Restriction map for
pFN21AB8146
(Circular) (Six-base) MAPSORT of: pFN21AB8146.seq Check: 3954 from: 1 to: 5824
pFN21AB8146 5824 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2462 2708 3887 5271 5539 1632
Size: 758 72 246 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1179 758 268 246 72
AfeI AGC'GCT
Cuts at: 2485 2485
Size: 5824
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 3064 4553 3064
Size: 1489 4335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 2075 2674 3032 3567 5139 2075
Size: 599 358 535 1572 2760
Fragments arranged by size:
2760 1572 599 535 358
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 3149 1870
Size: 73 184 1022 4545
Fragments arranged by size:
4545 1022 184 73
BamHI G'GATC_C
Cuts at: 2161 3154 3521 2161
Size: 993 367 4464
Fragments arranged by size:
4464 993 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 3145 4600 618
Size: 325 217 627 364 994 1455 1842
Fragments arranged by size:
1842 1455 994 627 364 325 217
BanII G_rGCy'C
Cuts at: 1391 3143 1391
Size: 1752 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2312 2346 4015 4049 1903
Size: 34 375 34 1669 34 3678
Fragments arranged by size:
3678 1669 375 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BfrBI ATG'CAT
Cuts at: 2576 2576
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 2703 3241 2703
Size: 538 5286
Bme1580I G_kGCm'C
Cuts at: 1790 2154 3885 5041 1790
Size: 364 1731 1156 2573
Fragments arranged by size:
2573 1731 1156 364
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 2938 3555
Size: 416 53 83 399 24 975 546 617
Cuts at: 3555 4513 442
Size: 958 1753
Fragments arranged by size:
1753 975 958 617 546 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2201 1056
Size: 1145 4679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3038
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 4484 1239
Size: 974 2271 2579
Fragments arranged by size:
2579 2271 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3949 4814 5112 2168
Size: 1781 865 298 2880
Fragments arranged by size:
2880 1781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2370 2400 1178
Size: 30 165 30 967 30 4602
Fragments arranged by size:
4602 967 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 2738 1186
Size: 903 649 4272
Fragments arranged by size:
4272 903 649
BseYI C'CCAG_C
Cuts at: 2068 2769 5027 5523 2068
Size: 701 2258 496 2369
Fragments arranged by size:
2369 2258 701 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2494 1163
Size: 573 758 4493
Fragments arranged by size:
4493 758 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3885 3970 5041 3885
Size: 85 1071 4668
Fragments arranged by size:
4668 1071 85
BsmI GAATG_Cn'
Cuts at: 2578 3335 3428 2578
Size: 757 93 4974
Fragments arranged by size:
4974 757 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 3143 3885 3970 5041 1391
Size: 399 364 989 742 85 1071 2174
Fragments arranged by size:
2174 1071 989 742 399 364 85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2098 3179 844
Size: 1254 1081 3489
Fragments arranged by size:
3489 1254 1081
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2362 4319 4493 65
Size: 1183 1114 1957 174 1396
Fragments arranged by size:
1957 1396 1183 1114 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 2851 1184
Size: 1667 4157
BstXI CCAn_nnnn'nTGG
Cuts at: 2382 2644 2382
Size: 262 5562
BstYI r'GATC_y
Cuts at: 1972 2161 2914 3154 3521 3906 3923 5375 5461
Size: 189 753 240 367 385 17 1452 86
Cuts at: 5461 5473 5819 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 753 385 367 346 240 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2155 2774 2847 3120 513
Size: 552 1090 619 73 273 3217
Fragments arranged by size:
3217 1090 619 552 273 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2057 3340 4134 4154 2057
Size: 1283 794 20 3727
Fragments arranged by size:
3727 1283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2997 2997
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2691 4831 809
Size: 507 1375 2140 1802
Fragments arranged by size:
2140 1802 1375 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2868 3271 4160
Size: 54 1425 50 275 18 1038 403 889
Cuts at: 4160 5789 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1038 889 403 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2481 2684 2715 3754 1353
Size: 1128 203 31 1039 3423
Fragments arranged by size:
3423 1128 1039 203 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2104 2434 2999 4411 4785 4931
Size: 1064 138 515 330 565 1412 374 146
Cuts at: 4931 387
Size: 1280
Fragments arranged by size:
1412 1280 1064 565 515 374 330 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2390 2462 2708 3887 4484 5271
Size: 393 581 177 72 246 1179 597 787
Cuts at: 5271 5539 1239
Size: 268 1524
Fragments arranged by size:
1524 1179 787 597 581 393 268 246
177 72
EcoO109I rG'GnC_Cy
Cuts at: 3046 3046
Size: 5824
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
EcoRV GAT'ATC
Cuts at: 2665 2665
Size: 5824
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2141 2487 4971 1678
Size: 463 346 2484 2531
Fragments arranged by size:
2531 2484 463 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2205 2237 2649 2681 3050
Size: 32 135 32 633 32 412 32 369
Cuts at: 3050 3082 4465 4497 4539 4571 1373
Size: 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 633 412 369 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2293 2604 4938 5122 1305
Size: 988 311 2334 184 2007
Fragments arranged by size:
2334 2007 988 311 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2870 10
Size: 54 1425 343 1038 2964
Fragments arranged by size:
2964 1425 1038 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2642 3782 4141 4300 518
Size: 648 1101 375 1140 359 159 2042
Fragments arranged by size:
2042 1140 1101 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2586 3052 3240 3917 5065 5310 1606
Size: 980 466 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 980 677 466 245 188
NcoI C'CATG_G
Cuts at: 513 1065 2847 3120 513
Size: 552 1782 273 3217
Fragments arranged by size:
3217 1782 552 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 2197 1052
Size: 1145 4679
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NsiI A_TGCA'T
Cuts at: 2578 2578
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2711 2767 3182 4727 1132
Size: 97 1482 56 415 1545 2229
Fragments arranged by size:
2229 1545 1482 415 97 56
PasI CC'CwG_GG
Cuts at: 1348 2494 2623 1348
Size: 1146 129 4549
Fragments arranged by size:
4549 1146 129
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2075 1108
Size: 967 4857
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2417 2449 3893 3925 5406 5438 2417
Size: 32 1444 32 1481 32 2803
Fragments arranged by size:
2803 1481 1444 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3046 3046
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2018 2550 3057 3176 830
Size: 1188 532 507 119 3478
Fragments arranged by size:
3478 1188 532 507 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2014 2518 2546 3053 3172 4307 4988
Size: 219 969 504 28 507 119 1135 681
Cuts at: 4988 5179 826
Size: 191 1471
Fragments arranged by size:
1471 1135 969 681 507 504 219 191
119 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2129 3151 1872
Size: 257 1022 4545
Fragments arranged by size:
4545 1022 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 3928 4829 5091 820
Size: 197 926 240 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 240 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2169 2275 2847 3072 3120 3263 513
Size: 552 1104 106 572 225 48 143 3074
Fragments arranged by size:
3074 1104 572 552 225 143 106 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 2438 2486 3851 3868 4021 4206
Size: 1255 794 64 48 1365 17 153 185
Cuts at: 4206 4651 325
Size: 445 1498
Fragments arranged by size:
1498 1365 1255 794 445 185 153 64
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2311 2343 2370 2402 5784 5816 2311
Size: 32 27 32 3382 32 2319
Fragments arranged by size:
3382 2319 32 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2587 2587
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2127 3149 1870
Size: 257 1022 4545
Fragments arranged by size:
4545 1022 257
XmnI GAAnn'nnTTC
Cuts at: 2750 3092 3953 2750
Size: 342 861 4621
Fragments arranged by size:
4621 861 342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI