807 4005 5161 1790
Size: 1017 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 1017
BmgBI CAC'GTC
Cuts at: 1318 2558 1318
Size: 1240 4704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3675 4633 442
Size: 416 53 83 399 24 2258 958 1753
Fragments arranged by size:
2258 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2808 3095 4604 1239
Size: 1569 287 1509 2579
Fragments arranged by size:
2579 1569 1509 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4069 4934 5232 4069
Size: 865 298 4781
Fragments arranged by size:
4781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4607 882
Size: 617 3108 2219
Fragments arranged by size:
3108 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3735 493
Size: 711 2531 2702
Fragments arranged by size:
2702 2531 711
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2149 2303 2348
Size: 53 83 186 936 510 106 154 45
Cuts at: 2348 3751 4133 4768 275
Size: 1403 382 635 1451
Fragments arranged by size:
1451 1403 936 635 510 382 186 154
106 83 53 45
BsaWI w'CCGG_w
Cuts at: 2937 3042 4377 4775 5049 5196 5607 2937
Size: 105 1335 398 274 147 411 3274
Fragments arranged by size:
3274 1335 411 398 274 147 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5719
Fragments arranged by size:
5719 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2807 2831 3135 1186
Size: 1621 24 304 3995
Fragments arranged by size:
3995 1621 304 24
BseYI C'CCAG_C
Cuts at: 2227 3069 5147 5643 2227
Size: 842 2078 496 2528
Fragments arranged by size:
2528 2078 842 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2748 1163
Size: 573 1012 4359
Fragments arranged by size:
4359 1012 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 2266 3394 4155 5183 1540
Size: 275 186 198 67 1128 761 1028 2301
Fragments arranged by size:
2301 1128 1028 761 275 198 186 67
BsiHKAI G_wGCw'C
Cuts at: 4005 4090 5161 4005
Size: 85 1071 4788
Fragments arranged by size:
4788 1071 85
BsmI GAATG_Cn'
Cuts at: 3455 3548 3455
Size: 93 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2807 3263 4005 4090 5161 1391
Size: 399 438 579 456 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 456 438 399 85
BspEI T'CCGG_A
Cuts at: 2937 3042 5607 2937
Size: 105 2565 3274
Fragments arranged by size:
3274 2565 105
BspHI T'CATG_A
Cuts at: 3833 5563 3833
Size: 1730 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3299 844
Size: 2455 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 3831 1394
Size: 429 614 1394 3507
Fragments arranged by size:
3507 1394 614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4439 4613 65
Size: 1183 3191 174 1396
Fragments arranged by size:
3191 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 2204 2252 4588 4775 1700
Size: 110 335 59 48 2336 187 2869
Fragments arranged by size:
2869 2336 335 187 110 59 48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 4004 5016 4004
Size: 1012 4932
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 794
Size: 1367 4577
BstBI TT'CG_AA
Cuts at: 2719 4748 2719
Size: 2029 3915
BstEII G'GTnAC_C
Cuts at: 1184 3089 1184
Size: 1905 4039
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 2788 2971 2752
Size: 36 183 5725
Fragments arranged by size:
5725 183 36
BstYI r'GATC_y
Cuts at: 1972 2441 3274 3641 4026 4043 5495 5581 5593
Size: 469 833 367 385 17 1452 86 12
Cuts at: 5593 5939 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 833 469 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2212 2404 2970 513
Size: 552 963 184 192 566 3487
Fragments arranged by size:
3487 963 566 552 192 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2063 487
Size: 683 56 837 4368
Fragments arranged by size:
4368 837 683 56
BtsI GCAGTG_nn'
Cuts at: 3460 4254 4274 3460
Size: 794 20 5130
Fragments arranged by size:
5130 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2686 2721 529
Size: 35 2122 35 3752
Fragments arranged by size:
3752 2122 35 35
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4951 809
Size: 507 3635 1802
Fragments arranged by size:
3635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2196 2263
Size: 54 1425 50 275 18 246 120 67
Cuts at: 2263 3391 4280 5909 8
Size: 1128 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 275 246 120 67
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2196 2263 3391 1537
Size: 275 384 67 1128 4090
Fragments arranged by size:
4090 1128 384 275 67
EarI CTCTTCn'nnn_
Cuts at: 1353 2368 2808 3874 1353
Size: 1015 440 1066 3423
Fragments arranged by size:
3423 1066 1015 440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4531 4905 5051 387
Size: 1064 138 2942 374 146 1280
Fragments arranged by size:
2942 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2712 2808 3095 4007 4604 5391 5659
Size: 393 1080 96 287 912 597 787 268
Cuts at: 5659 1239
Size: 1524
Fragments arranged by size:
1524 1080 912 787 597 393 287 268
96
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2373 2132
Size: 241 5703
EcoO109I rG'GnC_Cy
Cuts at: 2235 2235
Size: 5944
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 2016 2046 2146 2152 2306 2351 5091 1678
Size: 338 30 100 6 154 45 2740 2531
Fragments arranged by size:
2740 2531 338 154 100 45 30 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2426 2458 3050
Size: 32 135 32 521 32 301 32 592
Cuts at: 3050 3082 4585 4617 4659 4691 1373
Size: 32 1503 32 42 32 2626
Fragments arranged by size:
2626 1503 592 521 301 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3288 3534 669
Size: 988 281 1350 246 3079
Fragments arranged by size:
3079 1350 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5944
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KasI G'GCGC_C
Cuts at: 2042 2148 2302 2347 2042
Size: 106 154 45 5639
Fragments arranged by size:
5639 154 106 45
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2823 5058 5242 1305
Size: 786 732 2235 184 2007
Fragments arranged by size:
2235 2007 786 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 3876
Fragments arranged by size:
3876 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2786 3902 4261 4420 518
Size: 648 1620 1116 359 159 2042
Fragments arranged by size:
2042 1620 1116 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2095 2161 2214 2317 2495 3073 3360
Size: 433 56 66 53 103 178 578 287
Cuts at: 3360 4037 5185 5430 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 578 433 287 245 178
103 66 56 53
NaeI GCC'GGC
Cuts at: 2147 2254 2147
Size: 107 5837
NarI GG'CG_CC
Cuts at: 2043 2149 2303 2348 2043
Size: 106 154 45 5639
Fragments arranged by size:
5639 154 106 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5392
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NgoMIV G'CCGG_C
Cuts at: 2145 2252 2145
Size: 107 5837
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 3302 4847 1132
Size: 97 2073 1545 2229
Fragments arranged by size:
2229 2073 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2603 2800 1348
Size: 1255 197 4492
Fragments arranged by size:
4492 1255 197
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5944
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PstI C_TGCA'G
Cuts at: 830 2166 2346 3296 830
Size: 1336 180 950 3478
Fragments arranged by size:
3478 1336 950 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
PvuII CAG'CTG
Cuts at: 2095 2161 2495 3073 2095
Size: 66 334 578 4966
Fragments arranged by size:
4966 578 334 66
SacII CC_GC'GG
Cuts at: 2215 2215
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 2346 3296 2346
Size: 950 4994
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SexAI A'CCwGG_T
Cuts at: 2518 2518
Size: 5944
SfcI C'TryA_G
Cuts at: 826 1045 2162 2342 2446 3292 4427 5108 5299
Size: 219 1117 180 104 846 1135 681 191
Cuts at: 5299 826
Size: 1471
Fragments arranged by size:
1471 1135 1117 846 681 219 191 180
104
SfoI GGC'GCC
Cuts at: 2044 2150 2304 2349 2044
Size: 106 154 45 5639
Fragments arranged by size:
5639 154 106 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 2145 1700
Size: 445 5499
SmaI CCC'GGG
Cuts at: 1872 2332 3131 3271 1872
Size: 460 799 140 4545
Fragments arranged by size:
4545 799 460 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4048 4949 5211 820
Size: 197 926 2105 901 262 1553
Fragments arranged by size:
2105 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5944
StyI C'CwwG_G
Cuts at: 513 1065 3383 513
Size: 552 2318 3074
Fragments arranged by size:
3074 2318 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2693 3971 3988 4141 4326 4771 325
Size: 1255 1113 1278 17 153 185 445 1498
Fragments arranged by size:
1498 1278 1255 1113 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4190 5902 96
Size: 274 80 33 51 1443 2213 1712 138
Fragments arranged by size:
2213 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3181 3181
Size: 5944
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 2330 3129 3269 1870
Size: 460 799 140 4545
Fragments arranged by size:
4545 799 460 140
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SfiI SrfI SwaI